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EMAGE:19069

Hist1h1c histone cluster 1, H1c ( MGI:1931526)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19069 EMAGE:19069 EMAGE:19069 EMAGE:19069 EMAGE:19069
"Pseudo-wholemount" of euxassay_015954. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015954_01 euxassay_015954_02 euxassay_015954_03 euxassay_015954_04
EMAGE:19069 EMAGE:19069 EMAGE:19069 EMAGE:19069 EMAGE:19069
euxassay_015954_05 euxassay_015954_06 euxassay_015954_07 euxassay_015954_08 euxassay_015954_09
EMAGE:19069 EMAGE:19069 EMAGE:19069 EMAGE:19069 EMAGE:19069
euxassay_015954_10 euxassay_015954_11 euxassay_015954_12 euxassay_015954_13 euxassay_015954_14
EMAGE:19069 EMAGE:19069 EMAGE:19069 EMAGE:19069 EMAGE:19069
euxassay_015954_15 euxassay_015954_16 euxassay_015954_17 euxassay_015954_18 euxassay_015954_19
EMAGE:19069
euxassay_015954_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19069Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19069_wholemount_strong.wlz
19069_wholemount_moderate.wlz
19069_wholemount_weak.wlz
19069_wholemount_possible.wlz
19069_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19069_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 weak expression: see section 10 11 18 19 20
midbrain ventricular layer
weak weak
regionalweak expression: see section 09 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63463
Entity Detected:Hist1h1c, histone cluster 1, H1c ( MGI:1931526)
Sequence:sense strand is shown

>T63463
GTTGCAGCCAAGAAAAAGTAGGCTGCCTCCCCTAACAATCCAAAAGGCTCTTTTCAGAGCCACCACTCCC
ACTTAAGAGCTGTGACATTTGTGTGATTAATGAAGCAAGGTTGCAAGGGACTCGATCCCGGCAGCACTCG
CTCCCCCGGTGGGATGGGTGGGGTTGCGTGTCCCACCTAGACTTGTTTGTGCTGGGCTGCTCAGGAAGTG
CTATGGGCTGGAACACCCTCCCACGGCTGTCAGCAGCAGAAAGCAACCCTCGGCGCTAGTTCGTATTGGA
CTGCAAGGTGATTTGAAAGTTCCGCCCTGCTTGTGGGTTGGCTCACTCCGGCCCAGGACTCTAGTTCTTC
GTTTGATTGGGCGCCATCCCGAAGTTTGAGCTTCCTGCCTGGAGAAACGCATCCGGGGCTTGTAGGTGGC
TTCTATGCTCAGCCTCTTTTGTGGGGGTTAGCCTGGAAGTTTGGGAGAAGGTAGGGTGCTGGCGCCAACG
AACTGCCTTCCTTAATATTAAAGGCATGGGGTAGCTGCCGGAACGACTAGCTTGCACAGTTAAGTTGGAC
TCGGGACTGAGACGATTGCTTCTCGTACTTTCGGTTTGTTTGCCACAGCGATGGAGTTGTGGGTTGAACT
GCGTCACAACCTTTTGTCAGCCATTTTTTTTTTTCAAGTTTTTTACCATTACCAATTACACTTGCTTTGC
CTTATTTTGATCAGCATAGTTGTAGATTAACTTTGTTTTGCCTCAGCTTCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90666. Forward Primer - name:090666_F_cDNA_Hist1h1c, sequence:GTTGCAGCCAAGAAAAAGTAGG; Reverse Primer - name:090666_N_SP6_cDNA_Hist1h1c, sequence:GGAAGCTGAGGCAAAACAAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19073 same embryo
 EMAGE:19071 same embryo
 EMAGE:19070 same embryo
 EMAGE:19068 same embryo
 EMAGE:19072 same embryo
 EurExpress:euxassay_015954 same experiment
 MGI:4825375 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS