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EMAGE:18341

Pik3c3 phosphoinositide-3-kinase, class 3 ( MGI:2445019)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18341 EMAGE:18341 EMAGE:18341 EMAGE:18341 EMAGE:18341
"Pseudo-wholemount" of euxassay_014288. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014288_01 euxassay_014288_02 euxassay_014288_03 euxassay_014288_04
EMAGE:18341 EMAGE:18341 EMAGE:18341 EMAGE:18341 EMAGE:18341
euxassay_014288_05 euxassay_014288_06 euxassay_014288_07 euxassay_014288_08 euxassay_014288_09
EMAGE:18341 EMAGE:18341 EMAGE:18341 EMAGE:18341 EMAGE:18341
euxassay_014288_10 euxassay_014288_11 euxassay_014288_12 euxassay_014288_13 euxassay_014288_14
EMAGE:18341 EMAGE:18341 EMAGE:18341 EMAGE:18341 EMAGE:18341
euxassay_014288_15 euxassay_014288_16 euxassay_014288_17 euxassay_014288_18 euxassay_014288_19
EMAGE:18341 EMAGE:18341 EMAGE:18341 EMAGE:18341 EMAGE:18341
euxassay_014288_20 euxassay_014288_21 euxassay_014288_22 euxassay_014288_23 euxassay_014288_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18341Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18341_wholemount_strong.wlz
18341_wholemount_moderate.wlz
18341_wholemount_weak.wlz
18341_wholemount_possible.wlz
18341_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18341_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 09 10 15 17 moderate expression: see section 01 02 03 04 05 06 07 08 16 18 19 20 21 22 23 weak expression: see section 24
humerus
strong strong
regionalstrong expression: see section 03 04 21 22 23 24 moderate expression: see section 01 02 weak expression: see section 05 20
fibula
moderate moderate
regionalmoderate expression: see section 23 24
tibia
moderate moderate
regionalmoderate expression: see section 23 24
femur
strong strong
regionalstrong expression: see section 03 04 05 21 moderate expression: see section 01 02 06 18 19 20 22 23
meckel's cartilage
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 15 16 17 18 19 20 21 moderate expression: see section 03 04 12 13 14
axial skeleton
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 17 moderate expression: see section 08 16 18
basioccipital bone
strong strong
regionalstrong expression: see section 10 11 12 13 14 moderate expression: see section 07 08 09 15 16 17
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 08 09 15 16 17 18
exoccipital bone
moderate moderate
regionalmoderate expression: see section 04 05 06 07 19 20 21 22 23
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 03 04 05 06 20 21 22 23 weak expression: see section 02
vault of skull
moderate moderate
regionalmoderate expression: see section 03 04 20 weak expression: see section 01 02
orbito-sphenoid
strong strong
regionalstrong expression: see section 11 12 13 14 moderate expression: see section 03 04 05 06 07 08 09 10 17 18 19 22 23 24 weak expression: see section 01 02
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
clavicle
moderate moderate
regionalmoderate expression: see section 06 17 weak expression: see section 07 08 16 18
scapula
strong strong
regionalstrong expression: see section 21 22 moderate expression: see section 03 04 23
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 15 moderate expression: see section 08 16 weak expression: see section 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31447
Entity Detected:Pik3c3, phosphoinositide-3-kinase, class 3 ( MGI:2445019)
Sequence:sense strand is shown

>T31447
CGATCTCAAACCCAACGCTACCACAAGAGATCAGCTAAATATTATTGTGAGTTACCCACCAACCAAGCAA
CTCACATATGAAGAACAAGATCTTGTTTGGAAATTTAGATATTATCTTACTAATCAAGAAAAAGCTCTGA
CGAAGTTCTTGAAGTGTGTTAATTGGGACCTGCCCCAGGAGGCCAAGCAGGCCCTGGAACTTCTGGGAAA
GTGGAAGCCAATGGATGTAGAGGACTCCCTGGAGCTGCTCTCCTCTCATTACACCAACCCCACCGTGAGG
CGCTACGCTGTGGCCCGCCTGAGGCAGGCCGATGATGAGGATTTGCTGATGTACTTACTGCAGCTGGTCC
AGGCTCTGAAATATGAAAACTTTGATGACATAAAGAATGGTTTGGAACCTACGAAGAAGGATAGTCAAAC
TTCAGCATCAGAAAGTCTGTCCAATTCTGGAGTCAGTTCTGGAGACATAGATAGCTCCCAAATTATAACC
AACCCTCTTCCTCCCGTGGCCCCCCCTCCTGCATCTAAAGCAAAGGAAGTTTCGGATGGGGAGAGTCTCG
AGCAAGATCTATGTACGTTCTTGATATCAAGAGCCTGTAAGAACTCAACACTGGCTAATTATTTATACTG
GTATGTGATAGTGGAGTGTGAAGATCAGGACACCCAGCAGCGGGACCCAAAGACTCACGAGATGTACCTG
AACGTGATGAGGCGCTTCAGCCAAGCCTTGCTCAAGGGTGACAAGTCTGTGAGAGTCATGCGCTCCCTGC
TGGCTGCTCAGCAGACCTTTGTAGATCGCCTGGTCCATCTGATGAAGGCAGTGCAGAGAGAAAGTGGGAA
CCGCAAGAAGAAGAATGAGAGACTTCAGGCCTTGCTTGGCGATAATGAGAAAATGAACTTATCAGATGTG
GAACTGATCCCATTGCCGCTGGAGCCACAGGTGAAAATAAGGGGCATCATCCCGGAAACAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3661094), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 25456. Forward Primer - name:025456_F_IRAV59-62_I19_Pik3c3, sequence:CGATCTCAAACCCAACGC; Reverse Primer - name:025456_R_SP6_IRAV59-62_I19_Pik3c3, sequence:GCTGTTTCCGGGATGATG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18338 same embryo
 EMAGE:18342 same embryo
 EMAGE:18337 same embryo
 EMAGE:18340 same embryo
 EMAGE:18339 same embryo
 EurExpress:euxassay_014288 same experiment
 MGI:4827206 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS