Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19010

LOC622037 (LOC622037)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19010 EMAGE:19010 EMAGE:19010 EMAGE:19010 EMAGE:19010
"Pseudo-wholemount" of euxassay_015907. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015907_01 euxassay_015907_02 euxassay_015907_03 euxassay_015907_04
EMAGE:19010 EMAGE:19010 EMAGE:19010 EMAGE:19010 EMAGE:19010
euxassay_015907_05 euxassay_015907_06 euxassay_015907_07 euxassay_015907_08 euxassay_015907_09
EMAGE:19010 EMAGE:19010 EMAGE:19010 EMAGE:19010 EMAGE:19010
euxassay_015907_10 euxassay_015907_11 euxassay_015907_12 euxassay_015907_13 euxassay_015907_14
EMAGE:19010 EMAGE:19010 EMAGE:19010 EMAGE:19010 EMAGE:19010
euxassay_015907_15 euxassay_015907_16 euxassay_015907_17 euxassay_015907_18 euxassay_015907_19
EMAGE:19010 EMAGE:19010 EMAGE:19010 EMAGE:19010
euxassay_015907_20 euxassay_015907_21 euxassay_015907_22 euxassay_015907_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19010Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19010_wholemount_strong.wlz
19010_wholemount_moderate.wlz
19010_wholemount_weak.wlz
19010_wholemount_possible.wlz
19010_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19010_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40534
Entity Detected:LOC622037, (LOC622037)
Sequence:sense strand is shown

>T40534
AAGTGCTCATCTTCAGGCTTTCAAGGACTCAGGCTATGCAGGTAGGAAGACTGAGAAGGAGCCAAGTCCT
CCCATGGGACAGGAAGGGTTGGAAATTCATACAGGAGTGAACCTGCCCAAGGTTCATTCATAGCTAGCGG
GGAGCTGTGAGTGTGGGACTCACCCCACAGTCTGCTGATACCAAGGCAGATAGGTACTCAGTATCAAACT
ACAGCTTAGGTTTTCTATTACGTTTATTTGTTTTTCTTTGTGATTTTTTTTTTTTAATTAGTGGGCCGAA
TTGAATACACAAGTCTCCCACCCCCCTAACCCAGTACCGTCCCCACAGCAAGGAACACAGCAGGATGAGG
TTTCCTAACAGCCAGGAGCCCTCCAGTCACTGAGGCTGAACCAAAGGCAATTTCACTCTGCTGAAGTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 161697. Forward Primer - name:161697_F_cDNA_mCG145422, sequence:AAGTGCTCATCTTCAGGCTTTC; Reverse Primer - name:161697_N_SP6_cDNA_mCG145422, sequence:AACTTCAGCAGAGTGAAATTGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19013 same embryo
 EMAGE:19012 same embryo
 EMAGE:19008 same embryo
 EMAGE:19011 same embryo
 EMAGE:19009 same embryo
 EurExpress:euxassay_015907 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS