EMAGE mouse gene expression spatial database

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EMAGE:19526

Zfp768 zinc finger protein 768 ( MGI:2384582)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005040. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005040_01	euxassay_005040_02	euxassay_005040_03	euxassay_005040_04

euxassay_005040_05	euxassay_005040_06	euxassay_005040_07	euxassay_005040_08	euxassay_005040_09

euxassay_005040_10	euxassay_005040_11	euxassay_005040_12	euxassay_005040_13	euxassay_005040_14

euxassay_005040_15	euxassay_005040_16	euxassay_005040_17	euxassay_005040_18	euxassay_005040_19

euxassay_005040_20	euxassay_005040_21	euxassay_005040_22	euxassay_005040_23	euxassay_005040_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19526_wholemount_strong.wlz

19526_wholemount_moderate.wlz

19526_wholemount_weak.wlz

19526_wholemount_possible.wlz

19526_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19526_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7448
Entity Detected:	Zfp768, zinc finger protein 768 ( MGI:2384582)
Sequence:	sense strand is shown >T7448 CTCGGGGCTCCAGGCACTCGAACTGGGTCGTAGGATCGTCCGCCATCGCCACCGCCGCCGCACTGGGAAC TGGCAGGGATGGAGCGGGAGGCGTCGTCGTGGGGCCTGGAGTCCCGGGATGTGCACAGTCCCAACGCAGT GGGCAGCCCCGAAGGGTCTCTCAAAGATCCTGCAGGCAACACAAGTGAGAATGAAGAAGGGGAAATTTCT CAGCGAGAAGGCAATGGGGACTATGAAGTTGAAGAAATACCTTTTGGGCTTGAACCCCAAAGCCCAGAGT TTGAGCCACAAAGCCCAGAATTTGAATCCCAGAGCCCCAGGTTTGAGCCTGAAAGCCCAGGGTTTGAATC CCGAAGCCCTGGGTTTGTGCCCCCAAGCCCTGAGTTTGCACCCAGAAGTCCTGAATCAGATCCTCAGAGC CCGGAGTTTGAATCCCAGAGTCCTAAGTATGAGCCCCGAAGCCCTGGCTGCCATCCCAGGAGCCCTGGCT GTGAACCTGGGAGTCCTAGGTATGAACCCAAAAGCCCTGGGTACGGTTCAAAGAGCCCCGAATTTGAATC TCAAAGTCCAGGGTATGAATCTCAGAGCCCCGGGTATGAACCGCAGAACTCTGGAGATGGCGTTCAGAAT TCTGAGTTCAAAACCCATAGCCCTGAGTTTGAAACTCAAAGTTCCAAATTCCAGGAAGGTGCAGAGATGC CTCTGAGCCCCGAGGAAAAGAATCCCTTGAGCATCTCCCTAGGAGTCCACCCCCTGGATTCCTTTACTCA AGGGTTTGGGGAGCAGCCCACAGGAGCGCTGCCTCCATTTGACATGCCTTCGGGGGCTCTGCTGGCTGCA CCCCAGTTTGAGATGCTCCAGAATCCACTGAATCTGACAGGAACCCTGCGTGGGCCAGGCCGTAGGGGTG GCCGGGCCAGGGGTGGGCAAGGCCCTCGACCTAACATCTGTGGCATCTGCGGGAAGAGCTTTGGACGAGG CTCGACCCTGATCCAGCACCAGCGCATCCATACCGGCGAGAAGCCTTACAAATGTGAGGTTTGCAGCAAG GCCTTTTCCCAGAGCTCTGACCTCATCAAACACCAGCGCACGCATACAGGCGAGCGGCCCTACAAGTGTC CCAGGTGCGGCAAGGCCTTCGCTGATAGCTCTTACCTACTCCGCCACCAGCGTACCCACTCTGGCCAGAA GCCCTACAAATGTCCACACTGTGGGAAGGCCTTTGGCGACAGCTCCTACCTCCTGCGACACCAGCGCACC CACAGCCACGAGAGGCCCTACAGCTGCCCCGAGTGTGGCAAGTGCTATAGCCAGAACTCGTCCCTGCGCA GTCACCAAAGGGTGCACACTGGGCAACGGCCTTTCAGCTGTGGCATCTGTGGCAAAAGCTTCTCCCAACG GTCAGCACTTATCCCCCACGCCCGCAGCCACGCCCGTGAAAAGCCCTTCAAGTGCCCAGAGTGCGGCAAG CGCTTCGGCCAGAGCTCAGTTCTGGCCATCCATGCCCGCACCCACCTGCCAGGCCGCACGTACAGCTGCC CCGATTGTGGCAAGACCTTCAACCGCTCCTCTACACTCATTCAGCACCAGCGTTCCCACACAGGCGAGAG GCCGTACCGCTGCGCTGTGTGTGGCAAGGGCTTCTGTCGCTCCTCCACGCTGCTACAGCACCATCGCGTG CATAGTGGGGAGCGACCTTATAAGTGTGATGACTGTGGAAAGGCCTTCTCGCAGAGCTCCGACCTCATCC GCCACCAGCGGACCCACGCAGCCGGCCGCCGTTAACCAGTCACGGGTGGGGAGGTGGTGTGCAGAAGAGA TGGGGTCAAGGAATTTATGGATAGAGGAAAAGCTGGTGGAGAATAGAAGGACGAAATATTCTAGAAGATG GGTCAGGGTGCAGGAAGTCACATGCCCTCAAGAAGTATGAGAGTTGGGCTATGGAGGGGTTGGAGGGAGG CCAGACAGGCACCAGGAGCAAGTCTGCCTCAGCAGCAGTGAGCTCTTTATCTGGGAGGCCCTGTTCTGGC AAAGTACTAGATGGGGAGGGGGGAGGGCAAGGGAGTGTCAGTTAGAGTAGGGTAGCTTAGATTGGTAGCT GCTGAGACCCTTGTGAAACCAGGAAGAGGGCAAAGGGTAGGCAGTGGGGAGTGAGGCCCAAGGGTAGGGT CTGGATCTCAGAGTAAACTGCCTTGTCTTCCTCAGGTTTTGGAGCCCTGTGAGTTCAATAAACCTTTTAT GTGGTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4235387 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4235387 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19525 same embryo
	EMAGE:19523 same embryo
	EMAGE:19522 same embryo
	EMAGE:19524 same embryo
	EurExpress:euxassay_005040 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source