EMAGE mouse gene expression spatial database

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EMAGE:19221

Tmem120a transmembrane protein 120A ( MGI:2686991)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009162. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009162_01	euxassay_009162_02	euxassay_009162_03	euxassay_009162_04

euxassay_009162_05	euxassay_009162_06	euxassay_009162_07	euxassay_009162_08	euxassay_009162_09

euxassay_009162_10	euxassay_009162_11	euxassay_009162_12	euxassay_009162_13	euxassay_009162_14

euxassay_009162_15	euxassay_009162_16	euxassay_009162_17	euxassay_009162_18	euxassay_009162_19

euxassay_009162_20	euxassay_009162_21	euxassay_009162_22	euxassay_009162_23	euxassay_009162_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19221_wholemount_strong.wlz

19221_wholemount_moderate.wlz

19221_wholemount_weak.wlz

19221_wholemount_possible.wlz

19221_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19221_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 09 10 11 12 13 24 weak expression: see section 02 03 04 05 06 07 08 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 05

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 weak expression: see section 03 04 16 17 18 19

vagus x ganglion

moderate

regional

moderate expression: see section 07

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 weak expression: see section 14 15 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 09

cervical ganglion

moderate

regional

moderate expression: see section 08 weak expression: see section 16

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 weak expression: see section 09 10 11 12 13 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2399
Entity Detected:	Tmem120a, transmembrane protein 120A ( MGI:2686991)
Sequence:	sense strand is shown >T2399 TGGCCTCGAGCAGATTCGGACGAGGGAGGCTTCTCCGGCCGGCCGGGGTTGCAGCTCCGTCCTCCCCACT CGCGGCTCTCCGCGGCCAGTGGCGTGGACAAAGACATGCAGTCCCCGCCCCCGGACCCGCTGGGCGACTG CCTGCGCAACTGGGAGGATCTGCAGCAGGACTTCCAAGGTATCCAGAAGAAGCGGCTCCAGGAGCTGGCC TTGGTGCTGAAAAAATGCAGACCCTCTCTCCCATCCGAGTCCATGGAGGCTGCACAGGAGCTGGAAAACC AGATGAAAGAGCGCCAAGGCCTCTTCTTCGACATGGAAGCCTATCTGCCTAAGAAGAACGGGTTGTACCT GAGTCTGGTTCTGGGGAATGTCAACGTGACACTTCTGAGCAAGCAGGCTAAGTTCGCTTACAAAGACGAG TACGAGAAATTCAAGCTCTACCTCACCATCATCCTCATCGTCATCTCCTTCACCTGCCGCTTCCTGCTCA ACTCCAGGGTGACGGACGCAGCCTTCAACTTCCTGCTGGTCTGGTATTATTGCACGCTGACCATCC
Notes:	The probe template was PCR amplified from IMAGE:1196000 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1196000 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19219 same embryo
	EMAGE:19220 same embryo
	EMAGE:19218 same embryo
	EMAGE:19217 same embryo
	EMAGE:19216 same embryo
	EMAGE:19214 same embryo
	EMAGE:19215 same embryo
	EurExpress:euxassay_009162 same experiment
	MGI:4828766 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source