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EMAGE:18609

D17Wsu104e DNA segment, Chr 17, Wayne State University 104, expressed ( MGI:2156020)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18609 EMAGE:18609 EMAGE:18609 EMAGE:18609 EMAGE:18609
"Pseudo-wholemount" of euxassay_014582. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014582_01 euxassay_014582_02 euxassay_014582_03 euxassay_014582_04
EMAGE:18609 EMAGE:18609 EMAGE:18609 EMAGE:18609 EMAGE:18609
euxassay_014582_05 euxassay_014582_06 euxassay_014582_07 euxassay_014582_08 euxassay_014582_09
EMAGE:18609 EMAGE:18609 EMAGE:18609 EMAGE:18609 EMAGE:18609
euxassay_014582_10 euxassay_014582_11 euxassay_014582_12 euxassay_014582_13 euxassay_014582_14
EMAGE:18609 EMAGE:18609 EMAGE:18609 EMAGE:18609 EMAGE:18609
euxassay_014582_15 euxassay_014582_16 euxassay_014582_17 euxassay_014582_18 euxassay_014582_19
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euxassay_014582_20 euxassay_014582_21 euxassay_014582_22 euxassay_014582_23 euxassay_014582_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18609Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18609_wholemount_strong.wlz
18609_wholemount_moderate.wlz
18609_wholemount_weak.wlz
18609_wholemount_possible.wlz
18609_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18609_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38813
Entity Detected:D17Wsu104e, DNA segment, Chr 17, Wayne State University 104, expressed ( MGI:2156020)
Sequence:sense strand is shown

>T38813
ATTCGTTCTCCCAGGACGTAGGACCCGGGAACAAGTTTACATGTACATTCACCTACGCTTCCCAAGGAGG
GACCAACGAGCAATGGCAGATGAGCCTGGGGACAAGTGAAGACAGCCAGCACTTTACCTGTACCATCTGG
AGGCCCCAGGGGAAATCCTACCTCTACTTCACACAGTTCAAGGCTGAGTTGCGAGGTGCTGAGATCGAGT
ATGCCATGGCCTACTCCAAAGCCGCATTTGAGAGAGAGAGTGATGTCCCCCTGAAAAGTGAGGAGTTTGA
AGTGACCAAGACAGCAGTGTCTCACAGGCCTGGGGCCTTCAAAGCTGAGCTCTCCAAGCTGGTGATCGTA
GCCAAGGCGGCACGCTCGGAGCTGTGACCCTCGCCTGTCAAGGGCCTTCATGTCCACGTTCCTCAGGCAC
ACTGACCGGGACTACTTGTCTAGGGCACTGGTTCCCATAGGAGCTGCCCTGCCCTGCACAGGTCACACTG
TGTCACTCCGCAGAACTCTCTGAGCCCGGTCACCTGTTTTGCCAGGGAAGAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 236457. Forward Primer - name:165612_F_cDNA_D17Wsu104e, sequence:ATTCGTTCTCCCAGGACGTAG; Reverse Primer - name:165612_N_SP6_cDNA_D17Wsu104e, sequence:ATCTTCCCTGGCAAAACAGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18606 same embryo
 EMAGE:18605 same embryo
 EMAGE:18607 same embryo
 EMAGE:18608 same embryo
 EMAGE:18604 same embryo
 EurExpress:euxassay_014582 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS