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EMAGE:19481

Plekhj1 pleckstrin homology domain containing, family J member 1 ( MGI:1925920)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19481 EMAGE:19481 EMAGE:19481 EMAGE:19481 EMAGE:19481
"Pseudo-wholemount" of euxassay_004887. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004887_01 euxassay_004887_02 euxassay_004887_03 euxassay_004887_04
EMAGE:19481 EMAGE:19481 EMAGE:19481 EMAGE:19481 EMAGE:19481
euxassay_004887_05 euxassay_004887_06 euxassay_004887_07 euxassay_004887_08 euxassay_004887_09
EMAGE:19481 EMAGE:19481 EMAGE:19481 EMAGE:19481 EMAGE:19481
euxassay_004887_10 euxassay_004887_11 euxassay_004887_12 euxassay_004887_13 euxassay_004887_14
EMAGE:19481 EMAGE:19481 EMAGE:19481 EMAGE:19481 EMAGE:19481
euxassay_004887_15 euxassay_004887_16 euxassay_004887_17 euxassay_004887_18 euxassay_004887_19
EMAGE:19481 EMAGE:19481 EMAGE:19481 EMAGE:19481 EMAGE:19481
euxassay_004887_20 euxassay_004887_21 euxassay_004887_22 euxassay_004887_23 euxassay_004887_24
EMAGE:19481 EMAGE:19481
euxassay_004887_25 euxassay_004887_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19481Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19481_wholemount_strong.wlz
19481_wholemount_moderate.wlz
19481_wholemount_weak.wlz
19481_wholemount_possible.wlz
19481_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19481_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6879
Entity Detected:Plekhj1, pleckstrin homology domain containing, family J member 1 ( MGI:1925920)
Sequence:sense strand is shown

>T6879
GCGGCCGAGAGGCAGGGCGCGTGTTGGTTCGCGCGGATCCGTGCGGCGGGATGCGGTACAACGAGAAAGA
GTTGCAGGCGCTGTCCCGACAGCCGGCAGAGATGGCGGCCGAGCTGGGCATGCGGGGCCCCAAGAAGGGC
AGCGTGGCCAAGCGGCGACTGGTGAAGCTGGTGGTCAACTTCCTCTTTTACTTCCGCCCGGACGAGGCCG
AGCCCCTCGGAGCCCTGTTGCTGGAGCGCTGCAGAGTGGCCCAGGAAGAACCGGGCGGCTTCTCCATCAG
CTTCGTCGAAGACCTCAGCAGGAAATATCACTTTGAATGCTGTAGCCAAGAGCAGTGTCAGGACTGGATG
GAAGCTCTGCAGCGGGCCAGCTACGAATACATGCGACAGAGCCTCATCTTCTACAGGAATGAGATCAGGA
AGATGACTGGCAAGGACCCCCTGGAGCAGTTTGGCATCTCCGAGGAGGCCAGGTTCCAGCTGAATAGCCT
GCCTAAGGATGGGCGGACTACTTGTATTGGCTCCCAGTTAAATGTGTTGGACTGACCCTCTCTGGGCCCC
TGGCTACCCTGGGAAGTTTTTTTTTTTTTAATATAGGTTTTAATACATCTGTTCAAAAGGTCCAGAGGAG
GTGGGCAGGGTCTGTGGTCCTCGTTCTCCATGCCATCTCCCTGCCAGCACCCCTTGAGTGTCCACATCCA
GGGACCGAGCCTGGCACAGCTCCCCTATCCATTGCCAGATGAATGTATAATGCTAGATGAAGCTGTGAAC
CCCCCCCCCCCCTCCTGCTGATAGAGCAGGGCATGGGTGCTGGCCTCAGTGCTCAGCCTGCTCTTTAATA
AAGGTACCTCTTTTCTGAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3966305 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3966305 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19482 same embryo
 EMAGE:19484 same embryo
 EMAGE:19483 same embryo
 EMAGE:19485 same embryo
 EMAGE:19486 same embryo
 EurExpress:euxassay_004887 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS