EMAGE mouse gene expression spatial database

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EMAGE:19448

Aph1c anterior pharynx defective 1c homolog (C. elegans) ( MGI:1915568)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005139_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19448_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19448_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8824
Entity Detected:	Aph1c, anterior pharynx defective 1c homolog (C. elegans) ( MGI:1915568)
Sequence:	sense strand is shown >T8824 CGAGCCCGGAGTACCTGGCAGGCCTGCCCCGCCCCGCATTCCGCTAAGAAATCGTCCCAGTCACCTCGGA TCCACTCAGGTCCCCCGGGACTGGCTTCTGTGGGTACCATGACGCTGCCTGTGTTCTTCGGTTGCGCCTT CATCGCCTTCGGGCCCGCGTTCGCACTCTACCTCTTCACCATCGCCACCGACCCTTTGCGCGTCATTTTC CTCATCGCTGGTGCTTTCTTCTGGTTGGTGTCTCTCCTGCTTTCGTCCATGTTTTGGTTCCTAGTGAGAG TCATTACTAACAACAGAGATGAATCAGTACAGAATTATCTGCTCATCTTCGGAGCGTTGCTCTCTGTCTG TATCCAAGAGCTGTTCAGGCTCGCATATTATAAGCTGTTAAAAAAAGCCAGTGAGGGTTTGAAAAGCATA AACCCGGAGGAGGACATAGCACCCTCGATGCGACTGTTGGCGTATGTTTCTGGCTTGGGCTTTGGAATCA TGAGTGGAGTGTTTTCCTTTGTGAACACCCTGTCTAACTCCTTGGGGCCAGGCACAGTGGGCATTCATGG TGATTCTCCTCAGTTCTTCCTTAATTCAGCTTTCATGACACTGGTCGTCATAATGCTGCACGTGTTCTGG GGCGTTGTGTTCTTTGATGGCTGTGAGAAGAATAAGTGGTACACCCTCCTCACGGTTCTCCTGACGCATC TGGTGGTGTCCACCCAGACTTTTCTAAGTCCTTATTATGAAGTGAATCTGGTAACAGCATATATAATCAT GGTGCTCATGGGCATCTGGGCATTTTATGTTGCTGGAGGCAGCTGCCGAAGCCTGAAATTCTGCCTGCTC TGCCAAGACAAGGATTTTCTTCTTTACAACCAACGCTCCAGATAACCTCTGACCATCAGCACCTCTAAGC AGCAGCCTGTGTCTTTAGAGGAAGCACAACTGGGCCCTGTTCTAAAAAGACCCCTGTCTCTTGGAACTTG GAAGCCACAGAACTGTCCAGCCTGGAACTGTCCTGTGCTTTCATGCAGCAGCCTCTGGAAGAGCTGCAGT GTAGCCTGCAGCCTGGCTGCACATTTGAAGCATCTGGGGAAACAAGGACTGATGCTTCTGGGCTTCTCCA GACTTGATTATCCTGGATGTGCAGGACAGCTGAGAGTCACTGGGGTCCAATGGGGAGTTCGAGAAAACAC CCAAAAGCTGTTTAGTGTTGGTCTTCTAAGCCAACTTCAGCTGAGCAAGTAAATATCTGAATGTGGTAAC CAGCGTAGCCCTTGTCTTTTTATGTACTGATGTCACAGACTCCAGGGCTGTTCTGTCTGTGGATGGGTTT GCTAGCCTTCACCAGCATCATAGGCTGTGCCTTTAAATGCAGTAACCTACACTGGCATTATAACTTAGCC AAGGTAAGGTCCATGTAGAACCTGTAGTTTTCTCATTATTTTATCAATTTTAAGTTTTTCTTCCTCAGAC ACTAGATTATTTATTTGAGTATAAATTCAGGCTCTTTAGGATTAATTATATGCTGACTTCCTTGGGAGAG TTTAAAATTATATGCTGAATTCCTTCGAAGAAATTGGCAGTGCAATTTCTATGGATTCCACAAGTTTATT CATTATAAAATACTAACAGCAACATAGACTCCTCAGTTTTAAGCTGCTGTGTGTGCTGTCTGAAACTGGC TTGTCAAGGGAATGCTCACCTTTTTTATCTCTGAGAAGCTGGGCAGGGAGATGAATGATGGACCTTTGGC AGATCTTCTCTGGACTTTGCAAGAGTGTAGCTAAACCACAAAGTAGTGTGACATTGACACTTCTCATTTG CTACTGTGACACCAAACAGTGCCATCACCCTAATGGTACAGAGTAAAGTTGGAGATAGACCTTAAGCTGC CAGATTTCAAGCTGGCATGTAACTTTTCATCTTCAGCAGTTTGCCTGGGTTCTGGTTACTGTGAGACAAA CAGCATTCCCTTTACAGGACTATGCAAGGGCTTTGTTAGTCTTCCAGATAAGGCCTCTGGCTTCCTCTGT AAGAGGAATGCTACTGCATTGGGAGTCTACTGTGGAGACTGTTCCCAATCCAAGTCTTTTGGGAACAGAG TGTAAAGTAGCAATGACCTCTTGGTGGAAGTGATGCCTTGGTGATCCCAGCACACACGTGCACTGTCTAT CAGAACCAGGGGCAGCAGTGAGAGGGTTTGGCAGTGACACTGGAGGTGAGCGTGACAGCACCATGGTAAC AAGAAGAATGGGTGTGTTTTAAACAAAGATGCAGTCAGTGTTTTGAGCTGGCTAGAATCTTACCCAGAAA CTAAACCTTAATTGATAAACTCTCACCATGGTTAAAATATTTGAGTGTCTGTGTAGAGATGCAAGAATAA TGAAGCTTCTAGCAAGACTACAGACTGCCCGAGAATCCACCATAGTTACCTCCTTAAAGGTTACTTCAGC TCTAAGTCATGTCAGTGCCTCTGCTGGAGTTTGTTGGTTTGTAGCTTCATATATGATTTTTCATGGACTA GAAAGACAAAGGCTCAGAAACCAACAGTGGACATCAGTGTGCAGCAGTAGCTAAAGACAGAGGCCAGTGA AAATATCCATGATTTAGTTTCTACTTGGCAGCTTTGAATAGCTTAGTCTTGTCACTTTCCCTCCCCTTAG AGAGGAGACGAAGCAGCTGAGAGGTTGTGTGCGGCCTTTATCCTCAGACAGGAGGTTCAGGAAACTGGGA GGACCTATCACAGGAATCCATATGGTGGCAGTTGGCTCTGTTTGAATGAGGCTGCTCCAGGTTTATACTG ATCATCTTCCCGGATGATCCAGCTTCAGGGCCAATACCTCTGAGCCTGTAACACAGGTGAGGAGGGGCAC ATTTCCCAAGTCAGCTCAGAGTCGGCTACAGTCTCTTTGGCCCTTTCTCAATAAAGTTGTGAGTCTTTTA GTTGTCTTTTGTTTAGATTTTAGTTAGAATGGATATTTCTGGTGACCAGCCTTGGTATGTAAAAGCTCCT ATTGGAAAAAAAAAAATGTATTTTGCTTTTATCTCATTGAAAATCAGCAGTCTTATTGTCCGAATTTGAG TAGAACACTTGGATGTTCAACACATCCTAAGGTGACCGTTGTAACTGTGTCTTTCAAGACTTTGAGGCTC AATGACTTGTAGGTGGTACTTGTTATTTAAGTATAAATCATAGTAAATATTGTAAAGTATATTTTTTAAA AGTACTGGTGTAAGGGGACTGGAGAGATGGCTCAGGGGTTAAGGGCACTGACTGTCCTGAGTTCAATTTC CAGCAAACACATGGTAGCTCGTAGCCATCTATAATGAGCTCTGTTGCCCTCATCTGGCTTGCAGATACAT ATGCAGGCAGAACACTACATATGTAATAAATAAATAAATAAATAAATATTTTTAAAAAGTACTTGTATAA AATAATAAATGGAATGTATAGTAATTTATTAGTGCTTGTCATGCACAAAACATTCCTTATACAGTTTTAA AGGATTGAAAAACATGATAGTATAAGACTTTTGTTGCTAAAGAACTTCTAATTAATCACATAGATTAAAA CATTAAGTATAGGCCATTGATTGTGCTTACATCTATAATCCCAGTACTTGGGGATGGGATGGAGTACTGA AGTAAGAGGAGGCTTGTTAGTTTAAGGCCAGCTTGAGCTAGAGACTTACCTCAAAAAGTCTGTAAATATG CCAGGAGGTGGTGAAACATTCCTTTAATCCCAGCACTTGGGAGGCAGATGCAAGTAGATCTCTGTGATTT CAAGGCCAGCCTGGTCTACAGAGTGAGTTCTAGGACAGCCAGGCTACACAGAGAAACCCTGTCTCAAAAT AAAATATAATAAAGTAAAATAAACAAATCTGTAAATAGAGCATTTTCATTACTGTCTCTCCAGAATTACT GTTATTACCAGTGCAATCTCGATACCCTGGAGACTGCCTCTTGGTGCAGATCAGTGACCCTCTCAGCCTT TCTGTAGCCCATCCCTGTTCCTTTGTGTTAGCTGCTGATCTGAAGAACACTTTCTACCAGGGAACCCAGT GGCCGCACTCAGGCAGGCAAATTTCACTTTCATTCCTGTCCACCATTTCTGTCTCTCCAGAGTTGTTATT AATTCTGTAGCAGCCTTCCTGCTCCCATTCTCCTTCCTCTCCCTGGGGACTCTGCCTCTTGATGCTGACC TCTGTACCCCTAGTTCTTTCCTGCTGACTCTCAGCCGTTCCTCTTAGTTCATCTCTATTGCTTTGTATCA TCCACTCCCTGGCAGAATCACTGGGGACCCCCAGAGCAGTGAGCAACAAGAACCAAAGAACAGACTACCC ACTCAACAAAGACCAAACCAGATATTTACCCCACGTAACACCTCAGACACTGTAACTCCAGTGACTGGAT CACAGCACAGAGACATAAACAAGAACAGTCAAGACAATATACCTCTTTTAGAAGTTAGTAACCATATTGT AATAGGCCCCAAGAAATCCAATTTAGCTGAATCATCAGGCAAGGACTTCAAAACAGCAATTATGGATATG TTCAAGGATCTTTTAAAAATTTTTATTTACACCCCAATTGCTGCACCCCTTCCTGGTCCTCATCTCACAC ACTCCCTCCCTGTGTATCCCCAAACCCTGGCATATCAAGTCTCTGCAGGGCTAGGTGCATCCTCTCCTGC TGAGGCCAGTACAAGGCAGCCCAATTAGGGGACCATATTTCTCAGACAGGCAAGAGCTTTAAGGATAGCC CCACTCTAGTTGTTGGGTGATCCACATGAAGAAAGAACTTCACATCTGCTACATATGTGCTGGGAGTCTA GGTCCAGCCTGTGCATGCTCTTTGGTTGGTGGTTCAGTCCCCATCCTTGTCCCCTCTCCTACCCAGTCCC CTCCCTCCATCTGTCTCCTATAACTGTTTTATTTCCCCTTCTAAGTGAAAGTCAATCATTCTCAATTGGG CCTTCCCTCCTGTTTAGCTTCTTTAGGTCTGTGGAGTAGAATGTGGGTATCCTGTACTATATGGCTAATA TCCACTTATAAGTGAGTGGATGCCATGCATGTATTACATACTTAATAAAGTTGTTAGGAGTTACCATATA AACTTTCTGTGTTGTATCCACGTTTAAATGTCTGATGGTGGCCAAAATGTTCTTTTAATTAAAAAATTAA CAATTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6410104 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410104 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19451 same embryo
	EMAGE:19449 same embryo
	EMAGE:19447 same embryo
	EMAGE:19450 same embryo
	EMAGE:19446 same embryo
	EurExpress:euxassay_005139 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source