EMAGE mouse gene expression spatial database

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EMAGE:18523

Rg9mtd2 RNA (guanine-9-) methyltransferase domain containing 2 ( MGI:1920421)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014471. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014471_01	euxassay_014471_02	euxassay_014471_03	euxassay_014471_04

euxassay_014471_05	euxassay_014471_06	euxassay_014471_07	euxassay_014471_08	euxassay_014471_09

euxassay_014471_10	euxassay_014471_11	euxassay_014471_12	euxassay_014471_13	euxassay_014471_14

euxassay_014471_15	euxassay_014471_16	euxassay_014471_17	euxassay_014471_18	euxassay_014471_19

euxassay_014471_20	euxassay_014471_21	euxassay_014471_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18523_wholemount_strong.wlz

18523_wholemount_moderate.wlz

18523_wholemount_weak.wlz

18523_wholemount_possible.wlz

18523_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18523_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

weak

regional

weak expression: see section 02 03 18 19

femur

weak

regional

weak expression: see section 02 03 04 05 06 18 19 20

ventral grey horn

weak

regional

weak expression: see section 09 11 13 14 15

meckel's cartilage

moderate

regional

moderate expression: see section 09 10 11 13 14 15 16 weak expression: see section 04 05 06 07 08 18

axial skeleton

weak

regional

weak expression: see section 09 10 11 12 13 14 15

basioccipital bone

weak

regional

weak expression: see section 11 12 13 14

basisphenoid bone

weak

regional

weak expression: see section 11 12 13 14

exoccipital bone

weak

regional

weak expression: see section 03 17 18

temporal bone

weak

regional

weak expression: see section 02 03 18 20

orbito-sphenoid

weak

regional

weak expression: see section 02 03 04 05 06 07 16 17 18 19 20

scapula

weak

regional

weak expression: see section 02 03 18 19

pelvic girdle skeleton

weak

regional

weak expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37972
Entity Detected:	Rg9mtd2, RNA (guanine-9-) methyltransferase domain containing 2 ( MGI:1920421)
Sequence:	sense strand is shown >T37972 ATGAGTCAAAGGCCTACGTGATTGGAGGGCTAGTGGACCACAGCCATCACAAGGGACTCACATTTAAACA AGCAACCAGTTACGGAATCGAACATGCACAGCTCCCTCTTGCAGATTTTGTGAAGATGAACAGTCGAAAA GTCCTGGCCGTTAACCATGTGTTTGAAATTATCCTGGAGTTCTTGGAAACAAGAGACTGGCAAGAGGCAT TCTTTACAATCTTACCCCAACGGAAAGGGGCCGTTCCTGCTCACAAGGCCTGCGAAAGCTCTCCTCAGGA CCACCAGTCCCTGCCAGAAGGATGGGACAGTGCCAGCGAGGGAGAGAGTTGCAGGGATAATCCTGACTCA CCACAGAAAGACGAGCAGGGCCAGCAGAGCAGCCCGGTTCTGCAGTGAGCCCCGTCCCACAGTAACCTTC CCCTGTCCTCCTCTCGCTTAAGAGAAACCAGGCAGTGCGGTGCTTTACAGAATGCTCAGACTGCAAGAAA ACTTTCTCTAGTCTATGTTGTGGAATTTTAAATCTGTTTTAAGAGTTAAATAATAATGAAAAAAACCCTT TAAACACTAAGAAAGGATCGTTTGGTTTTGTGTAAGAAGACTTAAAGATATGATTTCAAAATTGCTTTAA GCCTCAAATCAGTCATGGAGGATGTGGAATACGAGTCTGCCTCGGTTCGTCACTCACTGGAGCATATCCT TCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68582. Forward Primer - name:068582_F_cDNA_Rg9mtd2, sequence:ATGAGTCAAAGGCCTACGTGAT; Reverse Primer - name:068582_N_SP6_cDNA_Rg9mtd2, sequence:TGAAGGATATGCTCCAGTGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18525 same embryo
	EMAGE:18527 same embryo
	EMAGE:18526 same embryo
	EMAGE:18524 same embryo
	EurExpress:euxassay_014471 same experiment
	MGI:4827709 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source