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EMAGE:18380

Kpna6 karyopherin (importin) alpha 6 ( MGI:1100836)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18380 EMAGE:18380 EMAGE:18380 EMAGE:18380 EMAGE:18380
euxassay_014315_01 euxassay_014315_02 euxassay_014315_03 euxassay_014315_04 euxassay_014315_05
EMAGE:18380 EMAGE:18380 EMAGE:18380 EMAGE:18380 EMAGE:18380
euxassay_014315_06 euxassay_014315_07 euxassay_014315_08 euxassay_014315_09 euxassay_014315_10
EMAGE:18380 EMAGE:18380 EMAGE:18380 EMAGE:18380 EMAGE:18380
euxassay_014315_11 euxassay_014315_12 euxassay_014315_13 euxassay_014315_14 euxassay_014315_15
EMAGE:18380 EMAGE:18380 EMAGE:18380 EMAGE:18380 EMAGE:18380
euxassay_014315_16 euxassay_014315_17 euxassay_014315_18 euxassay_014315_19 euxassay_014315_20
EMAGE:18380 EMAGE:18380 EMAGE:18380
euxassay_014315_21 euxassay_014315_22 euxassay_014315_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18380Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18380_wholemount_strong.wlz
18380_wholemount_moderate.wlz
18380_wholemount_weak.wlz
18380_wholemount_possible.wlz
18380_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18380_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30762
Entity Detected:Kpna6, karyopherin (importin) alpha 6 ( MGI:1100836)
Sequence:sense strand is shown

>T30762
GGATGACGACAGCAGCCTGGCTCCTCAGGTGGATGAGACGCAACAGCAGTTCATCTTCCAGCAGCCTGAG
GCCCCCATGGAGGGCTTCCAGCTATAATGTCTGCCTCCGGGGAGGGGAGGGCACGGGAAGCACCAGCAGC
CAGCAGAGAGAGGAGCAGCCTTCTGGTGGGCAGGAGAGCAGCCACCCCACCAGCAGCCCCGCACACCTGC
TGCCCCGAGACTGTGCTCTCGACCTGTTCATCCCCTTCCCTGGAGGGAGCAAGCGCCCTCTGGACAGACA
GAACCATCTGAGGCTCACATTGGGTTTTGAGACAAGAAGGGGACGTGTGGGTTTCTCTTCCTTACACTAT
TTTGGCTGCACACGTCTTTAACCCAGGAGCCCAGGGGTAGACAAAGGAGGACTGAGGTAATCAATTTGCA
CCTTTAAAATTTTTTTCTTTTATTTTCTTTAGTGGTGACTTCCTTCCCAATTATCTTTCTTCATCCATCC
CAGTCCTCTGCCCTGACGTGTGTAACTCTTGGGTACTTGAGCAGATGGAATATTCCAGAGGTGGGAGGTG
GGAGGGGAGGGGAGAAGTCCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2651272), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 12349. Forward Primer - name:012349_F_IRAV01-03_K23_Kpna6, sequence:GGATGACGACAGCAGCCT; Reverse Primer - name:012349_R_SP6_IRAV01-03_K23_Kpna6, sequence:TTGGGACTTCTCCCCTCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18385 same embryo
 EMAGE:18381 same embryo
 EMAGE:18384 same embryo
 EMAGE:18383 same embryo
 EMAGE:18382 same embryo
 EurExpress:euxassay_014315 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS