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EMAGE:18722

Hist2h2be histone cluster 2, H2be ( MGI:2448415)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18722 EMAGE:18722 EMAGE:18722 EMAGE:18722 EMAGE:18722
"Pseudo-wholemount" of euxassay_014003. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014003_01 euxassay_014003_02 euxassay_014003_03 euxassay_014003_04
EMAGE:18722 EMAGE:18722 EMAGE:18722 EMAGE:18722 EMAGE:18722
euxassay_014003_05 euxassay_014003_06 euxassay_014003_07 euxassay_014003_08 euxassay_014003_09
EMAGE:18722 EMAGE:18722 EMAGE:18722 EMAGE:18722 EMAGE:18722
euxassay_014003_10 euxassay_014003_11 euxassay_014003_12 euxassay_014003_13 euxassay_014003_14
EMAGE:18722 EMAGE:18722 EMAGE:18722 EMAGE:18722 EMAGE:18722
euxassay_014003_15 euxassay_014003_16 euxassay_014003_17 euxassay_014003_18 euxassay_014003_19
EMAGE:18722 EMAGE:18722 EMAGE:18722 EMAGE:18722
euxassay_014003_20 euxassay_014003_21 euxassay_014003_22 euxassay_014003_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18722Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18722_wholemount_strong.wlz
18722_wholemount_moderate.wlz
18722_wholemount_weak.wlz
18722_wholemount_possible.wlz
18722_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18722_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 12 13 14 moderate expression: see section 05 06 07 15 weak expression: see section 16
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39649
Entity Detected:Hist2h2be, histone cluster 2, H2be ( MGI:2448415)
Sequence:sense strand is shown

>T39649
AGTTTGCTGCACCCTACTTAGCTTCTTGGAGTTCTTTTTAGACTAGGATAATGGAGGTGGGTCTCTGGGC
CCGGTTTCCGTGTTCTAGGCTTTTGGGGTACCATTTACTTGTCGACTTAAAGTGAGATGGTTTCCTTACC
CTGGCGAATAGAGGGCTCCAGGACACAGCTAAGACTGTGTCGGCGAATGCTGGGTACCAGCCATCTACAA
CTGAAACCGCTCTGTCCCTTTACTTGTGTCCTGGGCACCAGCGGTTACGGGGTACTTAGGGTGGTGTCCC
TCTAATTTACTGATTTACAAACACGAATGTTCCACGTTGCTGCCGATTTGGTAGAAGTCGGTTATGGTTC
TCTATCAGGCTTTAAAATCCCTGTTCGAGGCTTGCTTGGTTTGTTTTGTTCTGTTTTTGTTTTTCCTTTT
AATTCTTTGCTTTAACCTCTGCAGTGCCAGATTACCGCAGGCTACTGCTGCTTCTCTGGGCTCATGAAGC
GATCTTTCGAATCCACACACGCAGGAATTAGCTTTTTCCCTCCTGTCGTGTCTCCAGGAGAAGTTGTCTC
AAGTTCCCTTTGCTTTCTTATTTACACTGTTGCTGATCAAGCTGGGTCTGACAGTTTTATACCTCCAGAG
CCCACCAGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68912. Forward Primer - name:068912_F_cDNA_Hist2h2bb, sequence:AGTTTGCTGCACCCTACTTAGC; Reverse Primer - name:068912_N_SP6_cDNA_Hist2h2bb, sequence:ACTGGTGGGCTCTGGAGGTAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18720 same embryo
 EMAGE:18721 same embryo
 EMAGE:18719 same embryo
 EMAGE:18723 same embryo
 EMAGE:18718 same embryo
 EurExpress:euxassay_014003 same experiment
 MGI:4825380 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS