EMAGE mouse gene expression spatial database

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EMAGE:18982

Micall1 microtubule associated monoxygenase, calponin and LIM domain containing -like 1 ( MGI:105870)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015797. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015797_01	euxassay_015797_02	euxassay_015797_03	euxassay_015797_04

euxassay_015797_05	euxassay_015797_06	euxassay_015797_07	euxassay_015797_08	euxassay_015797_09

euxassay_015797_10	euxassay_015797_11	euxassay_015797_12	euxassay_015797_13	euxassay_015797_14

euxassay_015797_15	euxassay_015797_16	euxassay_015797_17	euxassay_015797_18	euxassay_015797_19

euxassay_015797_20	euxassay_015797_21	euxassay_015797_22	euxassay_015797_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18982_wholemount_strong.wlz

18982_wholemount_moderate.wlz

18982_wholemount_weak.wlz

18982_wholemount_possible.wlz

18982_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18982_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pharyngo-tympanic tube

weak

regional

weak expression: see section 01 02 03 04 22 23

midgut

weak

regional

weak expression: see section 07 08 09 10 11 12 13

bladder

moderate

regional

moderate expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40459
Entity Detected:	Micall1, microtubule associated monoxygenase, calponin and LIM domain containing -like 1 ( MGI:105870)
Sequence:	sense strand is shown >T40459 TGTCCTTGAAACTCATGTCACCTCACTCAGGAGCTGTATTTATGAGGGTTGCAAGTCCAGCCCCTCAGAG CGCTCTGTGCTGTGTCAGAAGGGAATGGGCTGAAGACAGTTCCGTTTCCTCTCCCTCATCTCCTTTGCTC TGTGGGAACCCAGGATTACCACACAGGGAGCTGCTGGCTGAGCTGGGGCACTGACTCAGGTCAGCCTGGA AGGGATGCTTTGGGTGGGTGTTCTCTCAGTCAGGCTTTCTGTCTTCCCCACGTCCGGGGATGATGGGCCC ATTCCTTTTTGTGGTCTGGGCAGTCTGTCACTGGGGACTGTGCCAGGCAGCTTATGGTCTAAGCACTGTT CTTACACTCGGGGACTTCAGAGTCCCTCCCTTTGTGCATCCTGGGAAGGAACTCACTGTATCTCAGTTGC CAGTGCCCCTTGGTAGTCTCTCCAGATTCACTTGCTGTCCTGGAACCAGGGCCACCACCTCAGAGGTCTT TCAGAGGAGCCCTATGACCCAGTTGCTGTAACCCAGGAGAGGGCTCTACCAGGCTGTCTCTGTGTCAAGG AAAGTTTGTATCTCAGTGAACAGGACAACCTTTCTCATAGTCACCTGTGTCCTGTCAACATGGGCCCATC AGAAGACATCCAGAATCCAGTTCTCCTACAGGCACAAGTTGTAAATGCTGACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 159317. Forward Primer - name:159317_F_cDNA_Mm.196480, sequence:TGTCCTTGAAACTCATGTCACC; Reverse Primer - name:159317_N_SP6_cDNA_Mm.196480, sequence:TGTCAGCATTTACAACTTGTGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18981 same embryo
	EMAGE:18980 same embryo
	EMAGE:18978 same embryo
	EMAGE:18979 same embryo
	EMAGE:18977 same embryo
	EurExpress:euxassay_015797 same experiment
	MGI:4826198 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source