EMAGE mouse gene expression spatial database

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EMAGE:18751

Muc16 mucin 16 ( MGI:1920982)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014145. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014145_01	euxassay_014145_02	euxassay_014145_03	euxassay_014145_04

euxassay_014145_05	euxassay_014145_06	euxassay_014145_07	euxassay_014145_08	euxassay_014145_09

euxassay_014145_10	euxassay_014145_11	euxassay_014145_12	euxassay_014145_13	euxassay_014145_14

euxassay_014145_15	euxassay_014145_16	euxassay_014145_17	euxassay_014145_18	euxassay_014145_19

euxassay_014145_20	euxassay_014145_21	euxassay_014145_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18751_wholemount_strong.wlz

18751_wholemount_moderate.wlz

18751_wholemount_weak.wlz

18751_wholemount_possible.wlz

18751_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18751_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pleura

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

stomach mesentery

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

duodenum caudal part mesentery

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

jejunum mesentery

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

midgut loop mesentery

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21

rest of midgut mesentery

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

urogenital mesentery

moderate

regional

moderate expression: see section 02 03 04 05 06 11 12 13 14 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40281
Entity Detected:	Muc16, mucin 16 ( MGI:1920982)
Sequence:	sense strand is shown >T40281 TCCATGGGCTGTACCTAGAGATTAACAATCTAACTCATGGAATCACCCAGCTGGGCAACTACTCACTGGA CAATGGTAGTCTATATATCAATGGTTATAATGAACATGGTGTAGAAGAACTTTATACAGCTATGGGGAAT TTGAAGACTTTCACACTCAATTTCACCATCTCCAACCTCCCATACTCAACAGACATGAGTAGTGGCTCAG CCATGTTCAATTCCACTGAAAGGATACTGCAGCACCTGATTAGACCCTTGGTCCAGAATGAATCCCTTTA CTCAAATTGCAGACTGGCTTCCCTCAGGCCTAAGAAGAATGGAACAGCCACTGGGGTGAATGCTATTTGC TCCTATCACCAAAACCCAGACCATCCTGAGCTTGACACTCAGGAGCTGTATACAAAGCTGACCCAGCTGA CCCAAGGAGTCACCCAGCTGGGCAGCTACATGTTGGACCAGAATAGTATCTATGTCAATGACCTGTACAA AGAACTAAGGGTCTTTTCTTCCTCTGATACTGTAGGCTATGTGCCACTGAATATAACCATACAGGGCAAG TACCAGCTAAACTTCTGCATCATCAACTGGAACCTTAACAACACAGACCCAACATCTTCAGAATATATTA CCCTAGAGAGGGACATCGAAGACAAGGTCACCACACTCTACACAGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81758. Forward Primer - name:081758_F_cDNA_1110008I14Rik, sequence:TCCATGGGCTGTACCTAGAGAT; Reverse Primer - name:081758_N_SP6_cDNA_1110008I14Rik, sequence:GCCTGTGTAGAGTGTGGTGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18752 same embryo
	EMAGE:18753 same embryo
	EMAGE:18750 same embryo
	EMAGE:18754 same embryo
	EMAGE:18749 same embryo
	EurExpress:euxassay_014145 same experiment
	MGI:4826507 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source