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EMAGE:19133

Mir343 microRNA 343 ( MGI:3718510)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19133 EMAGE:19133 EMAGE:19133 EMAGE:19133 EMAGE:19133
"Pseudo-wholemount" of euxassay_019423. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019423_01 euxassay_019423_02 euxassay_019423_03 euxassay_019423_04
EMAGE:19133 EMAGE:19133 EMAGE:19133 EMAGE:19133 EMAGE:19133
euxassay_019423_05 euxassay_019423_06 euxassay_019423_07 euxassay_019423_08 euxassay_019423_09
EMAGE:19133 EMAGE:19133 EMAGE:19133 EMAGE:19133 EMAGE:19133
euxassay_019423_10 euxassay_019423_11 euxassay_019423_12 euxassay_019423_13 euxassay_019423_14
EMAGE:19133 EMAGE:19133 EMAGE:19133 EMAGE:19133 EMAGE:19133
euxassay_019423_15 euxassay_019423_16 euxassay_019423_17 euxassay_019423_18 euxassay_019423_19
EMAGE:19133 EMAGE:19133 EMAGE:19133 EMAGE:19133 EMAGE:19133
euxassay_019423_20 euxassay_019423_21 euxassay_019423_22 euxassay_019423_23 euxassay_019423_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19133Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19133_wholemount_strong.wlz
19133_wholemount_moderate.wlz
19133_wholemount_weak.wlz
19133_wholemount_possible.wlz
19133_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19133_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70424
Entity Detected:Mir343, microRNA 343 ( MGI:3718510)
Sequence:sense strand is shown

>T70424
TCTCCCTTCATGTGCCCAGA
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-343 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19136 same embryo
 EMAGE:19137 same embryo
 EMAGE:19139 same embryo
 EMAGE:19140 same embryo
 EMAGE:19135 same embryo
 EMAGE:19138 same embryo
 EMAGE:19134 same embryo
 EurExpress:euxassay_019423 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS