EMAGE mouse gene expression spatial database

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EMAGE:18590

Als2cl ALS2 C-terminal like ( MGI:2447532)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014538. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014538_01	euxassay_014538_02	euxassay_014538_03	euxassay_014538_04

euxassay_014538_05	euxassay_014538_06	euxassay_014538_07	euxassay_014538_08	euxassay_014538_09

euxassay_014538_10	euxassay_014538_11	euxassay_014538_12	euxassay_014538_13	euxassay_014538_14

euxassay_014538_15	euxassay_014538_16	euxassay_014538_17	euxassay_014538_18	euxassay_014538_19

euxassay_014538_20	euxassay_014538_21	euxassay_014538_22	euxassay_014538_23	euxassay_014538_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18590_wholemount_strong.wlz

18590_wholemount_moderate.wlz

18590_wholemount_weak.wlz

18590_wholemount_possible.wlz

18590_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18590_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38817
Entity Detected:	Als2cl, ALS2 C-terminal like ( MGI:2447532)
Sequence:	sense strand is shown >T38817 CTCGGTCTGCTCAGTTCTCTTTCCCGTGAGGGAACCTGTTTCCTCCTCCTCAGCTGGGATTCAGTGTTGA GCTGGCCTCTCAGTAGCACCTGGGACAGGAAAACCGCAGGTCCATGCTCAGCTGCCTCGTTGCCTCTGAC AGGCCCTGCCCTTTGCAAAGGCATGTGATATAAACACCAGCAATCATTATCTGGCCTGGCTTGCCAGCAG ACGTACCTGAGACACTTTCTGACCTCTCTGTGGGAGAGACAACCGGGATATCCAAGGCTTACTAGTGCCC TGACAGACGAACATCTCTGGCTCAGGACCATGAATAAGTCTGGTCACAGCGCTGAGTTCTGGGTCACTGT TCCTGGCCCCCGGTATACTTCCCACCAATTCAGCCTGTGTGGGAAGGGCAGAAGGAGATGCTGAAAAAGG TAATGCCTAAGGCCGTGGCTGAAGCTCTTGGGAGCCTCAGAGAGGGACACCTTCAGAGCCTCTCAGAAAA TGTGGAGCCCTGTGTGAAGAGAGCTGGTGGTTCTCCTGCACTGTGAAACCCAGGAGAATGCAAAGAGGCT TTCTCTGGCTGGGTCCACCAAATATCTCATCACAAACAGAGGACCCGGGCTGGAGTTCTACCAGGAACTT GTGTCCCTAGGAAGATCCTTGGAAGGGTTAAACTGACGGAGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 235772. Forward Primer - name:164927_F_cDNA_BC022645, sequence:CTCGGTCTGCTCAGTTCTCTTT; Reverse Primer - name:164927_N_SP6_cDNA_BC022645, sequence:GCCTCCGTCAGTTTAACCCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18589 same embryo
	EMAGE:18591 same embryo
	EMAGE:18593 same embryo
	EMAGE:18592 same embryo
	EurExpress:euxassay_014538 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source