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EMAGE:18681

Arid5b AT rich interactive domain 5B (MRF1-like) ( MGI:2175912)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18681 EMAGE:18681 EMAGE:18681 EMAGE:18681 EMAGE:18681
"Pseudo-wholemount" of euxassay_016566. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016566_01 euxassay_016566_02 euxassay_016566_03 euxassay_016566_04
EMAGE:18681 EMAGE:18681 EMAGE:18681 EMAGE:18681 EMAGE:18681
euxassay_016566_05 euxassay_016566_06 euxassay_016566_07 euxassay_016566_08 euxassay_016566_09
EMAGE:18681 EMAGE:18681 EMAGE:18681 EMAGE:18681 EMAGE:18681
euxassay_016566_10 euxassay_016566_11 euxassay_016566_12 euxassay_016566_13 euxassay_016566_14
EMAGE:18681 EMAGE:18681 EMAGE:18681 EMAGE:18681 EMAGE:18681
euxassay_016566_15 euxassay_016566_16 euxassay_016566_17 euxassay_016566_18 euxassay_016566_19
EMAGE:18681 EMAGE:18681 EMAGE:18681 EMAGE:18681 EMAGE:18681
euxassay_016566_20 euxassay_016566_21 euxassay_016566_22 euxassay_016566_23 euxassay_016566_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18681Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18681_wholemount_strong.wlz
18681_wholemount_moderate.wlz
18681_wholemount_weak.wlz
18681_wholemount_possible.wlz
18681_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18681_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
olfactory cortex mantle layer
weak weak
regionalweak expression: see section 08 09 10 11 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45568
Entity Detected:Arid5b, AT rich interactive domain 5B (MRF1-like) ( MGI:2175912)
Sequence:sense strand is shown

>T45568
TCTCCCTGCTCCTGAAGTAAAGGGGAAACCAGAAGGGCACAAGGATCTTGGAGCGAGGGCCCCAGTGTCC
AGAGCAGACCCAGAAAAGGCCAATGAAACTGACCAAGGTTCCAACAGCGAGAAAGAGGCCGAGGAGATGG
GAGATAAGGGCCTTGCCCCTCTGCTTCCAAGCCCTCCCTTGCCTCCTGAAAAAGATTCAGCTCCCACCCC
TGGGGCTGGCAAACAGCCCCTTGCCTCTCCCAGCACCCAGATGGACTCAAAGCAAGAAGCCAAACCCTGC
TGCTTTACAGAGAGCCCTGAGAAGGACCTCCAAGGAGCACCCTTCTCCAGCTTCTCTGCCACCAAGCCAC
CACTGACAAGCCAGAACGAGGCAGAGGAAGAGCAGCTTCCTGCCACCGCCAACTACATTGCCAACTGCAC
TGTGAAGGTGGACCAGCTGGGCAGTGACGACATCCACACTGCTCTCAAGCAGACCCCGAAGGTCCTTGTG
GTCCAATCATTTGACATGTTCAAAGACAAAGACTTGACCGGCCCCATGAATGAGAACCACGGACTTAACT
ACACGCCCCTGCTTTACTCCCGGGGCAACCCTGGTATCATGTCCCCATTGGCTAAGAAAAAGCTTTTGTC
TCAGGTGAGTGGGGCCAGCCTCTCCAGCAGCTATCCCTATGGCTCCCCGCCCCCTTTGATCAGCAAAAAG
AAACTCATTGCCAGGGCAAGACCTGTGCTCGGGTTTGTCCCAGGGCCACCACAGCCAAAGCTCTGACCAC
ACGGCCGTCAGCCGGCCATCCGTAATCCAACATGTTCAGAGCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 173646. Forward Primer - name:173646_F_Arid5b, sequence:TCTCCCTGCTCCTGAAGTAAAG; Reverse Primer - name:173646_R_SP6_Arid5b, sequence:AGCTCTGAACATGTTGGATTACG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18679 same embryo
 EMAGE:18676 same embryo
 EMAGE:18678 same embryo
 EMAGE:18680 same embryo
 EMAGE:18677 same embryo
 EurExpress:euxassay_016566 same experiment
 MGI:4823236 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS