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EMAGE:19508

Higd1b HIG1 domain family, member 1B ( MGI:1922939)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19508 EMAGE:19508 EMAGE:19508 EMAGE:19508 EMAGE:19508
"Pseudo-wholemount" of euxassay_004909. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004909_01 euxassay_004909_02 euxassay_004909_03 euxassay_004909_04
EMAGE:19508 EMAGE:19508 EMAGE:19508 EMAGE:19508 EMAGE:19508
euxassay_004909_05 euxassay_004909_06 euxassay_004909_07 euxassay_004909_08 euxassay_004909_09
EMAGE:19508 EMAGE:19508 EMAGE:19508 EMAGE:19508 EMAGE:19508
euxassay_004909_10 euxassay_004909_11 euxassay_004909_12 euxassay_004909_13 euxassay_004909_14
EMAGE:19508 EMAGE:19508 EMAGE:19508 EMAGE:19508 EMAGE:19508
euxassay_004909_15 euxassay_004909_16 euxassay_004909_17 euxassay_004909_18 euxassay_004909_19
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euxassay_004909_20 euxassay_004909_21 euxassay_004909_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19508Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19508_wholemount_strong.wlz
19508_wholemount_moderate.wlz
19508_wholemount_weak.wlz
19508_wholemount_possible.wlz
19508_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19508_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6886
Entity Detected:Higd1b, HIG1 domain family, member 1B ( MGI:1922939)
Sequence:sense strand is shown

>T6886
AACAGATCCATGACCAAGAGGGCCCCAAGATTCAAAGACTGTTCCAGTCTACTCTGGATTTTTCCAGCTG
TTTTTCTTGGCCAGAGGATCACACCCATCGGGATGCAAACCGAGAGCAGGAAGCAAGCGGAACAGTAAAG
TCAGATGAACCAGAGGAGAGGCAGATGTCACGGACACGAGGACGGTAGCTTCGAGCAGCCCAAACCCTTG
CAAGAGAACACCCAGAGGGGTATCTTTTCAAGTGCAGGTTGTGCTGGACATGGTCAGGACCTGAAGACTA
AAAGTCAGAGTCCTTATTGTGGGCTATCCTCTTCTAGAAGTGGGCTAAAGCCTAGGCACACAGCTGTTAA
CATCTGGATCCAGGATCATGTCTGCTAACAAGGGCTGGTGGGTGCCACCTGAGGGTGAGGACAACCTGTC
CAAGAAGTTCCTAAGGAAGACCAGGGAGTCTCCACTGGTGCCTATAGGTGTAGCAGGCTGCCTGGTGATA
GCAGCATACAGGATTTACCGGCTGAAGGCTCGGGGTTCTACCAAGCTCTCCATCCATCTGATTCACACCA
GAGTGGCGGCACAGGCGTGTGCTGTGGGGGCCATCATGCTTGGTGCCATGTATACAATGTACAGAGATTA
CATCAAGAGGGTGTCAGAGGATGCCGAGAAGTAGGACTCCAGAGGTGGCAGGATGGCCCAGTCCCCCGGC
TGTCTGCATGTTCTAATAAAGCTATGCTGAGGAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3967350 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3967350 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19506 same embryo
 EMAGE:19507 same embryo
 EMAGE:19505 same embryo
 EMAGE:19510 same embryo
 EMAGE:19509 same embryo
 EurExpress:euxassay_004909 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS