EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18880

9330159H11Rik RIKEN cDNA 9330159H11 gene ( MGI:2444237)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015815. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015815_01	euxassay_015815_02	euxassay_015815_03	euxassay_015815_04

euxassay_015815_05	euxassay_015815_06	euxassay_015815_07	euxassay_015815_08	euxassay_015815_09

euxassay_015815_10	euxassay_015815_11	euxassay_015815_12	euxassay_015815_13	euxassay_015815_14

euxassay_015815_15	euxassay_015815_16	euxassay_015815_17	euxassay_015815_18	euxassay_015815_19

euxassay_015815_20	euxassay_015815_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18880_wholemount_strong.wlz

18880_wholemount_moderate.wlz

18880_wholemount_weak.wlz

18880_wholemount_possible.wlz

18880_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18880_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata floor plate

weak

regional

weak expression: see section 11 12

metencephalon floor plate

weak

regional

weak expression: see section 12

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 17

trigeminal v ganglion

weak

regional

weak expression: see section 16 17 18 19 20

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40500
Entity Detected:	9330159H11Rik, RIKEN cDNA 9330159H11 gene ( MGI:2444237)
Sequence:	sense strand is shown >T40500 TTCAGAAAAGGGCAAGACTCTCAGGGATATTAGCAAACATGGCATATCAAGTTGCAATTACATTAGGCCC CTCCCCTCATAGTAAGGCTGGCAGGAGGAGGATAAAGGTCCCAAAAGCAGGCAAAAGTGTCAGGATTCCT TGAACTCTACCTAATGTTAAGCACCGGATCCCTGTATCTTTTTCCATCAGCTGCTGGATGTAGCCTCTAT GATGACAACTGGACTATGTACCAAGCTTTGAATACAGCATACAGTGTCAGGATGAAGTTTCCACACAACT TATATGTCACCTTTACCACTGATTGCTAACAGAATGGATTGTAGGTGAAAGGTTTTGTGGCTGGGTTGGT GTCCCAAACTCTTCACTAGGAATCTTGCTTCGTTATAGGAGAAAACAGAGGTTTTGTTGTTGTTGCTGCT GTTTTTTTTTTAATTATTGAATAAACATTTATACACAGATGTGAACATACTGAAAATTACATTTAGAAGA AATATATAATCTGGTTTTTTGTTCTTCAATATCATGGGTAAGTGAGACAGAATTAGAAATTTGCTGCAAA GTTATTTTCACAGTTTTTTATTGTGCCTGAAAGTATTTATCTATCTATCTAAATCCATAACAGGGTCAAA AATTCAGGAATTGGCCACATTAAGGATGTAAAAACTCATTAGTTCATAAATTGACCAGATTCCACAGGAA GCAGTCAGAGAGAAATCCTCTGTCTTACCAGTATGAACTGTTGCAGGTATTTCTGTTAAGTCTTAATGGA TATAGTTATAATAGTTGAAAATGACTTGATGATTTATTATTTATTTCAGTTTATATTTTCATAATAAGCC GTTAGTGTACTAGAGACAGGCAGACATCTACCCTCTGTGTTACTGTATCTACTTCTTTATCAACAACCCA AGTTATAACTTTTGCCATATTCCATCTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 158557. Forward Primer - name:158557_F_cDNA_Mm.38166, sequence:ttcagaaaagggcaagactctc; Reverse Primer - name:158557_N_SP6_cDNA_Mm.38166, sequence:CCAGATGGAATATGGCAAAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18877 same embryo
	EMAGE:18882 same embryo
	EMAGE:18878 same embryo
	EMAGE:18881 same embryo
	EMAGE:18879 same embryo
	EurExpress:euxassay_015815 same experiment
	MGI:4822800 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source