EMAGE mouse gene expression spatial database

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EMAGE:18656

Cep110 centrosomal protein 110 ( MGI:1889576)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016548. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016548_01	euxassay_016548_02	euxassay_016548_03	euxassay_016548_04

euxassay_016548_05	euxassay_016548_06	euxassay_016548_07	euxassay_016548_08	euxassay_016548_09

euxassay_016548_10	euxassay_016548_11	euxassay_016548_12	euxassay_016548_13	euxassay_016548_14

euxassay_016548_15	euxassay_016548_16	euxassay_016548_17	euxassay_016548_18	euxassay_016548_19

euxassay_016548_20	euxassay_016548_21	euxassay_016548_22	euxassay_016548_23	euxassay_016548_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18656_wholemount_strong.wlz

18656_wholemount_moderate.wlz

18656_wholemount_weak.wlz

18656_wholemount_possible.wlz

18656_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18656_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 15 16 18 19 20 21

midbrain ventricular layer

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40556
Entity Detected:	Cep110, centrosomal protein 110 ( MGI:1889576)
Sequence:	sense strand is shown >T40556 AATTAATCAGGAGCGAGCAGAGGAGCTGCAGGAGGCAGAGAGGTTCAGCAGAGAGGCCATGCAAGCAGCC AAGGACCTGAGCAGAGCGGAGGCAGAGATCGAGCTCCTGCAGCACCTTCTCAGGGAGAGAGAGGGCCAGT TCCGAGATGAGATGGAAAATGCAGATTTAGGTGCTAAAGGAGCAAACTCCCAGCTGCTAGAGATTGAGGC GCTGAACGAGGCCATGGCGAAGCAAAGAGCTGAGATCACGAGACTCCGGGATGTATTAAACCTCACGGGG GCAGGTACAAAAGGAGGCATTGAGAACGTGTTAGAGGAAATCGCTGAGCTCCGGCATGCAGTCTCTGCTC AGAACGAGTACATCAGCAGCATGGCAGATCCTTTCAGAAGACAAGGCTGGTGGTACTTTATGCCACCAGC ACCATCCTCAAAAGTTTCCAGCCATAGTTCTCAGGCCACCAAGGACTCCGGTTTGGGTCTAAAGTACACA GCCTCAACTCCTCTTAGAAAACCACAGCCTGGACAGCAAGAAGAAAAGGACAGCAGTGGACCTCTGCCTG CTTCAGGGTACTGGGTCTACTCCCCCATCAGAAGCACGCTGCATAAGTCATTTTCAAAGAGAGAAGATGC AGACAGTGGAGGAGATAGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 162852. Forward Primer - name:162852_F_cDNA_mCG21281.2, sequence:AATTAATCAGGAGCGAGCAGAG; Reverse Primer - name:162852_N_SP6_cDNA_mCG21281.2, sequence:GGCTATCTCCTCCACTGTCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18654 same embryo
	EMAGE:18657 same embryo
	EMAGE:18655 same embryo
	EMAGE:18653 same embryo
	EMAGE:18652 same embryo
	EurExpress:euxassay_016548 same experiment
	MGI:4823811 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source