EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19546

Pfpl pore forming protein-like ( MGI:1860266)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004989. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004989_01	euxassay_004989_02	euxassay_004989_03	euxassay_004989_04

euxassay_004989_05	euxassay_004989_06	euxassay_004989_07	euxassay_004989_08	euxassay_004989_09

euxassay_004989_10	euxassay_004989_11	euxassay_004989_12	euxassay_004989_13	euxassay_004989_14

euxassay_004989_15	euxassay_004989_16	euxassay_004989_17	euxassay_004989_18	euxassay_004989_19

euxassay_004989_20	euxassay_004989_21	euxassay_004989_22	euxassay_004989_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19546_wholemount_strong.wlz

19546_wholemount_moderate.wlz

19546_wholemount_weak.wlz

19546_wholemount_possible.wlz

19546_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19546_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7992
Entity Detected:	Pfpl, pore forming protein-like ( MGI:1860266)
Sequence:	sense strand is shown >T7992 TTCATTTTCGGCAAAAGAATCTGGCACTGGAGACTGACAAGAGAACAGAACAGTGAGCCAAGATTGTAAC AAGCCTGAGAGAGTGAAGAAAGCACTACAGCAGACTCTTCAGGCTCTCTGGTGGATGTTTAGGACTGGTG TGTGTCTGGGACTACCTGCACCATGAACAGCTTCATTGTCACAGTCCTTATCTGGACCACAGTGGCATAT GCTGAAGAGGAGAAGGCACTTAAAGAGATCCATGAGTATGGTTTCCAAAAGTGCCAGAATGCTCTGAATC TACCTGTTTTGGAAGTGCTACCTGGAGGAGGCTGGGATAATCTGAGGAATATGGACATGGGGCAAGTGAT GGACTTGACATACAACAACTGTAAGACCACAGAAGACGGACAGTACATCATCCCTGATGATGTCTTTACT ATTCCTCAGAAAGAGAGCAACCTGGAGATGAACTCAGAAATCCTGGATTCCTGGGAGAATTACCGAAGCA CTACTTCCTTTTCCATTAACTTGAATCTCGATTATCGTCCCAGAGTCAACGGCAAGTTCTCTTCTGAGTT CCAAAGGATAAAGACCACTCAAGTGAGAGACCAGGCTGTAACCACACGAGCTCAGGTGAGAAATTTGGTC TACACAGTGAAAGCCAACCCAAATGCAGAACTCAACTTGGGGTTTAAGAAGGAGCTCATGGAAATCTGTG ACCGTCTAGAGAAAAACCAGACGAAGATGGCCACCTACCTGGCAGAACTCTTGATCCTCAACTATGGCAC ACACATAATCACTAGTGTGGATGCTGGGGCTACACTGGTTCAGGAGGATCATGTAAAGTCCTCCTATCTT AAGAACAATAAGGGAAACCGTGCTGCTGTGGCTGCATCTGCTGGATTTACTTTCGCAAAGGTTGTGAACT TCAAAATAGAGACAGGCTTTGAGTATCAAAATAACTTGGCCACAGGCTATCTTCAAAACAGAACCGGTTC TAGGGTTCAGAGTATTGGAGGAATTCCCTTCTACCCAGGGATGACCATCGAAACCTGGCAGAAGGGCATC ATTAACCACCTGGTGGCAGTGGACCGTGCTGGCTTGCCTCTGCATTTCTTTATCAAACCTGAAAAGCTTC CTGCTTTTCCACGCCACTTGGTGGAGCAACTGGCAAAGACAGTGGAAACTGCTGCGGAAAGCTATTACAA CTTTAATACATACCCAGGCTGCACAAATATCAACTCCCCCAACTTTGATTTTCAAGCCAATACAGATGAT GGCTCTTGTGATGGTAAACTGGTCAACGCTCCCTTTGGAGGGGTTTATCAGTTGTGCCGTCAATTATCAG GGCATGATTACGATGACATGTGCCTAGATTTCCATCAGAAGAATCCACTTACTGGTGATTTCTCTTGTCC GCCTGACTACACCCCTGTCCATCTGCTATCTCAGACCCATGAGAAGGGTTATACTGGAATGGAATGTAGA GATAAATGTATCCTCAAGATATTCTGTCGTACATCGTGTAAAGATGAGTTCAGAGTGGCCAAGGCTGAAG TTAGCTCTTATTGGTGTGCGGCCAGTAGCCAAGTACCAGAAAACTCAGGAATTCTCTTTGGAGGAGTCTT CACTGACAAGAGCATCAACCCTATGACAAATGAACCATCGTGCCCAACCAGTTACTTTCCTCTGAAGCTG TTTGAGAACGTCAAGGTATGTGTTTCTCTGGATCATGAGTTGGGGCCCAAATATTCAGTCCCCTTTGGTG GGTTTTTTAGCTGCACAAAAGGGAACCCCTTGTATGGTTCTTTCACATCTGGAGACTTAGAGAAATCATT TCTGCCAAAGTGTCCCGGGGGCTTCAGCCAACAGCTCGCTGCGATCATTGATGGATGCCAAGTGTCCTAC TGTGTCAAGGCTGGAGTCTTTACACGAGCATCTCTGGCTCCTGCTAGGCTCCCACCTTACACTCAGCTAC CTATGAGTCAGCTTTCCACTGACTCAGTTGAGATGACAGGGGAGAGTCCCATATCCTGGGTTAAGGACCC CTATACCCTGCAATGGAACCTAGAGGAGTCATCAAAAAGTGGTAGACTATCAGGAGGATCAACTGCTGCA ATCACAATTGTGGTTATCCTAGCATTAGGAGTTTTAACTGCTATGGCCATCTATGGCAACCGGAGGTTTA AGAAGAATAAACCCAATGGACTTCCAAGACAACAGGCATTGCTTCCTTTTCCAAGAGTTAGAGTCTTTCC TGATGGAGTGCAGGATCCAAATCCAGCTTAATTCTTCTTTAAAGAAACAGTTCCTCTCATCTCCAGACAC AGGTTCAAGCTCCTCTTTCAGTCCTTTCTCCTCTACCTCTGCCTTCCTAAGAGCTAAAGCAGAAGTCGCA ACCGAAAGAAGGTTTTTTATAACTTTGTGGTTATGTTTATGACTATACATTGGGAATTAAAGGATCTGTG CAAATAAGGCAGGGGAAGGAAAACTTGCAACCCAGTCAGCAAAAACAAGCCCAGGTTCTCAGCCATTGTC GGAATGCTCATTCCCACACAAGCCACTGGTGTCTTCACCTGAATCTCTGCATGAAGGAACAACTGGGTGG AGAGCAACAAGAACTTTGGGATGAAGCTGCTCTTATTGTGAAGTTGGCAAAGGTTTTCCAATTTGTGAGC AGAACACTTTGCCTTCCCTGTATCTTGTTTTGTATTTTAGCCTCTTAAAGACAACCCAAACTCTGAGAAC ATGCAGATGGTCCAGTTCAACCAGTGTTGTGGAGACTCCAAAACTCTGTACTGATGACTGCGTCATCTCC CTGTGTGCCTGAAGGGCACAGAGTTCAGAACAAGTCCACTTTCTGTCTGCTGCACAGTTAGAATTTACCT TGTTCAACCTACTTTTAGAGCAATTTTAACTTGTGAACTTCCCATGCAATGTCCAAGGCAAAAGGGTGGT GGGGAACTGGAAATAAATCAAGAAATGTAAAAACTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30020114 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30020114 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19545 same embryo
	EMAGE:19547 same embryo
	EMAGE:19544 same embryo
	EMAGE:19548 same embryo
	EurExpress:euxassay_004989 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source