EMAGE mouse gene expression spatial database

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EMAGE:19395

Icam1 intercellular adhesion molecule 1 ( MGI:96392)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006087. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006087_01	euxassay_006087_02	euxassay_006087_03	euxassay_006087_04

euxassay_006087_05	euxassay_006087_06	euxassay_006087_07	euxassay_006087_08	euxassay_006087_09

euxassay_006087_10	euxassay_006087_11	euxassay_006087_12	euxassay_006087_13	euxassay_006087_14

euxassay_006087_15	euxassay_006087_16	euxassay_006087_17	euxassay_006087_18	euxassay_006087_19

euxassay_006087_20	euxassay_006087_21	euxassay_006087_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19395_wholemount_strong.wlz

19395_wholemount_moderate.wlz

19395_wholemount_weak.wlz

19395_wholemount_possible.wlz

19395_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19395_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 07 08 09 10 11

left lung

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10

right lung

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6491
Entity Detected:	Icam1, intercellular adhesion molecule 1 ( MGI:96392)
Sequence:	sense strand is shown >T6491 CTGCTACCTGCACTTTGCCCTGGCCCTGCAATGGCTTCAACCCGTGCCAAGCCCACGCTACCTCTGCTCC TGGCCCTGGTCACCGTTGTGATCCCTGGGCCTGGTGATGCTCAGGTATCCATCCATCCCAGAGAAGCCTT CCTGCCCCAGGGTGGGTCCGTGCAGGTGAACTGTTCTTCCTCATGCAAGGAGGACCTCAGCCTGGGCTTG GAGACTCAGTGGCTGAAAGATGAGCTCGAGAGTGGACCCAACTGGAAGCTGTTTGAGCTGAGCGAGATCG GGGAGGACAGCAGTCCTCTGTGCTTTGAGAACTGTGGCACCGTGCAGTCGTCCGCTTCCGCTACCATCAC CGCGTATTCGTTTCCGGAGCGTGTGGAGCTGAGACCTCTGCCAGCCTGGCAGCAAGTAGGCAAGGACCTC ACCCTGCGCTGCCACGTGGATGGTGGAGCACCGCGGACCCAGCTCTCAGCAGTGCTGCTCCGTGGGGAGG AGATACTGAGCCGCCAGCCAGTGGGTGGGCACCCCAAGGACCCCAAGGAGATCACATTCACGGTGCTGGC TAGCAGAGGGGACCACGGAGCCAATTTCTCATGCCGCACAGAACTGGATCTCAGGCCGCAAGGGCTGGCA TTGTTCTCTAATGTCTCCGAGGCCAGGCGCCTCCGGACTTTCGATCTTCCAGCTACCATCCCAAAGCTCG ACACCCCTGACCTCCTGGAGGTGGGCACCCAGCAGAAGTTGTTTTGCTCCCTGGAAGGCCTGTTTCCTGC CTCTGAAGCTCGGATATACCTGGAGCTGGGAGGCCAGATGCCGACCCAGGAGAGCACAAACAGCAGCGAC TCTGTGTCAGCCACTGCCTTGGTAGAGGTGACTGAGGAGTTCGACAGAACCCTGCCGCTGCGCTGCGTTT TGGAGCTAGCGGACCAGATCCTGGAGACGCAGAGGACCTTAACAGTCTACAACTTTTCAGCTCCGGTCCT GACCCTGAGCCAGCTGGAGGTCTCGGAAGGGAGCCAAGTAACTGTGAAGTGTGAAGCCCACAGTGGGTCG AAGGTGGTTCTTCTGAGCGGCGTCGAGCCTGGGCCACCCACCCCGCAGGTCCAATTCACACTGAATGCCA GCTCGGAGGATCACAAACGGCGCTTCTTTTGCTCTGCCGCTCTGGAGGTGGCGGGAAAGTTCCTGTCTAA AAACCAGACCCTGGAACTGCACGTGCTGTATGGTCCTCGGCTGGACGAGACCGACTGCTTGGGGAACTGG ACCTGGCAAGAGGGGTCTCAGCAGACTCTGAAATGCCAGGCCTGGGGGAACCCATCTCCTAAGATGACCT GCAGACGGAAGGCAGATGGTGCCCTGCTGCCCATCGGGGTGGTGAAGTCTGTCAAACAGGAGATGAATGG TACATACGTGTGCCATGCCTTTAGCTCCCATGGGAATGTCACCAGGAATGTGTACCTGACAGTACTGTAC CACTCTCAAAATAACTGGACTATAATCATTCTGGTGCCAGTACTGCTGGTCATTGTGGGCCTCGTGATGG CAGCCTCTTATGTTTATAACCGCCAGAGAAAGATCAGGATATACAAGTTACAGAAGGCTCAGGAGGAGGC CATAAAACTCAAGGGACAAGCCCCACCTCCCTGAGCCTGCTGGATGAGACTCCTGCCTGGACCCCCTGCA GGGCAACAGCTGCTGCTGCTTTTGAACAGAATGGTAGACAGCATTTACCCTCAGCCACTTCCTCTGGCTG TCACAGAACAGGATGGTGGCCTGGGGGATGCACACTTGTAGCCTCAGAGCTAAGAGGACTCGGTGGATGG AGCAAGACTGTGAACACGTGTGACCCGGACCCACCTACAGCCCGGTGGACCTTCAGCCAAGAAACGCTGA CTTCATTCTCTATTGCCCCTGCTGAGGGGCTCCTGCCTAAGGAAGACATGATATCCAGTAGACACAAGCA AGAAGACCACACTTCCCCCCGACACAGGAAAGCTGAGACATTGTCCCCAACTCTTCTTGATGTATTTATT AATTTAGAGTTTTACCAGCTATTTATTGAGTACCCTGTATATAGTAGATCAGTGAGGAGGTGAATGTATA AGTTATGGCCTGGACCCTGCTGCAGATGCTGTGAGAGTCTGGGGGAAGATCACATGGGTCGAGGGTTTCT CTACTGGTCAGGATGCTTTTCTCATAAGGGTCGACTTTTTTCACCAGTCACATAAACACTATGTGGACTG GCAGTGGTTCTCTGCTCCTCCACATCCTGGAGCGTCCCAGCACCTCCCCACCTACTTTTGTTCCCAATGT CAGCCACCATGCCTTAGCAGCTGAACAATCGAGCCTCATGCTCATGAAATCATGGTCCCAGGCGGCTCCA CCTCAAAGAGAAAGCCTGGAAGGAAATGTTCCAACTCCTTAGAAGGGTCGTGCAAGCTGCTGTGGGAGGG TAAGCAACCCTCCCAGCACAGAAACCTTTCCTTTGAATCAATAAAGTTTTATGTCGGCCTGAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3588949 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3588949 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19397 same embryo
	EMAGE:19400 same embryo
	EMAGE:19396 same embryo
	EMAGE:19398 same embryo
	EMAGE:19399 same embryo
	EurExpress:euxassay_006087 same experiment
	MGI:4825498 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source