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EMAGE:19209

Zfp874a zinc finger protein 874a ( MGI:3040703)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19209 EMAGE:19209 EMAGE:19209 EMAGE:19209 EMAGE:19209
"Pseudo-wholemount" of euxassay_009176. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009176_01 euxassay_009176_02 euxassay_009176_03 euxassay_009176_04
EMAGE:19209 EMAGE:19209 EMAGE:19209 EMAGE:19209 EMAGE:19209
euxassay_009176_05 euxassay_009176_06 euxassay_009176_07 euxassay_009176_08 euxassay_009176_09
EMAGE:19209 EMAGE:19209 EMAGE:19209 EMAGE:19209 EMAGE:19209
euxassay_009176_10 euxassay_009176_11 euxassay_009176_12 euxassay_009176_13 euxassay_009176_14
EMAGE:19209 EMAGE:19209 EMAGE:19209 EMAGE:19209 EMAGE:19209
euxassay_009176_15 euxassay_009176_16 euxassay_009176_17 euxassay_009176_18 euxassay_009176_19
EMAGE:19209
euxassay_009176_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19209Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19209_wholemount_strong.wlz
19209_wholemount_moderate.wlz
19209_wholemount_weak.wlz
19209_wholemount_possible.wlz
19209_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19209_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1191
Entity Detected:Zfp874a, zinc finger protein 874a ( MGI:3040703)
Sequence:sense strand is shown

>T1191
CCTCNAGNCTGTTGGCCTACTGGGTGCTCTCCCGTTGGATCTCTGACATGGAGGAAATGCTGTCCTTCTG
GGATGTGGCCATTGATTTTTCTCCAGAAGAGAAAGAATGCCTGGAGCCTGCTCAGTGGGATCTGTACAGG
GATGTGATGCTGGAGAATTTCAGCCACCTTGATTTCCTGGGTCTTGCTGTTGCTAAACCATACTTGGTAA
CATTTCTGGAGCAAAACCAAGGATCTTCGGGTGTGAAAAGCCAAACATCAGCCACCATTCCTCCAGGAAC
AACAGGCAATGAATTTAACAATCACAATGAGGCCATTGATCACAGCTCTCTAAGTATGCAATGCCAGAGA
ATTCATACAGGAGAGGAACCCTACTACTTTGAAGACTGTGGTAAGGCCCTTAGTTCTCAGGCAACACTTT
CTGTACTCCAGGGACATTTTATTGGCGACAAACCCTACAAGTGTAGAGAATGTCATAAAACTCTTAGTAG
TCGCTCATCACTTTTAATACACCAGAAATATCACACTGATGAGAAAACCTACAAGTGTGAAAAATGTGGC
AAAGGGTTTTTCCGT
Notes:The probe template was PCR amplified from IMAGE:2182181 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2182181 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19213 same embryo
 EMAGE:19212 same embryo
 EMAGE:19210 same embryo
 EMAGE:19211 same embryo
 EurExpress:euxassay_009176 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS