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EMAGE:18929

LOC634321 (LOC634321)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18929 EMAGE:18929 EMAGE:18929 EMAGE:18929 EMAGE:18929
"Pseudo-wholemount" of euxassay_014218. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014218_01 euxassay_014218_02 euxassay_014218_03 euxassay_014218_04
EMAGE:18929 EMAGE:18929 EMAGE:18929 EMAGE:18929 EMAGE:18929
euxassay_014218_05 euxassay_014218_06 euxassay_014218_07 euxassay_014218_08 euxassay_014218_09
EMAGE:18929 EMAGE:18929 EMAGE:18929 EMAGE:18929 EMAGE:18929
euxassay_014218_10 euxassay_014218_11 euxassay_014218_12 euxassay_014218_13 euxassay_014218_14
EMAGE:18929 EMAGE:18929 EMAGE:18929 EMAGE:18929 EMAGE:18929
euxassay_014218_15 euxassay_014218_16 euxassay_014218_17 euxassay_014218_18 euxassay_014218_19
EMAGE:18929 EMAGE:18929 EMAGE:18929
euxassay_014218_20 euxassay_014218_21 euxassay_014218_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18929Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18929_wholemount_moderate.wlz
18929_wholemount_weak.wlz
18929_wholemount_possible.wlz
18929_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18929_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40438
Entity Detected:LOC634321, (LOC634321)
Sequence:sense strand is shown

>T40438
GTACAGGCGGGTATTTGAAGAGTACATGCAGGTTATTAGCCAGTGGTATCCAGACATCTGCATTGAAGGA
GAGAATTATCTCCCTCAACCAATTTATAGACACATAGCATCTTTCCTGTCAGTCTTCAAACTGACAGGAA
ATTATTAATAGGTTTAATAATTGATGGCAAAGATCCTTTTGCTTTTTTCGGCATGCCAGCTCCTAGCATC
TGGCAGTGGGGCCAAGAAAATAAGGTTTATGCATGTATGATGATTTTCTTCCTGAGCAACATGATTGAGA
ACCAGTGTATGTCAACAGGTGCATTTGGTATAACTTTAAATGATGTGCCAGTGTGGTCTAAGCTGGAATC
TGGATATCTTCATTCTATGCAACAACTTGTTCAAATTCTTGACGATGAAATGAAACTCAATGTGCATATG
GATTCAATCCCACATCTTCAATCACAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 163372. Forward Primer - name:163372_F_cDNA_mCG114479.1, sequence:GTACAGGCGGGTATTTGAAGAG; Reverse Primer - name:163372_N_SP6_cDNA_mCG114479.1, sequence:CTGTGATTGAAGATGTGGGATTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18931 same embryo
 EMAGE:18930 same embryo
 EMAGE:18932 same embryo
 EMAGE:18928 same embryo
 EurExpress:euxassay_014218 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS