EMAGE mouse gene expression spatial database

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EMAGE:19430

Tmem175 transmembrane protein 175 ( MGI:1919642)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004820. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004820_01	euxassay_004820_02	euxassay_004820_03	euxassay_004820_04

euxassay_004820_05	euxassay_004820_06	euxassay_004820_07	euxassay_004820_08	euxassay_004820_09

euxassay_004820_10	euxassay_004820_11	euxassay_004820_12	euxassay_004820_13	euxassay_004820_14

euxassay_004820_15	euxassay_004820_16	euxassay_004820_17	euxassay_004820_18	euxassay_004820_19

euxassay_004820_20	euxassay_004820_21	euxassay_004820_22	euxassay_004820_23	euxassay_004820_24

euxassay_004820_25	euxassay_004820_26	euxassay_004820_27

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19430_wholemount_strong.wlz

19430_wholemount_moderate.wlz

19430_wholemount_weak.wlz

19430_wholemount_possible.wlz

19430_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19430_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6518
Entity Detected:	Tmem175, transmembrane protein 175 ( MGI:1919642)
Sequence:	sense strand is shown >T6518 CGGACCGCCCCAGAGCCCGCCGCTCGCGCCTGACGCCTCAAGGGTTGAGACATCCCTCTGATATGCTCAG AGCTACACTTCTTCCCCGGCTGCTGTGACAACTTGTTGGTCCCACCATGTCCAGGCTCCAGACTGAGGAG CAGGCAGTGGATTCCGAGGGAGACTCATCCCTACACAGGAGAAATGAGGAGGGGACACAGAGCTCCCACC GCATGCTGGGCTTCAGTGATGCACTGCTATCCATCATCGCCACTGTCATGATCCTGCCTGTGACCCACAC AGAGATCTCACCAGAACAGCAGTTTGACAAAAGTATACAGAAACTCCTAGCAACTAGGATCGCTGTCTAC CTCATGACATTTCTAATCGTAACTGTGGCCTGGACAGCACATACCAGATTGTTCCAGGTTGTTGGAAAAA TAGATGATACACTTGCCTTGCTCAACCTGGCCTGTATGATGACTATCACCCTCCTGCCCTACACATTTTC ACTAATGGTAACATTCCCTGATGTGCCCCTGGGCATCTTCCTGTTCTGCGTGTGTGTGATCGCCATTGGA TCTGTGCAGGCAATGATTGTGGGCTATGCCTTCCACTTCCCACATCTGCTGAATCCACAAATCCAGTGCT CTACACGTAGGGATCTATCCCGGCGGCATATCCTGCACCTGGTCCTCCGTGGCCCAGCACTGTGTTTTGT TGCGGCTGTCTTTTCCCTCTTCTTCTTCCCCTTGTCATACCTGCTGATGGTGACTGTCATCTTCCTCCCT CACATCAGCAAGGCTACCACCTGGTGCAAAGACAAGTTGATGGGCCAAAGGGAGTCTCCAGCTCACGACA TGGAGCCCTTCAGTATTGACCTGCATGCGCCCCTCAGCAAAGAGCGGGTGGAAGCTTTCAGCGACGGTGT CTATGCCATCGTGGCCACACTCCTCATCCTGGACATCTGTGAGGACAATGTTCCGGATCCCAAGGATGTG CAAGAGAAATTCAGTGGCAGCCTTGTGGCTGCCCTGGGTGCATATGGGCCACAATTCTTGGCATACTTCG GCTCCTTCGCCACAGTGGGTCTCCTCTGGTTTGCTCACCATTCACTCTTCCTGCATGTCCGGAAGGCTAC ACAGACCATGGGGTTACTCAACATACTATCGCTGGCCTTTGTGGGTGGCCTTCCTCTGGCCTACCAGCAG ACCTCAGCTTTTGCCCGGCAACCCCATGATGAACTTGAGCGTGTGCGTGTCAGCTGTGCCATCATCTTCT TTGCCAGCATCTTCCAGTTTGCCATCTGGACTACAGCCCTGCTGCATCAGACAGAAACACTGCAGCCAGC TGTGCAATTTGGTGGGCAGGAGCATGCTTTCATGTTTGCCAAGCTTGCACTGTATCCCTGTGCCAGCCTG CTGGCCTTTGCTGCCACCTGCCTACTGAGTCGTTTCAGCACAGCCATCTTCCACCTCATGCAGATTTCAG TGCCCTTTGCATTCCTGTTGCTCCGTCTCCTTGTACGCCTTGCCCTGGCAGGCCTTCAAGTCCTACGGGG CCTCTGGCCACACCATCCCCAACAAGACCAGAGTGAACCTGAGGCACAGTCCCAGCTCTTGCCTGACCCC TGCTAATGCCAAGAAAGCCACTCCTGCCTATCCTAGGCATATTGTAAGGGGTCCTATGGAGATGTCTTAG TACAAGTTTATTGTGACACTGGGGCTGTGGATATATTGGCCTGGGTATTGTTTACATTGTGAAATATCAA GATCTATTTCAAGAGTATGAACATTGTTCACACTTTGCAGTGAAGGGTTTCATCGGAACTGAATTTATTG TTTTTACCCATTTACTTTCCTCCCTTGCCCACAACATTGCCAGGGGAATTTATCTGTCTGATTAACCTGA ACAAGCCACTTTGAGCTTGAGATCTTGTTTGAGGCAGCAGCATCCAGCCAGGATCAAGGGTCTATCCTAT GCCTTGCTCTGACCTGCATGTGGCCATACAGGCGATCCTCCTTCTTGGAACGTTCTAGCAATGGAGTCTA GGCTAACTCAGCCTGAGAAGCTCCTGTCTGGCTCACATCCTCAAAAGTACAGAGCTTCCCTGGCGGGACT GCCAGTCTGTTTGCCCATTCCTGTACCAGTTGTTAGACAAAGACAAGAGTCAGCTATGGGACAGCAAAAC CCACACTGTGTATTTGGCCCAGATATAACTTAGATCACATTCAGGTAGTGATAAATTACACACAAAGACA GGACCAGTCAGTGCACAGGACACACTCCACATAAAGACAGTCTCACTTAAATACAGAGGATATGCTACAC TGACCGTAATTTACAAACAATTCCTACAGATTGAAATAAAAATGAAAAATTACATCACAAAGAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3590497 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3590497 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19425 same embryo
	EMAGE:19428 same embryo
	EMAGE:19429 same embryo
	EMAGE:19426 same embryo
	EMAGE:19427 same embryo
	EurExpress:euxassay_004820 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source