EMAGE mouse gene expression spatial database

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EMAGE:18386

Capg capping protein (actin filament), gelsolin-like ( MGI:1098259)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014310. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014310_01	euxassay_014310_02	euxassay_014310_03	euxassay_014310_04

euxassay_014310_05	euxassay_014310_06	euxassay_014310_07	euxassay_014310_08	euxassay_014310_09

euxassay_014310_10	euxassay_014310_11	euxassay_014310_12	euxassay_014310_13	euxassay_014310_14

euxassay_014310_15	euxassay_014310_16	euxassay_014310_17	euxassay_014310_18	euxassay_014310_19

euxassay_014310_20	euxassay_014310_21	euxassay_014310_22	euxassay_014310_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18386_wholemount_strong.wlz

18386_wholemount_moderate.wlz

18386_wholemount_weak.wlz

18386_wholemount_possible.wlz

18386_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18386_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 21 22 23

cornea epithelium

strong

regional

strong expression: see section 02 03 22 23

anterior naris epithelium

strong

regional

strong expression: see section 12 13 16 17

tongue epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

mandible

strong

single cell

strong expression: see section 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21

lower jaw incisor

strong

regional

strong expression: see section 10 11 12 15 16

lower jaw molar

strong

regional

strong expression: see section 07 17 18

oral epithelium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

maxilla

strong

single cell

strong expression: see section 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22

upper jaw incisor

strong

regional

strong expression: see section 11 12 15 16

upper jaw molar

strong

regional

strong expression: see section 06 07 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30767
Entity Detected:	Capg, capping protein (actin filament), gelsolin-like ( MGI:1098259)
Sequence:	sense strand is shown >T30767 GGCCCATAGCACGAGAGAGCCATGGCATCTTTTTCTCTGGGGACTCCTACCTAGTGCTTCACAATGGCCC AGAGGAGGCTTCCCATCTGCACCTGTGGATAGGCCAGCAGTCCTCCCGGGATGAGCAGGGGGCCTGTGCA GTGCTGGCTGTGCATCTCAACACCCTGCTGGGGGAGCGGCCAGTGCAGCACCGTGAGTTGCAAGGCAATG AGTCTGACCTCTTCATGAGCTACTTCCCACGAGGCCTCAAGTACCGGGAAGGTGGTGGTAGAGTCGGCAT TTCACAAGACAACCTTCGCGCCACCCCAGCAGCCATCAGGAAGCTCTACCAGGTTAAGGGGAAGAAGAAC ATCCGTGCGACCGAGAGGGCTCTGAGTTGGGACAGCTTCAACACTGGGGACTGCTTCATCCTGGACCTGG GTCAGAACATCTTTGCCTGGTGTGGTGGAAAGTCCAACATCCTTGAGCGCAACAAGGCGAGGGACCTGGC CCTGGCCATCAGGGACAGCGAGCGGCAGGGCAAGGCCCAGGTGGAAATCATCACTGATGGAGAGGAGCCA GCCGAGATGATTCAGGTTCTGGGCCCCAAGCCTGCTCTGAAGGAGGGTAACCCCGAGGAAGACATTACAG CTGACCAGACCAACGCCCAGGCTGCAGCCCTGTATAAGGTCTCTGATGCCACTGGACAGATGAATCTGAC CAAGGTGGCTGACTCCAGCCCTTTTGCCTCTGAACTGCTAATTCCAGATGACTGCTTTGTTCTGGACAAC GGGCTGTGTGGCAAAATCTACATCTGGAAGGGGAGAAAAGCTAATGAGAAAGAGCGGCAGGCAGCCCTCC AAGTGGCTGATGGCTTCATCTCTCGAATGAGGTATTCCCCAAACACTCAGGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2811050), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 37602. Forward Primer - name:037602_F_IRAV01-03_M09_Capg, sequence:GGCCCATAGCACGAGAGA; Reverse Primer - name:037602_R_SP6_IRAV01-03_M09_Capg, sequence:CCACCTGAGTGTTTGGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18390 same embryo
	EMAGE:18389 same embryo
	EMAGE:18387 same embryo
	EMAGE:18388 same embryo
	EurExpress:euxassay_014310 same experiment
	MGI:4823614 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source