EMAGE mouse gene expression spatial database

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EMAGE:19363

Rprd1b regulation of nuclear pre-mRNA domain containing 1B ( MGI:1917720)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004846. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004846_01	euxassay_004846_02	euxassay_004846_03	euxassay_004846_04

euxassay_004846_05	euxassay_004846_06	euxassay_004846_07	euxassay_004846_08	euxassay_004846_09

euxassay_004846_10	euxassay_004846_11	euxassay_004846_12	euxassay_004846_13	euxassay_004846_14

euxassay_004846_15	euxassay_004846_16	euxassay_004846_17	euxassay_004846_18	euxassay_004846_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19363_wholemount_strong.wlz

19363_wholemount_moderate.wlz

19363_wholemount_weak.wlz

19363_wholemount_possible.wlz

19363_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19363_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6030
Entity Detected:	Rprd1b, regulation of nuclear pre-mRNA domain containing 1B ( MGI:1917720)
Sequence:	sense strand is shown >T6030 TCGGCGGAGAGCCCTGCAGCCTGTCAGCGGAGGCCTGGGCCGAGTGCGGTCGCTTTCCCCGTCTCATCGG GCGGCCCGGGCTGCTCCCCACCACCGCCACCATGTCCTCCTTCTCAGAATCGGCGCTGGAAAAGAAGCTC TCAGAGCTGAGCAACTCTCAGCAGAGCGTGCAGACCCTCTCCCTGTGGCTCATCCACCACCGCAAGCACG CAGGACCCATCGTCTCCGTGTGGCACCGCGAGCTCCGCAAAGCCAAATCAAATAGAAAGCTTACTTTTCT ATACTTAGCAAATGATGTCATCCAAAACAGTAAAAGGAAAGGACCTGAGTTCACAAGAGAATTTGAGTCT GTGCTTGTGGATGCTTTTTCTCATGTTGCCAGAGAGGCAGATGAAGGCTGTAAAAAACCTTTAGAAAGAT TGCTGAACATCTGGCAAGAACGAAGTGTGTACGGCGGCGAGTTCATACAGCAGCTGAAGCTGTCTATGGA GGACTCCAAGAGCCCTCCCCCCAAAGCAGAAGAGAAGAAGTCTCTAAAACGAACTTTTCAGCAGATACAA GAGGAGGAAGATGATGACTACCCTGGAAGCTACTCTCCCCAAGACCCTTCTGCAGGCCCTCTCTTGACTG AGGAGTTAATCAAAGCTTTGCAGGATCTGGAAAATGCTGCGTCAGGGGATGCTACTGTCCGACAGAAGAT CGCTTCCCTGCCTCAGGAAGTGCAAGACGTGTCGCTGCTAGAGAAAATTACAGACAAAGAGGCAGCTGAA CGTCTTTCAAAAACAGTAGATGAAGCATGTCTGTTACTAGCGGAATATAACGGGCGCCTGGCAGCGGAAC TGGAAGACCGGCGCCAGCTGGCTCGGATGCTGGTGGAATACACCCAGAACCAGAAAGAGGTTTTGTCAGA AAAAGAGAAAAAACTAGAAGAGTATAAACAGAAGCTTGCTCGAGTAACCCAGGTCCGCAAGGAACTCAAG TCCCACATTCAGAGCTTGCCAGACCTTTCGCTGTTGCCTAATGTCACAGGGGGCCTGGCACCTCTGCCCT CTGCTGGTGACCTCTTTTCAACTGACTAGGACAGGTATCATGCCCCAGGTTCCCATGTGTGCCAGAGAAA AGAAGCAAGTCACTGCTGGAGACAGTCCCCTGCCCCAGCTCCATCTCTTCAGCCAGCATCTGCCTCAGTA ATGAAGCGGGGAACTCTGACTTCTTACTCTCCTCCGGCTCCCTCTGAGCATGGTTCAGAGCCATGCAGAT GGACTCAGTTTTGATTCATATTTGCTAAGAACACAGACTCTCCCTCCCACTCATGTCTCTTGCCACTGCT GCTTTTCCATCTTTCGCTCTCTTACACCCATAAGCCATGAAAATCATGTGTACTTCTGGAAGCTTTCAAG AGAAGCTTGCCTCAGTGACAGCACATGCCATGGAAGTCTGAGCTGGCCTTGACTAGGTTCAGAACAGGCT CCCAATGAGGACTCAGGCTGCAGGGATTGGGCTTTCCTGGCCACCATTGCTCACAAGGTCGGCAGCATAG CTTTGCCACAAAGAGTTTGCCAAATTGCCGACATTTCCTGATCTAAACACTTCTGTTAGAGAAGTGTTGT CATGGTTAGGTGTGTTTATTTTTCGAGCATTCTAGAGAATCCAACAGTAAGATTCGAAGCTGATCTGAGA CATAAAAGTTATAGCGCAGGTACAGAGTGGCAGGGCTCACAAGTCGTTTGCCAGGCTTCTCACCACAGCC TTCCCTGCCACATTGCTCCAGGGTCCCCAGTTTCAGAGCACCTTCCTAAAGCAGGACCCTCTGTCCCCTG GGGCTGGGAAGGGAGCTGTTGGGATAGCATGCCCAGGCTGATAACTGCTCCACAGCTGGAGTCTGGGCTC TCGTGCATGTTGCTCTCACCCCGAAGCTTACAATAACGCCACCATGTGGACGCATCCACAGCATGGTGAC TGGACTTGAACATCTCTACAGATAGGACACTGAGCGTGCCAGGCAGTTCCTGCCACTATATGCATCCTTG CTCCCATGCAGAGAGAGGAGTACTCAAACAACTCACCTCCAGCAGAGGAAGGGAGTGGAGGGAGACTAAT TTGGGGTTGAATTTCATTATCATGCCAGCTGACATTATGACTGAATGATGTAGTTGCGATTTTTACGTCT AGTAAAGAGTGGGCCTGGCCCTGGAAATGACTGTAATCTGGCAGGCTTATATTTGACATTGGAAAGGGCA GAAACCATTTTGTCCTACTAGTGTGATAGGAATTGTCGCCCAGAGCTGAAGTCCAAAAGCCATAAGAAGG AATTCAGTGAAGGGCCTGAGAAGCTTCGTTGCTTTAAGTTGCTGTTTTCAGGATCACCAAAAAAAAAAAG CACACATCGTTTTTACACGTTAAAGCAAATCTGTGTGTCACCAAATAGGCAGGCTTTGGTTGAAGAAGCC GCCCCACCTGCTATGAGATGGGACTCAGTTCCACTCTGTCCAGCCTGGTCTTCGTGGAGGCTGTTATTCA CAGAGTGCAGGCTGCTGTTTGCTTGTTCACTCTGCCTTTTCTGTCAGCATCCTTTTCTCCAGAGGTTACA GGACAGCACTGACTCAGGTGAAACCCCTGTCTCTGCTGCGGAACACCTCCCTACACACCACGCCACTTTA CTGTCACATGCACACACCACTCTGAACACCCTCCAGACACTGCTTTCCCACAAAACAGACCAAAAGACTG TAGCAGCTCTGCTCTGATGACAACTTAGTGGGGTCAGCTCAATGGTGTTTATTCTTTGGAGTCTCTGACC AGCAATCAGTGCGTAACTCTTACAGCAGGATCAAGACGTGACCAGTAGCGATTATGTAAATGAATGTGTT GGCCTCATTACACCACTTCCAGCAGATTTCCTGTGAGGAAGTTAGTTTCGTTTTAATGAAATGCTTCAGT TTTTAATCTTCCTTGTTGTGTGCCTCCACCATGCCCCCAGTCTCCCAACTGTGTGCGAACTTCCCTTGTT TAACTTAAGTGAAACTTTTGTCCCTGAGAGTTGATCTGGCGGGTGGGGTGGGTGACTTCTGGTTTTGTGT TTCTTTAGGGCATCCGTAGGCCTCAAGGATCTTTCCTTTAGGGTCATATTCCTCAGAAAGTCTTCAGTCT TTCTTTGTTTTTGTTTTGTTTTTCTTAAAGGATATTTTTAAAGCTTAAATTTGTATATTAATTTAGGACT TTATTTAGAAGTATAGGCTGTCATTGGTGGCAGCAGTATATTCTGAAATGTCTCATAGATATATATTTTT GAATAAAGATGGTGTTGTTGACCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3488763 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488763 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19364 same embryo
	EMAGE:19359 same embryo
	EMAGE:19360 same embryo
	EMAGE:19361 same embryo
	EMAGE:19362 same embryo
	EurExpress:euxassay_004846 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source