EMAGE mouse gene expression spatial database

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EMAGE:19441

Trak2 trafficking protein, kinesin binding 2 ( MGI:1918077)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005138. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005138_01	euxassay_005138_02	euxassay_005138_03	euxassay_005138_04

euxassay_005138_05	euxassay_005138_06	euxassay_005138_07	euxassay_005138_08	euxassay_005138_09

euxassay_005138_10	euxassay_005138_11	euxassay_005138_12	euxassay_005138_13	euxassay_005138_14

euxassay_005138_15	euxassay_005138_16	euxassay_005138_17	euxassay_005138_18	euxassay_005138_19

euxassay_005138_20	euxassay_005138_21	euxassay_005138_22	euxassay_005138_23	euxassay_005138_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19441_wholemount_strong.wlz

19441_wholemount_moderate.wlz

19441_wholemount_weak.wlz

19441_wholemount_possible.wlz

19441_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19441_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 moderate expression: see section 20 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8819
Entity Detected:	Trak2, trafficking protein, kinesin binding 2 ( MGI:1918077)
Sequence:	sense strand is shown >T8819 CTTACAGCTGCAGATGTCACCTGACTCATCGCAGAAAATCTGTTCTAAAGAAAATATCCATATGACCACG CCCATTTCTTTACTAAATGGCTTAATGGATTTCCTTTCCTGCGATTCATACGAAAGCTGCCCCTTTCAAC CAAAGCAGGAAAGGATTCTGCATGAGTCAATCCCAGAATGCCATTTTCAAATCACGAACAGGTGAAGCAA ACCTCATGAGTAGCAATCATAGAGACTCGGAGAGCATCACTGATGTCTGCTCCAACGAGGATCTCCCTGA AGTCGAGCTGGTGAACTTGCTAGAAGAACAGCTGCCACAGTATAAGCTAAGAGTAGATTCTCTTTTTCTC TATGAAAACCAAGACTGGGCACAGTCATCACACCAGCAGCAGGATGCGCCTGAGACCCTCTCCCCAGTCC TTGCTGAAGAGACGTTCCGCTACATGATTCTAGGCACAGACAGAGTGGAGCAGATGACCAAAACCTACAA TGACATTGACATGGTCACACATCTCCTGGCAGAGAGGGATCGAGATCTAGAGCTAGCTGCCCGAATTGGG CAAGCTCTTTTAAAGCGAAACCATGTCTTATCTGAGCAGAATGAAGCTCTGGAGGAGCAGTTGGGACAAG CCTTTGATCAAGTTAATCAGCTGCAGCACGAGCTCTCCAAGAAAGACGAGCTGCTCCGAATCGTCTCTAT TGCCTCCGAGGAGAGTGAAACGGATTCTAGCTGCTCCACGCCCCTTCGGTTCAATGAGTCCTTCAGCTTA TCTCAGGGGCTGCTGCAGCTGGACATGCTGCATGAGAAGCTCAGGGAGCTGGAAGAAGAGAACATGGCCC TACGATCCAAGGCTTGTCATATCAAGACAGAAACATTTACCTATGAAGAGAAGGAACAACAGCTCGTCAA TGACTGTGTTAAAGAACTTCGTGAGACCAATGCACAGATGTCCAGAATGACTGAAGAGCTGTCGGGAAAG AGCGACGAGCTGCTCCGGTACCAAGAGGAGATCTCCTCCCTGCTGTCTCAGATCGTAGACCTTCAGCACA AACTGAAAGAGCATGTGATCGAGAAGGAAGAACTAAGACTTCACCTGCAGGCTTCCAAAGATGCCCAGCG ACAGCTGACAATGGAGCTTCATGAGTTACAGGACAGGAACATGGAATGCCTGGGAATGTTACATGAGTCC CAAGAAGAAATAAAAGAGCTTCGTAGCAAGTCCGGCCCTTCTGCTCATCTCTGCTTCTCCCAGTCTTACG GCGTTTTTACAGGGGAATCGTTGGCAGCTGAGATTGAAGGGACCATGCGTAAAAAGCTGAGTTTGGATGA GGAATCTGTGTCTAAACAAAAGGCCCAGCAGAAACGGGTGTTTGATACTGTCAAGGTTGCCAATGACACT CGGGGCCGCTCTGTCACCTTCCCAGTCCTGCTACCCATCCCAGGCTCCAACCGTTCAAGTGTCATCATGA CAGCAAAGCCCTTTGAGTCTGGTGTCCAGCCAGCAGAGGACAAAACACTCCTGAGCCCAGGGGGCAGCAC AGAGGTTCCAGGAAACTCGCAGCCCACAAACCCCCCAGGATCCCCTGAAGATAGTGATCTGGCTACTGCA TTGCATCGCCTCAGCCTGAGAAGACAGAACTACCTAAGTGAGAAGCAGTTCTTTGCTGAGGAATGGGAAC GGAAGATCCAGATCTTGGCTGAGCAGGAGGAAGAAGTCAGCAGCTGTGACGCCCCCACGGAGAACCTTGC CTCAGTCTGCACTGACCAGTCGGAGACCACGGACCTCGGCAGTGCCAGCTGCCTTCGAGGCTTCATGCCA GAGAAGTTACAGATTGTCAAGCCCCTAGAAGGATCACAGACTCTGCATCACTGGCAGCAGCTTGCTCAGC CGAACTTGGGAACCATTCTTGATCCACGCCCAGGCGTCATCACTAAAGGCTTCACACAGGTGCCCAAGGA TGTTGTCTACCACATCTCGGACTTAGAGGAGGATGAAGAAGAGGGCATCACTTTTCAGGTTCAGCAGCCT CTTCAACTGGAGCAGAAACCTGCACTGCCCACGCCAGTAACGGGAATCTTTCTGCCGCCCATGACCTCAG CGGGTGGACCAGGTACAGTTGCAACTTCGAACCCAGGAAAGTGTCTCTCATTCACCAACTCGACATTCAC CTTCACCACCTGCAGGATCTTACATCCCTCTGACATCACTCAGGTCACCCCTAGCTCCGGTTTCCCATCA CTGGCCTGTGGCAGTAGTGCGAGCAGCTCATCCAACACGGCTGTGAACTCCCCTGCTGCATCCTATAGAC TAAGCATTGGCGAATCCATCACAAACCGCAGGGACTCCACCATAACCTTCAGTAGCACCAGGAGCTTAGC CAAACTCCTGCAGGAGCGAGGCATCTCTGCTAAAGTGTACCACAGCCCGGCTTCAGAAAACCCCCTCCTG CAGCCTTGCCCCAAGGCCCTGGCCACCCCCTCCACACCACCAAATTCCCCAGCGCAGTCACCGTGCTCCT CCCCCTTGCCCTTTGAACCCCGAGTGCATGTCTCCGAGAATTTCTTGGCTTCCCGACCAGCTGAAACATT CCTGCAAGAAATGTATGGCTTGAGACCTTCAAGGGCCCCTCCTGATGTTGGACAGCTGAAGATGAACTTG GTAGACAGGCTGAAGAGGCTGGGAATAGCCAGGGTGGTCAAGACCCCTGATCCCCAGGAGAACGGAAAAA GCCGAGAAGCAGAAATGGGTCTTCAAAAACCAGACCCTGCTGTTTATTTAAATTCAGGTGGCAGTTTATT GTGTGGACTGAGGAGGAATCAGAGTCTTCCGGTCATGATGGGTAGCTTTGGAGCCCCAGTTTGCACAACC TCACCCAAAATGGGTATCCTGAAGGAAGATTGAGGCTCACCACTAACTGACCTCGTGTATAAATTAGCAC ATGAAGGAGAGAGATGCACTGAGAGTGGAGACCGGCCTAACTTGAGAGAAAAGGGATGTTGCAGAAGGAC TGTGAATGTGGAGAGAGCGCAGTGAAGGGATAGGACGTTTGTTTGCAAGAGGGTCTTTGAGGATGGAGTC TAGTAAACAAAGTGGGGACAAGTGATGACCAGTTTGGAGACAAATGGAAGAAAGGGTTAGTTTGCTCCTT CAGCTACGTTTCCTTGCCTTGGAAATAGTGAATAGAAAACTAAACCAAATTCTATTGAAATGTGACAGTA ATGCTAAACTGCTAGCACCTTTGTCCCTAGAGAACACAAGCCGGAAGCGCCTGCCTAGGAAGGAAACCTG GAACAGGCGATCCTGGAGAGTTGGCGCCTGTGAAGTTGTCTGTTACTGAGCCAGTGGGTGATACCGTCCC TGCTGGTCCCTGGTCAAATGCATCCATCCAATGGTGCACTCTCAGCATTAGCAAAATAAGAAAATTTATC TTGTCCTTTACATCAAAAATGAGACTTTCTTATTTGGGGACTTTGAGGCACTGAAAACCCAAACACTGCC CAGATAGATTCCTTGGTATTTATGATTACTAAATCTTCAGTTTTTTTAAGCTTGTATTTTTGTACAACAC AACAAAAAAATGTAAAATAACAATATGAGTGGACTTGGAGGGATTTAAGAGGGAAAAAGGGGTAAATTCG AATCAAAAACATTTTTAGGATATTTCTTCTGAACAATACGGTGGCCCAGGAATGCAAACACGATTGTGTG TTTCTCCGTTTTCCTTGGATGCCAGAAGGGTTTAAACCAGAAGTGCAATCAGTCAGTAGGCAGTAGATTA TGTTGTATATTTATATATTTAAATTTTTAAAGAAAAGTTAGTTGTAACTAACTTTTTCCAAAGTGTGCTA TGCATATTTTTAATGAAAGATGACATGTATTTGCACAAAAATTCTCAGGCACATTAAATTATAAACTGAA GTAAAACCCAGGTGCTTGCTTTGAAAGTGTGTTTTTGTTAGATTTTATTGTTTTCCTTGTGTACATTAAA GCACCTGCCTGTCTCTTGGTATCTGCAGAAGTAGAGAAGGGTGTTTGTTTATGGGAGAGCCAAGACTTCT TTTCCTCTGATTTCTTTAACAAGCTGAAAAGCAAGTGAGGCCTGAGGCTGGGGTTTAGGCCTTCAGCCTG TGGTGCAGGCCTCACGCTGTTGGGGGGGGAGGGGGGCAGATCTGTGCTCTGAGGTGGCAGGCCATGGTCT GCCTCTGCAGCTGACATGGCTTTGTCTCCGTGGGGTTGTTCAAGCACCTGGGGAGGAGATAAGGGGCAAC TGTTGGTTTTGTTTGTCAAGGCAGAAGGTCCTTTACTTGGTCCTTTGGTGCTTAACTTGCAAATATTTTG CTTCAGAGAAATTCTTTTCTGCCAGGGAGTTTTGCCTTTCTCTCTTGAGCCTCCTCCCCAAGAAGAGATG CTCTCAGAGGGTTAGAAAAGCCAGCCTCCACACAGTGGTAAGAGCATATGAGTTTTCTATATTTCTAGAA TCTTTAATACAACTTTTCTAAATTTTTCAGAAGCAAAGGGTGGAGAGAGATGTCCCTCCCTTCGCCACCT TCAGCCCCTTCGTCCAGACTAGCTGCTGTCAACAGTTCATCGCACACATGCACACATTTATGAACGACTC TGGGTTAGTGAGTGACCCTGCCTGAAGCAGGCAAGCGGGCATTAGCAAGAATTCTACTGGTTAAATTTCC TAAATATTCCAATTCCTGTTTTAAAAACAGCATTTGCGTTTCTAGTCTATAAATAACTGAGTACCACCTC CCTCAGCAGGACCTTAAAGCTTTGCCTTGGTTCCTGAAAAAAGGACTCTTCCTGCTACTTGGACAGTTAC AGCTTCTAAGGAAGGAACAGCACAGATAGGGAAGCCTGGGGCTAAGGGGCCAGTAAAATGTTAAATAACC AGGTTTACACAAAGGACAGAAACCACACTACTGAGTTAGAGATTGTGGAGTTCTGTGGTGACAATAATTC TGTCCTTCTGTCAGTGCCTAAGTGGCCACTGGAAGCAGAGTTTTGCCTTGGAAGCCAGATGAATATGGCC AATTAAAGAAAGCCAAACTTACGTTTTTGAAAATTAAATTGATCCTGATGTTCTGTCAGAACAAATTGTA CCTGTGCATGTTTGTCGCTGAAATGAATCCTGTCTAAAGCTGGGCCTTGCTGACTTTCAGTTTCCACCTA AGATGATTTGGGTACAGCCTGAGAATCTGTTCCATGGGACTATCTGCAAAATATAACAAGAACTGGAACT TTCCAGTAAGTTCTTCCTCCACAGGACTCTTAACTGTTCTCGTTTTGTTTTTGTTATCATTCAGAGTCAG CTGGTATGTACTTTCATGCTTGCCTGGGAAAACTCAACTGTTTTTTCCTTCAAGATTTTCTGTGATGATT TCTCAGGTACTTTCAAGTAGCCTGTTACCTATTCAGAGAGTCTCCTTGAGCTCAGAATTAAAATTGCCCT GAATTTTTGAAGATCCAAAATCTTATGTAAATAGCCATTGGTAGTTAAGTGGGTTTTATTTTTTAGTCTT GTGCTTGCCCTTGGTCTGACATAAGTGATCAGACTGCCAGGAGAAGATGTTAGAGAAGCTCACTAGAGCC TACCATCTGTTGAAGGCACATTTTACACAGTATTCCAAGCCCCTAGTGCATAATTAGTAGTGGGTGGTTC TCACATCAGCTTGACATCTGGAGTTAGGGTTGTCCGTCTGGGGCCTTGTCTGCTGTCAGGTGTGTACAGA CCTGTGCTTGTAGTGTACTGCAGGCATGCACACTAGTCCACTGCCAAGTGTGGCCCTAGGCATGGGATCC CGGGGGCTCCAGGAAAATGCTATTGTGTCATATCTGGAACACAACTTTGTCAAGTCACTCTTTATGCACC TTCCTTTGCTTTATCAATTGTAAATCAGATAATAAAATGTAAATCAGTTAATAAACATGAGGCTTCTTAA ATTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6410345 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410345 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19444 same embryo
	EMAGE:19442 same embryo
	EMAGE:19440 same embryo
	EMAGE:19443 same embryo
	EMAGE:19445 same embryo
	EurExpress:euxassay_005138 same experiment
	MGI:4828891 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source