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EMAGE:18898

2900092D14Rik RIKEN cDNA 2900092D14 gene ( MGI:1920350)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18898 EMAGE:18898 EMAGE:18898 EMAGE:18898 EMAGE:18898
"Pseudo-wholemount" of euxassay_015843. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015843_01 euxassay_015843_02 euxassay_015843_03 euxassay_015843_04
EMAGE:18898 EMAGE:18898 EMAGE:18898 EMAGE:18898 EMAGE:18898
euxassay_015843_05 euxassay_015843_06 euxassay_015843_07 euxassay_015843_08 euxassay_015843_09
EMAGE:18898 EMAGE:18898 EMAGE:18898 EMAGE:18898 EMAGE:18898
euxassay_015843_10 euxassay_015843_11 euxassay_015843_12 euxassay_015843_13 euxassay_015843_14
EMAGE:18898 EMAGE:18898 EMAGE:18898 EMAGE:18898 EMAGE:18898
euxassay_015843_15 euxassay_015843_16 euxassay_015843_17 euxassay_015843_18 euxassay_015843_19
EMAGE:18898 EMAGE:18898
euxassay_015843_20 euxassay_015843_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18898Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18898_wholemount_strong.wlz
18898_wholemount_moderate.wlz
18898_wholemount_weak.wlz
18898_wholemount_possible.wlz
18898_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18898_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
olfactory cortex ventricular layer
weak weak
homogeneousweak expression: see section 09 10 14 15
telencephalon ventricular layer
weak weak
homogeneousweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21
midbrain ventricular layer
weak weak
regionalweak expression: see section 06 07 08 09 11 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40535
Entity Detected:2900092D14Rik, RIKEN cDNA 2900092D14 gene ( MGI:1920350)
Sequence:sense strand is shown

>T40535
AGAAGATAGGCAAACCAAGCAGTTTTGCTTGTCATTGTGTGTTGTGTAAAGTAAGCTGACGCTTGCACTT
TTTGTTTTAAAGTCAATCCTTTGAATGTATTTACAAAATGAAATTGATGGTTGAAACACAGCAGAGAAGA
TATGAGAGTTTTTGTGCTAAGGCTCATATTGTTACACATGTAAATAATGAACATATTGTTGTATATCACC
AGGTCTTTAAAAAAAAAAGAATTTGGTCATTGTGTTCTTATGCAGCACAAATGTCTTGTGCTTCGTAAAC
TGCCCATTTGTTTCCTCCATTTCAGACTGCCTACTCTTCCCCTTCCAACCCAAAGGAAAGTGACAAAGTT
CTGGTCCATCAGCTTGGCTACTTTCAAACTAGGCCCAGTTATAGTTTACAGTAGAGTGTGGCTCCCGAGC
CACTATCCAAGCTTTGATCACCCCATCTACCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 162772. Forward Primer - name:162772_F_cDNA_mCG145686, sequence:AGAAGATAGGCAAACCAAGCAG; Reverse Primer - name:162772_N_SP6_cDNA_mCG145686, sequence:TGGGTAGATGGGGTGATCAAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18899 same embryo
 EMAGE:18897 same embryo
 EMAGE:18896 same embryo
 EurExpress:euxassay_015843 same experiment
 MGI:4822697 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS