EMAGE mouse gene expression spatial database

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EMAGE:18255

BC049730 cDNA sequence BC049730 ( MGI:2681843)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012041. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012041_01	euxassay_012041_02	euxassay_012041_03	euxassay_012041_04

euxassay_012041_05	euxassay_012041_06	euxassay_012041_07	euxassay_012041_08	euxassay_012041_09

euxassay_012041_10	euxassay_012041_11	euxassay_012041_12	euxassay_012041_13	euxassay_012041_14

euxassay_012041_15	euxassay_012041_16	euxassay_012041_17	euxassay_012041_18	euxassay_012041_19

euxassay_012041_20	euxassay_012041_21	euxassay_012041_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18255_wholemount_strong.wlz

18255_wholemount_moderate.wlz

18255_wholemount_weak.wlz

18255_wholemount_possible.wlz

18255_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18255_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

moderate

regional

moderate expression: see section 01 weak expression: see section 02 19 20 21 22

hip

weak

regional

weak expression: see section 09 15 16

tibia

weak

regional

weak expression: see section 01 02 03

femur

moderate

regional

moderate expression: see section 19 20 22 weak expression: see section 02 03 04 06 07 21

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 07 08 09 16 17 18 19 20 weak expression: see section 05 06 14 21

exoccipital bone

weak

regional

weak expression: see section 03 04 16 17 18 19 20

temporal bone

moderate

regional

moderate expression: see section 21 22 weak expression: see section 01 02 03 19 20

vault of skull

weak

regional

weak expression: see section 01 02

orbito-sphenoid

moderate

regional

moderate expression: see section 22 weak expression: see section 01 02 04 07 17 18 19

viscerocranium

weak

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 01 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9791
Entity Detected:	BC049730, cDNA sequence BC049730 ( MGI:2681843)
Sequence:	sense strand is shown >T9791 AGAGCACAACTGTAAGCATCTTCAAAGGCTGAGATGCTGAGGGTTTCTGGGGCTATTGTCCTGAATCTCG ACCCTGAAGGAACACAGTCGCTGGGATCTTGGTCTTGGGGAAGCCATAGCTGAGACTGAATGTTCGGACA GAGAACCGGAGGCAGATTCCCAGGTCCTGATCAGCTCTTCCCAGCCTCAGAGAAGCCATGGGAGCCTGCC GCATCCAGTATGTCCTGCTCCTCTCTCTGCTGGGCTTCTGCCCCTGCTCAGATACTCTGAACTGTCAGGA AGGATCCGTGATGAAGTTTGGCCAAAACTTCAGTAGGACATCAATTGAATGGATCGTAAATCAGACAGTT GGGTCAGAGAAGAATAAAGGAGAGATGTGTCAGGAGATATTTCTGCTCATAGATGTAGGTGAAAAATCTC TCATATTGGGGACCAAAGGCATCACCACTGTTCCTGGTAAGAGAAAAAAAACAGATGTGTTTTCCCCTGG ACCTGGAATAGTGAGTGCATCTTATGTCCACTTCTGTGACACTGATCTGTGCAACAATGCTAGCAGCACC AAGGTCCTACTTGACCTCTTGAAGCTTTCAGAGTACAATGAGCCAGGAACCACCAAGTGTCCTGTCTGCT TGGATTTTAAGGGTTCTTGCAATACAGAGTCCAACCATACCTTGTGCCCTAAGGACACGAAATGCTATGC AGCTTCCTTGGGAGTGCATGGAGGTGACCTCAGTGCCATTTTTAGCATTTCTGGATGCCTGAACTCATCT CATACATACTTATTGAACAACCAAAATACAATTGGAATCATCAGTATAAAAGAAACCCTTGAGTCAAATG TCACTTTATCCTCACAGGGACTACTTGTCCCGAGCATCTTACTGACCTGGATGTTTGGGCTCTTAGCCCT TTGTTTACAGAGATCTGTCCTCTGCTGAACCCATTTCCTGGCCATTCTTAATCCCTCTTGGCCACCCAGA CCCAGCCCTCCTCTGACTTCAAAGACCCGAGGCTGGGCGCTGTTGGCCTGCGCTGTCGGGGCCCTCATCC CTCACCCACACACCTCTGCCCCTCATGCTTAGACTTGGGAGCCACTGAACCCAGCCGGCTCCAGAGCAGA CAAGCACACGTTGACCACGTGTGGAAAAATAAAGTTGCTGTTGTTTGTCCAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6770790 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6770790 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18256 same embryo
	EMAGE:18257 same embryo
	EMAGE:18252 same embryo
	EMAGE:18253 same embryo
	EMAGE:18251 same embryo
	EMAGE:18254 same embryo
	EurExpress:euxassay_012041 same experiment
	MGI:4823428 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source