EMAGE mouse gene expression spatial database

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EMAGE:18262

Efcab6 EF-hand calcium binding domain 6 ( MGI:1924877)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012039. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012039_01	euxassay_012039_02	euxassay_012039_03	euxassay_012039_04

euxassay_012039_05	euxassay_012039_06	euxassay_012039_07	euxassay_012039_08	euxassay_012039_09

euxassay_012039_10	euxassay_012039_11	euxassay_012039_12	euxassay_012039_13	euxassay_012039_14

euxassay_012039_15	euxassay_012039_16	euxassay_012039_17	euxassay_012039_18	euxassay_012039_19

euxassay_012039_20	euxassay_012039_21	euxassay_012039_22	euxassay_012039_23	euxassay_012039_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18262_wholemount_strong.wlz

18262_wholemount_moderate.wlz

18262_wholemount_weak.wlz

18262_wholemount_possible.wlz

18262_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18262_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9502
Entity Detected:	Efcab6, EF-hand calcium binding domain 6 ( MGI:1924877)
Sequence:	sense strand is shown >T9502 GAAGACTTGGCTCATGCCTGCTTCTGGGACCCAGCGGCCAGGTCTGCAGCAGGAGAGTTGGCGGCTGAGT TGGTGACCACCAAGCCGGAATTCACAAATTTGCAAGTATTTGATCTAGAAGGAAATGAAGCGCAACGGCA CCAGGCTAAACTTTGCTAAAGCCAACAGCACCAAGTCGGGTAGCACAAGAGCAAGGGATTTAATGAACAA AATGTCGATTATACCAGAATGGCATAGCTTGTACTCTAACACCCGGAAGCTGCCATGTTCCCGTCCCCAC TCCTCGCCATGCAAAATGCAGAGAACTATCTTCCAAGATTTATGCAGACCCTCTTCATCAACCACCGCGA TCGCCAACCCAGTTCTGTCCTACTTAGACATTGAACGGATTCTAGCTCAGAAGATTTCCAGCAGAAGGGA TGACATAAAGAAAGTCTTCCAGATACTCGACCGGAACCACAACCAGATGGTGACGAAGGGAGATCTGAAG AGAGTCATCACGGCTTTCCTCATTCCGCTCACCAAAGATCAATTCCAGGACCTGCTGGCCCAGATCCCGA TCAGCAGCTTGGGTAACGTGCCGTACCTTGAATTCCTCTCCAGGTTTGGTGGTGGAATTGATATAAACAT CAATGGTATAAAGAGGGTAAATGAAAACGAGGTAGATAACAGACGAACAGTCAAAGAAGTCCAACTCACT GAAAAGATCTTCCGGAACATGAGGAGCATCAGGAAAGTCTTCCAGGTCATGGATGTCAACAACACAGGCC TGGTGCAGCCCCAGGAGCTGAGGCGCGTTCTGGAGACATTCTGCCTGCGGATGCAGGATGGTGATTATGA GAAGTTCTTGGAACAGTACAACATCGACAAAACCACTGCAGTGGATTACAATGCTTTCCTGAAGAATCTC AGTGTTAAAAATGATGCCAGCTTTAAATACCTACTGTCCAATGCGGCAGAACTCTCAAGGGAGACTCAAC AAGGCAAAAATGGCAAGAGGCTGCTCGACACCGGGTCGTCTGAGGATGTCTGGAAGAACTACTCCTTAGA TGACCTGGAGAAGACCTTTTGCCAAGAGTTTTCGAAGTCGTATGAAAAGATCGAGAAAGCCCTCAGCGCA GGAGACCCCTCCAAAGGTGGCTACATCTCTCTGAATTACCTGAAGGTTGTCCTTGATACCTTCATATACA GGCTACCGAGGAGAATTTTTATCCAGTTAATAAGAAGATTTGGACTTAAAACCAGCACGAAAATCAACTG GAAGCAGTTTCTTACAGCAATTTATGAGCGGCAGAAGTTGGAAGTCAGTAAGACACTTCCCCTGAAAAAA AGAAGCAGCACTGAAGCTAGAAACCGGTCTCGCAAGGAAAATATCATCAAGAAGTTATTCAAGTACTCAG AGGATCGCTATACAGCCCTGAAGAAGACCTTATTAATTATCAGTTCGACGCCCAGCGGACACATAACTTG GGAAGAACTGCGGCACATTCTAAACTGCATGGTTGCGAAGTTAAACGACTCAGAATTCAGTGAGCTGAAG CAGACGTTTGACCCCGAGGGCACTGGAGCAGTGAGGGTCAACTCCCTGTTGGATGTACTGGATGACAGCA CTAAGGTGAGAAAAATGTCCCCTTCTACAGACACCAAGACACCTCTGCCTGTGGCTTGGGATTCAGTAGA GGAATTGGTGCTGGACAGCATCACCAGGAACCTCCAGGCCTTTTACAGCATGCTGCAGTCGTACGACCTT AGGGACACGGGGACCATAGGGAAAAATAACTTCAGGAAAGTCATGCGTGTATTCTGCCCATATCTATCAA ATGAGCACTTAGTAAGATTCTCCAGTAAGTTCCAAGAAGCCGGCTCAGGAAGGATACTGTACAAGAAATT TCTGTTATCCATTGGTGTGGGTATCCCACCACCTACACCCCCGCTGTCTTCTCCGAAGGTTCAGCTAAGT GACCAGTTTCAAATTGAGGAGCCGGGCCAGCAGGATGAGAGAACCCAGCCCTCCGGGAAAAAAACTTCGG AGATCAACAACATGACCAAGGAAGAAGTCATTGACGGTCTCAAGCACCGGATCCAGCAGAAGGACCCAGT ATTCAGAAAGCAGTTTCTTAGCATCAGCAAGGAACCTGATGTAAAGATCAACCAAGAGGAATTCCGGAAG GTCCTAGAGAGAAGTGGAATGCCTATGAATGACTGCCAGTATGCGATGCTGGCTTCCAAGTTAGGCTTCA AGAACGAAGAAGGCATGAGCTATCAGGACTTCACCATGGGATTTGAAGACTGTATGCTGAGCGGACTGGA AACTGTCCCATTGCAGTCCCGCACGGCCTCAAGAACGAACATGGATGAGCACTTCATATCTGCCGAGGAA TGCCTGAGAATTTTCCCTAAGAAACTGAAGGAATCCTTCCGGGACGTGTACTCCGCCTTCTTTAGAATAG ACTTGGACAGGGATGGCATTATAAGCATGCACGACTTTCACAGACTGCTGCAGTACCTACAGCTCAACAT GGTCGACCTCGAGTTTGAGCGCTTCCTCAGCCTCCTGGGCCTGAGACTTAGTGTCACTCTAAACTTCCGA GAGTTCCAGAACCTGTGTGAGAAGAGGCCCTGGAAATCAGATGAGGCACCTCAAAGGCTCATCAGATGTA AACAGAAAGTTGCAGATTCAGAGCTAGCCTGTGAGCAGGCTCATCAGTACCTCATCATGAAAGCCAAAAC CAGATGGGCAGACCTGTCCAAGAATTTCATAGAAACGGATAATGAGGGCAACGGCATCCTCCGGCGAAGG GACATCAAGAACTCGCTCTATGGCTTTGACATCCCCCTCACCCCAAGAGAATTTGAGAAGCTTTGGCAAA ACTATGACACCGAGGGAAGGGGTTATATCACGTACCAAGAGTTCCTGCACAGACTGGGCATTCGATACTC CCCGAAGGTCCACCGGCCCTACAAGGAAGACTACTTCAACTTCCTGGGTCACTTCACGAAGCCCAAGCAG GTCCAGGAGGAAATCCAGGAGCTACAGCAGATCAGTGAGAGGGAGAAACTCATGAACCATTATGAGGAGA TCAGCAAGGCGTTCAACGCAATGGAAAAATCCAAGCCCGTCGCCCTTTGCAGGGTGCAGAAGGTGCTACA GGAATGTGGGTGTCCCTTGAAGGAGGAGGAGCTCATCAGCCTTCTGAAGAGCTTGGACGTCAGTGTGCAT AACAATCACATCGACCCTGTCGAATTCCTGAGAGCGCTTGAGATCAGCTGGGCCAGCAAAGCTCGCCCAA AGGAGAAGGAGGAAAGCTCGCCACCGCCCATCAGCTTCTCTAAGGTGACCCCAGATGAGGTGATAAAGAC CATGCAGGAGGTGGTGGAGTCCTCCCAGCCTGCTTTGGTGGAGGCCTTCTCTGCACTGGATAAAGAGGAC ACAGGGTTCGTGAAGGCTATGGAATTTGGAGATGTCCTGCGGAGCGTATGCCAAAAGCTAACGGATAACC AATATCATTACTTCTTGAGGAGATTAAGGCTCCACCTAACACCCAACATACACTGGAAATACTTCCTGGA GAACTTCAGCACCTTCCAGGACGAGACTGCTGACGACTGGGCAGAGAACATGCCCAAGGCCCCGCCCCCC ATGTCCCCCAAAGAAACAGCCCACAGAGACATCGTGGCACGAGTGCAGAAGGCAGTAGCGTCTCACTACC ACACGATTGTCCAGGAGTTTGAGAACTTCGACACCCTGAAAAGCAACACGGTTTCCAGAGACGAGTTCAG GTCCATCTGCACCCGCCACATACAGATCCTGACAGACGAGCAGTTTGACAGACTGTGGAGCGAGCTGCCT GTCAATGCCAAGGGGAGGCTAAAGTACCAGGACTTCCTCAGCAAGTTGAGCATCGAGAGGGTGCCCTCGC CGCCCATGGCCGCAGGCGACTCTGGAGAATCGACCATGGCCCAGCGGGGAAGCAGCGCCCCTGAGTTCTC CCAAGGGACCAGGTCCAACCTCTACTCGCCACCTCGGGACTCCAGAGTGGGGTTGAAATCACGGAGCCAC CCCTGTACCCCAGTGGGCACCCCACCCCTACAGAACTGCGAGCCCATCGAGAGCCGGCTCCGCAAACAGA TCCAGGGCTGCTGGCGGGAGCTGCTGAGGGAGTGCAAGGAGAAAGACACGGACAAGCAGGGGACCATCTC TGCAGCTGAGTTCCTGGCGCTCGTGGAGAAGTTCAAGCTGGACATCAGCAGGGAGGAGAGCCAGCAGCTC ATCGTCAAGTACGACCTGAAGAACAACGGCAAGTTCGCCTACTGCGACTTCATCCAGAGCTGCGTCCTGC TGCTGAAGGCCAAGGAGACCTCTCTGATGCGGAGGATGAGGATCCAGAATGCGGACAAGATGAAAGAAGC TGGCATGGAGACACCTTCCTTTTACTCGGCCTTGCTACGGATCCAGCCTAAGATCGTTCACTGCTGGAGG CCCATGCGGCGTTCCTTCAAGACCTACGACAAGAACGGAACGGGACTCCTGAGTGTGGCTGACTTCAGGA AGGTGCTGCGGCAGTACAGCATTAATCTGTCAGAGGAGGAGTTCTTCCACGTGCTCGAGTACTATGACAA ATCACTGTCTTCGAAGATTTCCTACAATGACTTCCTGCGTGCCTTCCTGCAGTAGGCATCCAGGCGGAGC AGCACTTGGGACAGATGGGGCATCAGGGTCTGTCCCCAAGACTAGTCAATACCAGACAATGGAGGGCTTT AGTGAATTCCCCCAGAGCTGCTTCAGAACCTTACCCTACCCATCACGAGACTCTCAGGATACGCAGGCTC CTGCTCTAACCCTCACTGAGGCCTCCCTGGGCAACCCCTCACTGAGGCTTCCCTGGGCAACCACAGGGCG GCCGCTGGCCTCGGGCGCTCTCAATCATTTGAGCAAATAAAAATAGTCTTTGTGGGGAATGACTCTTGAG ACTTTAAAAGAACTATGACTAATAAAATGTTTTAAAACTTGGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30536704 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30536704 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18263 same embryo
	EMAGE:18258 same embryo
	EMAGE:18259 same embryo
	EMAGE:18260 same embryo
	EMAGE:18261 same embryo
	EurExpress:euxassay_012039 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source