EMAGE mouse gene expression spatial database

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EMAGE:18266

Fastkd1 FAST kinase domains 1 ( MGI:2444596)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012049. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012049_01	euxassay_012049_02	euxassay_012049_03	euxassay_012049_04

euxassay_012049_05	euxassay_012049_06	euxassay_012049_07	euxassay_012049_08	euxassay_012049_09

euxassay_012049_10	euxassay_012049_11	euxassay_012049_12	euxassay_012049_13	euxassay_012049_14

euxassay_012049_15	euxassay_012049_16	euxassay_012049_17	euxassay_012049_18	euxassay_012049_19

euxassay_012049_20	euxassay_012049_21	euxassay_012049_22	euxassay_012049_23	euxassay_012049_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18266_wholemount_strong.wlz

18266_wholemount_moderate.wlz

18266_wholemount_weak.wlz

18266_wholemount_possible.wlz

18266_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18266_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9564
Entity Detected:	Fastkd1, FAST kinase domains 1 ( MGI:2444596)
Sequence:	sense strand is shown >T9564 GCAAATAAAGCACGTGAATGAAAGCCAGATTTTATATTGTCACATTATACCTGACTTCTAGTGTAGATGC TTTTTTTTTTTAACCAAAGAAAAAATTGTGCTAAGGAAAAGAAACCTCTATTTTGGAGCCCCGTCCGTTA TAAATATGTTCCGGCTACGAAGCATCTCTTTACTCTCTTGGAGAGCATTTCCGCTCCGCCCCTTCAGTTG TGAGTCATTAATTACTCAGATGCAGAAGTGTACAAACGAAGAACAAGTGTTTGACCTTATTGAGACGAAT ACGGCAACGCTCTCCGAACAGCAAGTGGGCTGTGCGTTTAATGTACTTTGGCAGTTTCAGAAGCAGAAGA CCGTCTTGGAAAAAAACGTTGACCATGTTAGAAACCATCCTCAGTTTCTTACTCTTTGCAGCATTACAAC AAATCACATCCCAGCAATGAGTGATGCTACCCTGGTGGATGTGTTATACAGCATAAAGCAGTTTGCAGTT GAATCCCATCACCCACTAATCGAAGCACTGGTTACAGAAGCATGGAAAAGACTGGAAAGGTTCGATACTA ATGTGCTGTCCATATTTTCCACTTGCTTGGCCGATCAGCATCTGTATTTCAGTCCATTAATGGGAAAAAT AGCTGATATTGTAAATAGGAGACTGGAAACCATCCAGGACTTAAGGGCCTTATCTGTCTTAATGGTCAGC ATTTCTTCATTAATATCACCGTGTTTCCAGGAACGACTGGTGATCAGAACAGAACTTCTTTTTGACACTG TAAATTCTTCCAAAGTCAACATTGCAAGAAGGATATTACTGTTTCTTCGAAATGTCAAATACAGTCATTA CCCACTGTTAGAGAGGTGTAATCAAGTGTTTATAAGGAACATGAGCCATCTTGATCTGGAGTCCATCAGT AAAATCCTTAACCTGTATCAGTTTCTACAGTTCCACAGCTTTGAATTTGTTGAAGCAGCTCGAGGGAGGC TGGCTGAGATGACCCTCCCTTCTGATCATCCTGAAAGCTTTGTCCGGTTGTTTGCCGCTCTGGGGCCGGT GGCCAGACCTGAGATAAAGAAACAGCTTAAATCAACTATACTACTGCTGTCAGAGGAGCTGAGCAGCCAG CAAGCGCTGATCGTGTTGGGAGCAATGGAAGATATGGAAAGCAGAAATTCACACTTGCTTAAAAAGATTG TTTCTGTTCTGTACAAACATTTGGATAACTATAAATCGATAGAGCTATTGAAGATAATACAAGCATTGAC TTTTCTACATTTTCAAAGTAAGGAGCTTTTTATGAAACTCAGAGAATTACTTCTCAGTCGTTTGGAAGCT AGTGTCATACCCAGTGAGATTTCTGTTCTGGTCTCTGCTCTGTCCATGCTTCCACATCCGCACCTCAGTG AAACAGCGGTCTCCCGGATTGAAGCAGTTCTACCACAGTGTGACTTCAGGGAACTGAATGACTTGGTAGT ATATCTCATGCGCTGGATTCAGAGTGATCTCGTCTGCCTGGCTAGTACTACTGGAAAGCAACTGGACCTC CTTCAAAAACTAGATCAACTGGGCCGTCACCGGCTGCAACAGAGCACCAACTTGGATCTTCTGTGGGAGG AGCTCAAGTCTTTGAAGGGAGAATGGCTTCACGAATCCTTAGTTGAAGAATCCATCGCTGCTTTACTGCG TTTCATGGATGAAATAGATTACAGCAATATTGCCAAGGTCGCGTCTTTTCTTTCCAGAACTAACTACCTC AACACTCTGCTGCTGGATAGGATCGCTTCAGTGGCCATTCAGCAGGTTGAAAAGATCCATCCCTTTTCAG TCCTTGCTATTATTCTCCCATTCAGCATTCTGAACTATGATCCACCTCAAAAGGATGAATTCTTTGGAGC GTGTGTCCAATGCTGTAATTCTTACTTAGGTACATTGGATCCTGGTACATTAGTGTTTCTTGGTTTTTCT TTGGCCGTACTTGAATATTTTCCAGAAGATCTGCTAAAGAAAATGTTTAACATCGAGTTCCTTGCCAGAT TAGATTCCCAGCTTGAAATTTTACCTTCAAGTCTGAGTGCAAGGATCCAGTTCCGCCTCATGGAGTTAAA TAGAGCAGTCTGCTTGGAGTGCCCCGAGCTGCAGGTTCCATGGTTTCATGACCGCTTTTGTCAGCGACAG TTTAACAAAGACACTGGTGTTATGAACGGAGCCCAACAACAGATCTATAAAATGTTAGCAGAGGTCCTGG GAGGACACCAATGTGTGAAGCCTTCCGCTCTTTCACCGTATTACCACACAGTGGGTTTTGAATGCATTTT GGATAAACGAAAAAAACCTCTGCCTTATGAAAGCCATAGCATAGCTCCCAGAAAATCCCTGGGCATGCAC TGGGATTCAAGAGTTGAGCCAAGGCTTCCACCAGAAGCTGAGAGGATTGCTATAGAACTTTTGGATGTAA GAGCCTTTTGTTCAAACATTCCTCACTTAAAAGGAAAATCTGCTATGAAAAAGCGGCATTTGGAAATCTT GGGCTACCGTGTTATTCAGATCCCTTACTTTGAGTGGAACTCTATGGCAATGTCAACGAAGGATGCACGG ATGGACTACCTGAGAGAACATCTATTTGGGGAAGGCAAATCATGACTGTAGTTTTCATTTAAAATGACTG TGGTGGACTGGAGAGGTGGCACAGAGGAAAAGAACACTGGCTGCTCTTGCAGAGATCCTAAGTTCAATCC CCAGCACCCACATGGCGGCTCATAACCATCTATAATGTGATCTGGGACCCTCTTCTGGCCTGCAGGCATA CATGCAGGCAGAACACTGTATACATAATAAATAAATAAATAAATAAATCTTTTTAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30609755 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30609755 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18265 same embryo
	EMAGE:18267 same embryo
	EMAGE:18264 same embryo
	EurExpress:euxassay_012049 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source