EMAGE mouse gene expression spatial database

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EMAGE:18976

Ncan neurocan ( MGI:104694)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015922. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015922_01	euxassay_015922_02	euxassay_015922_03	euxassay_015922_04

euxassay_015922_05	euxassay_015922_06	euxassay_015922_07	euxassay_015922_08	euxassay_015922_09

euxassay_015922_10	euxassay_015922_11	euxassay_015922_12	euxassay_015922_13	euxassay_015922_14

euxassay_015922_15	euxassay_015922_16	euxassay_015922_17	euxassay_015922_18	euxassay_015922_19

euxassay_015922_20	euxassay_015922_21	euxassay_015922_22	euxassay_015922_23	euxassay_015922_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18976_wholemount_strong.wlz

18976_wholemount_moderate.wlz

18976_wholemount_weak.wlz

18976_wholemount_possible.wlz

18976_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18976_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

weak

regional

weak expression: see section 05 06 07 19 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 18 19

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 18 19

trigeminal v nerve

weak

regional

weak expression: see section 09

not examined

homogeneous

not examined expression: see section 17

ventral grey horn

strong

single cell

strong expression: see section 12 13 14 15

intermediate grey horn

strong

single cell

strong expression: see section 13 14

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 15 16 17

neural retina

weak

regional

weak expression: see section 01 02 03 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63368
Entity Detected:	Ncan, neurocan ( MGI:104694)
Sequence:	sense strand is shown >T63368 CTTAGCCCTGTGGGTAAAGATGCTGCATAAGCCTGAAGAAGTCCGTTTAAGCCCTGGGACCCATGTTTTA AAAGTGAAAAGCCAGGTAGGGTGTGAACCCTGGTGCCAGCATCCCTGTGCTGAGACTATAGGAAGCTCAT GAGCCAGCTAGCCTGGAGGATCACGGCAAACCCTTAAAGGGTAGCATTTGACCTCCACTGCACACTACAA TGTGGGTGATTCTCCAACACACGCACAACCCTCTCCCCCCACACACACTGCACAAGCACAAAATAAATAA GTAAAAATTTCAAAAACTGTAGGATTGGGTGGGTCAGGGCAGGGATGGGTGCACAGGTTCTGGGGTCCAT GCCCAGCTCTTCCTAAGTGTTTACTGAGCTGCATTGCTCACAGGAGGTAATGAAAGCCTGGGGCAGTTAC TGAATAGAAGCCGTGTAAAGCCTTGTGAGATGCCCTTGAATCCTCTCAGCAGCCCACGTGGTCCTTAGTC TCCCATTTCCACTTGATTGTAAGAGAAAGACAAAGTTACAGACTACGATAGAGGAGCTAGAGCAAGAATT TCCTTCTGTGCGTTATAATTACTGTAAGTGTTGATTTTTTGGACAGGGCCTCAGTCTGGCCTTGAATTCA CTCAGTAGCTGAGGATAACCTTAAAGTCCTGATCCTTCTGCCTCAGTTTCCCAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96444. Forward Primer - name:096444_F_cDNA_Cspg3, sequence:CTTAGCCCTGTGGGTAAAGATG; Reverse Primer - name:096444_N_SP6_cDNA_Cspg3, sequence:CTTGGGAAACTGAGGCAGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18970 same embryo
	EMAGE:18973 same embryo
	EMAGE:18974 same embryo
	EMAGE:18975 same embryo
	EMAGE:18972 same embryo
	EMAGE:18971 same embryo
	EMAGE:18969 same embryo
	EurExpress:euxassay_015922 same experiment
	MGI:4826610 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source