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EMAGE:19454

Golga7 golgi autoantigen, golgin subfamily a, 7 ( MGI:1931029)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19454 EMAGE:19454 EMAGE:19454 EMAGE:19454 EMAGE:19454
"Pseudo-wholemount" of euxassay_005151. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005151_01 euxassay_005151_02 euxassay_005151_03 euxassay_005151_04
EMAGE:19454 EMAGE:19454 EMAGE:19454 EMAGE:19454 EMAGE:19454
euxassay_005151_05 euxassay_005151_06 euxassay_005151_07 euxassay_005151_08 euxassay_005151_09
EMAGE:19454 EMAGE:19454 EMAGE:19454 EMAGE:19454 EMAGE:19454
euxassay_005151_10 euxassay_005151_11 euxassay_005151_12 euxassay_005151_13 euxassay_005151_14
EMAGE:19454 EMAGE:19454 EMAGE:19454 EMAGE:19454 EMAGE:19454
euxassay_005151_15 euxassay_005151_16 euxassay_005151_17 euxassay_005151_18 euxassay_005151_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19454Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19454_wholemount_strong.wlz
19454_wholemount_moderate.wlz
19454_wholemount_weak.wlz
19454_wholemount_possible.wlz
19454_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19454_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8615
Entity Detected:Golga7, golgi autoantigen, golgin subfamily a, 7 ( MGI:1931029)
Sequence:sense strand is shown

>T8615
ATCGGCGAGGGGGGAGGCCTGGGGCGTCTTTGGGCGGTGGGTGGCTCCGAAGGGCTGGCGGGCCGGAGAG
CCGGCTCTAGGCCGGCCATCTGACCCCGTGTCTTGCCCGAACTCGCAGAGCGGCGGGTCCTGTCCTCGCC
ATGAGGCCGCAGCAGGCACCGGTGTCCGGGAAGGTGTTCATTCAGCGAGACTACAGCAGCGGCACGCGCT
GCCAGTTCCAGACCAAGTTCCCCGCGGAGCTGGAGAACAGGATTGACAGACAGCAGTTTGAAGAAACAGT
TCGAACTCTAAATAATCTTTATGCAGAAGCAGAGAAACTTGGGGGCCAGTCGTATCTTGAAGGCTGTTTG
GCTTGTTTAACAGCATACACCATCTTCTTATGCATGGAAACTCATTATGAGAAGGTCTTGAAGAAAGTCT
CCAAATACATTCAAGAACAGAATGAGAAGATATATGCTCCCCAAGGCCTCCTCCTAACAGACCCCATTGA
GAGAGGACTTCGAGTTATTGAAATCACCATCTATGAAGACAGAGGTGTGAGCAGTGGAAGATAAACCACA
GAGTTACAGGTCCTGCCTCCAGCTGGGACCTTCGTCTATCCACTGGCCGATGGCAGAGTGTCCCTACCTC
CTCTCCAGAGCGTTATTCTCTTGTCTCTGCTGCCAGAGCCACGGTGCCACACCTGGAGTGACCGCTCGTC
ACTCAGCATCCACTTTGTCTCCCAGTTGTGTAGGACTCTGTGATCTTTTGATTTGTTTCCAAGAAAATAA
AGGAAGCAATTCTCTTTATAAATTCAAAGCCATTTAAAACACAGGCAGTTGGCCAGCCCAAGACAGAGTC
GTTTTCTTACCAGATACCAGTGTCACTGGTTCTCCTTGTCCCTTGGGTCAGTTTCCTAGGTGGCTTTATG
ATCCCACAAGTCATGTCTTAGCTCAGTCCCTCCCCCAGATTAGTAGCAGGCTTTGAGGGTGTGTGTGCCT
GTGGTCCGGCTGACCCGCCGCCCGAGTCCCCAAGCTACCAGCCTTGACGTGCTTCAGATCTTCAGATTGG
TTCTGCTGTTCAATTTCAGAGGCTCCTTGAGAGAATAATATATGTTACTTGTTTTAGTGTTGCAGTTATT
ATTAAGGCAGTATTTATGCAATTCCGGTTGTTTCTTTGAAAGCAGTTCTGTTAATGTGCATTACCTCGAG
ATGTTGGGGAGATGTTGTGTGTGTTGCTTGTTCATTCCACAGATAAGTAAGCACAATGAGCAGACCCTCA
GCCATCAGCTCCACCTGCCCCAGCTCTGCCCGGTGTGGAAGAAGCAAGTGTCCTAAGCACCTAACTCTAG
TTTTGTTACAAGTATAACTTAGGAGAAAAAATTGGTAGAAATAATACAGTATGTTGCTAATTTGTAACCT
ACTTTAAACTCTGATTTATAGCCTCACATACAGTATTCAGGGCACAGAAATCTTTTGATTGACTCAAGTA
AGTTTTGGCTTCCGTGTAACTTCAAGTTAAAGTTAATTTTTTTCAGCTCTTCTTTATCTCTATACTCTCT
TAGTATCGGAATTTCTTATGTTCTTTTAGGAATTTGAGAGCACTGTGTTTGGGAGAAGTTAGGAAACATA
AGGAATGAATGAAGCAGTTGAACTTGGAGATACTGTAGGTGCTAATACTGGATCTAATCTTTCAGATTTA
ATTTGTTTTCTGGGCCTTGTAGTTACTCAGTAAACCCCTTAGATCTGTGTAATAATTTAATTGTATAACG
CTCATTTGTTCCTTTAAAGCCTATTCTAGTCTGTCTGCCTTTTAAACTTGTTGCAATAATGTCGCCAAGA
TATAAACACATTAGTAAAACTTTTCTTAAATAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5717934 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5717934 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19457 same embryo
 EMAGE:19455 same embryo
 EMAGE:19453 same embryo
 EMAGE:19452 same embryo
 EMAGE:19456 same embryo
 EurExpress:euxassay_005151 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS