EMAGE mouse gene expression spatial database

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EMAGE:19555

Sephs2 selenophosphate synthetase 2 ( MGI:108388)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002086. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002086_01	euxassay_002086_02	euxassay_002086_03	euxassay_002086_04

euxassay_002086_05	euxassay_002086_06	euxassay_002086_07	euxassay_002086_08	euxassay_002086_09

euxassay_002086_10	euxassay_002086_11	euxassay_002086_12	euxassay_002086_13	euxassay_002086_14

euxassay_002086_15	euxassay_002086_16	euxassay_002086_17	euxassay_002086_18	euxassay_002086_19

euxassay_002086_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19555_wholemount_strong.wlz

19555_wholemount_moderate.wlz

19555_wholemount_weak.wlz

19555_wholemount_possible.wlz

19555_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19555_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 07 08 09 10

foregut-midgut junction

strong

regional

strong expression: see section 06 07 08 09

hindgut

strong

regional

strong expression: see section 06 07 08 09 10

rectum

weak

regional

weak expression: see section 12

midgut

strong

regional

strong expression: see section 05 06 07 08 09 10

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5470
Entity Detected:	Sephs2, selenophosphate synthetase 2 ( MGI:108388)
Sequence:	sense strand is shown >T5470 GGTCCGGCTCGCGTGGTTGATCATCTCCTGGCGTAACCTTGGCCCGCTGTGGCTGGGAGACTCATCTGCA GGTATCTGGGCCTTCTGGTCCGCACGGCCTCCCGGGCGAGCGGCGCGATGGCGGAAGCGGCGGCGGCGGG CGCCAGCGGAGAAACCATGGCGGCGCTAGTGGCCGCAGAAGGTTCCTTGGGCCCGGCGGGCTGGTCTGCC GGCCGGAGTTTCTCCAACTACCGGCCGTTCGAGCCCCAGACACTGGGCTTCAGCCCGAGCTGGCGGCTGA CGAGCTTCTCCGGCATGAAGGGCTGAGGCTGCAAGGTCCCCCAGGAGACCCTGCTCAAACTCCTGGAGGG ACTGACGCGGCCCGCGCTGCAGCCCCCGCTTACCTCGGGTCTGGTCGGGGGCCAGGAAGAGACGGTGCAG GAAGGGGGCCTGTCCACCAGGCCCGGCCCCGGCTCAGCCTTCCCCTCGCTGAGCATTGGCATGGACTCCT GCGTCATCCCCCTGAGGCACGGAGGCCTGTCGCTGGTGCAGACCACCGACTTCTTTTACCCCTTGGTGGA AGATCCCTATATGATGGGGCGCATAGCTTGTGCCAATGTGCTCAGTGACCTCTATGCCATGGGTATCACT GAGTGTGACAACATGTTGATGTTACTCAGTGTGAGCCAGAGCATGAGTGAAAAGGAACGAGAGAAGGTGA CACCGCTCATGATCAAAGGCTTTCGTGACGCTGCGGAGGAGGGAGGCACTGCAGTGACCGGTGGACAGAC AGTGGTCAACCCGTGGATTATCATCGGTGGCGTTGCCACGGTGGTGTGTCAGCAAAATGAATTCATAATG CCTGATAGTGCCGTGGTAGGAGATGTGCTGGTATTAACCAAACCTTTAGGAACCCAGGTTGCTGCCAATG CCCACCAATGGCTGGATAATCCTGAGAAATGGAATAAAATCAAGATGGTGGTTTCCAGAGAGGAAGTAGA GTTAGCCTATCAGGAAGCTATGTTCAATATGGCTACTCTAAACAGGACTGCTGCTGGCTTGATGCACACT TTTAATGCCCACGCAGCCACGGATATCACAGGCTTTGGCATATTAGGACACTCTCAGAACCTGGCAAAAC AGCAAAAAAATGAAGTGTCCTTTGTCATACATAATCTGCCAATAATTGCCAAGATGGCTGCGATCAGCAA AGCCAGTGGGCGCTTTGGCCTCCTCCAAGGAACGTCAGCTGAAACCTCTGGGGGATTACTGATTTGTCTG CCAAGAGAGCAGGCGGCCCGCTTTTGTTCGGAAATCAAATCTTCCAAGTACGGAGAGGGTCACCAAGCTT GGATCGTTGGCATCGTGGAGAAGGGAAACCGGACAGCCCGGATCATTGACAAGCCTCGCGTTATTGAAGT TCTACCTCGGGGAGCCTCTGCTGCTGCTGCTGCTGCTCCTGATAATTCCAACGCAGCCTCTGAGCCTAGT TCTTGAAATGGAATAGCGGTTGTTGGGAACTCGGAGCCATTCTACCCGCTCAGGGACTGCTGGCCAGGGT TGATTTTAAGACCTTTCCAAAGGCTGCTTGCATGGTTCCTCCAGGCCCATCCAAAGCTTCCTGTATGTGC ATCCAGGCCTGTGAGTAATGGCGCTGCGGATGTGTGTTCATCTGTTGAGAGCATGAGGAGCAAAAACCCG TTTCCCAAAGCAAGAGGAGGCTATTTCAGTTTTAGGGATTTTTTTTTTTTTTTTTTTTTGCACTGAGTTG ATTCATTTCTGCACAGGGAGTAAAGATTATTAAGATTACATATGAGAAAAGTAAACCTGCAACATGAAAA ATTATTTGGACCAATATATTGATAAATCTAAATTGTTAGGAGAACTCTTACTGATTTATTGTCAAATTTG TTATTAATTTTTTTCTGAGAAACTGCCTCTTTTCCTGTTCTGGACAAGAGTTGAGCAGCTTGTCCGACAG GAAAGGAAGACTAGCCACCTGACTTGGTCTCTGATAATGATGTCTCTCCCTCTAACTCCCAGTAAGGACT GGGAGAGGCTGAACAAACCTCAGAGCCAGGTGTCGGTGGCCATTGAATCTTACACTGAAACTTCTGGAGA TTTAATTAATAAAGAGAATTTCTTACAGTAACTAAATAAAAGGGACTTTGTTGGAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5374184 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5374184 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19557 same embryo
	EMAGE:19556 same embryo
	EMAGE:19560 same embryo
	EMAGE:19558 same embryo
	EMAGE:19559 same embryo
	EurExpress:euxassay_002086 same experiment
	MGI:4827955 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source