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EMAGE:18436

Phkg1 phosphorylase kinase gamma 1 ( MGI:97579)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18436 EMAGE:18436 EMAGE:18436 EMAGE:18436 EMAGE:18436
"Pseudo-wholemount" of euxassay_014397. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014397_01 euxassay_014397_02 euxassay_014397_03 euxassay_014397_04
EMAGE:18436 EMAGE:18436 EMAGE:18436 EMAGE:18436 EMAGE:18436
euxassay_014397_05 euxassay_014397_06 euxassay_014397_07 euxassay_014397_08 euxassay_014397_09
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euxassay_014397_10 euxassay_014397_11 euxassay_014397_12 euxassay_014397_13 euxassay_014397_14
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euxassay_014397_15 euxassay_014397_16 euxassay_014397_17 euxassay_014397_18 euxassay_014397_19
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euxassay_014397_20 euxassay_014397_21 euxassay_014397_22 euxassay_014397_23 euxassay_014397_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18436Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18436_wholemount_strong.wlz
18436_wholemount_moderate.wlz
18436_wholemount_weak.wlz
18436_wholemount_possible.wlz
18436_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18436_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31832
Entity Detected:Phkg1, phosphorylase kinase gamma 1 ( MGI:97579)
Sequence:sense strand is shown

>T31832
TCCTGCAGAAGGTCTCGGGACACCCCAACATCATACAGCTGAAGGACACTTACGAGACCAACACTTTCTT
CTTCTTGGTATTTGATCTGATGAAGAGAGGGGAACTCTTTGACTATCTCACTGAGAAGGTCACCTTAACC
GAGAAGGAAACCAGAAAGATCATGCGGGCCCTGCTGGAGGTGATCTGTACCCTGCACAAACTCAACATTG
TCCATCGGGACCTGAAGCCGGAGAATATCCTTTTGGATGACAACATGAATATAAAGCTCACAGACTTCGG
GTTTTCCTGCCAGCTGCAGCCAGGAGAGAAGCTCCGAGAGGTTTGTGGGACTCCCAGTTATCTGGCCCCT
GAAATCATACAGTGCTCCATGGACGACGGCCATCCTGGCTATGGGAAGGAGGTGGACATGTGGAGCACAG
GCGTCATCATGTACACTCTGCTGGCTGGCTCCCCGCCTTTCTGGCACCGGAAGCAAATGCTGATGTTGCG
GATGATCATGGACGGCAAATACCAGTTTGGCTCACCAGAGTGGGATGACTACTCTGACACAGTGAAAGAC
TTGGTGTCTCGATTCTTGGTGGTGCAACCTCAGGACCGCTGCTCGGCGGAAGAGGCCTTGGCACACCCTT
TCTTTCAGGAGTACGTAGTAGAAGAAGTACGGCACTTCAGCCCCCGAGGGAAGTTCAAGGTGATCTGTCT
AACTGTGCTGGCATCAGTAAAGATCTACTACCAGTACCGTCGGGTGAAGCCGGTAACCAGGGAGATCGTC
ATCCGAGACCCCTACGCCCTTCGGCCGCTGCGGAGGCTCATTGACGCCTATGCTTTCCGTATCTACGGCC
ACTGGGTGAAGAAAGGGCAACAGCAGAACAGAGCCGCCCTCTTCGAGAACACGCCCAAGGCTGTGCTCCT
CTCCTTGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6532722), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60979. Forward Primer - name:060979_F_IRAW1_d03_Phkg1, sequence:TCCTGCAGAAGGTCTCGG; Reverse Primer - name:060979_R_SP6_IRAW1_d03_Phkg1, sequence:GCCAAGGAGAGGAGCACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18437 same embryo
 EMAGE:18439 same embryo
 EMAGE:18438 same embryo
 EMAGE:18440 same embryo
 EurExpress:euxassay_014397 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS