EMAGE mouse gene expression spatial database

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EMAGE:19563

Oraov1 oral cancer overexpressed 1 ( MGI:1919534)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006092. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006092_01	euxassay_006092_02	euxassay_006092_03	euxassay_006092_04

euxassay_006092_05	euxassay_006092_06	euxassay_006092_07	euxassay_006092_08	euxassay_006092_09

euxassay_006092_10	euxassay_006092_11	euxassay_006092_12	euxassay_006092_13	euxassay_006092_14

euxassay_006092_15	euxassay_006092_16	euxassay_006092_17	euxassay_006092_18	euxassay_006092_19

euxassay_006092_20	euxassay_006092_21	euxassay_006092_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19563_wholemount_strong.wlz

19563_wholemount_moderate.wlz

19563_wholemount_weak.wlz

19563_wholemount_possible.wlz

19563_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19563_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rest of cerebellum mantle layer

moderate

single cell

moderate expression: see section 04 20 21 weak expression: see section 05 06 07 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6536
Entity Detected:	Oraov1, oral cancer overexpressed 1 ( MGI:1919534)
Sequence:	sense strand is shown >T6536 GGCGTTTGTGATCGCGTCTCGCCAGTTGGCGATTACTACACAGAATGAAAGGAATGCGAGGGCCGGGGGA GAGCCGAGACACCGCCATGGCCGTGGAGCAGGACATATTTGACGCCGTCGTGATGGCGGATGAGAGATTC CATGGGGAGGGATATCAGGAAGGCTATGAAGAAGGCAGTAGCTTGGGGATCGTTGAAGGAAAGCGGTATG GCATGGTACATGGAGCCAAGATTGGGTCTGAGATTGGATGCTACCGGGGTTTTGCTCTTGCATGGAAGTG TCTCCTACACAGTGGCGCTGGTGAAAAAGACAGCAGGAAGATGAAGGTGGTGGAGGCTCTGATTGCGCTG CTGCAGGACTTTCCTTACGATGATCCCACTTATGAGAAGCTCCATGAAGACCTGGACAGAATCAGAGGGA AGTTCAGGCAGCTGTGCTCACTGCTCAACGTGCAGCCAGACTTCAAGGTGACTCCAGGAGGCTCTGGACT TGCGTTTTGAGGACCCACAGAAGAGCAGATGTCTGCACATTAAGTGTCACAGTGATTAAAGGCAAGCCTG AGAGGAATCCATTGTTCTGTGGGAGGACCAGCTTGGCCTCTTCCCTGAGTCTCTGCCTAGGTGGCTTACC TGACCACCCTGCCTTCTGCACAGGGGCAACACACTCAAAAGACCGCTTGTCACTGGCCAGAGTGAGTGGC CCTGGGTAGGGTTACCCTGAGGGTGGGACTCACAGAGGCAGGCCGATGTGAAATGGGTCATGGGAAGCCG TGATAAGATCTTTGCTGCCTGCTGAGCCTCTGCTGGCCATGCTTGGGCCTCCTTGTTTCACTCCATCCCT CTGGCAGCCCCAGAGGTGGAGGATACCTTATGGCTTAGAAGTGATAAAATAGACCCATCTAGTCTAAGGG CCTGGTGGCAGAGCTGGCCCCCACCCCAGGTCCATGCCCTGAAGTACCACATAGTGCCTCTGCCTCCAAC ATGCGCTGTCATTGCCAACACCACTGGAGGACCTGATTCCTGCTATCTGCACAGGACTCAGGCTCCACTG GACTTGGTTTGCCTCTGGATGCCCAGCTGAGAAAACAAGGGTGACGAGACAAGGGAGGAAGCTGAAGCAG GGCCTTCATGCTGCTCCTAACAGGTGTGGGGTAAGCTCTATACATTAAGGAGTGGCAATGGAGCTGGGTG CTGGTGGTGCACGCCTTTAATCCCAGTGCTCAAGAGATAGTAGGCAGATCTCTCAGTTTGAGGTCAGCCT AGTCTACAAAGTGAATTCAAAACCAAACAAACAAAAAGTGGCATTAGGGGAACTATTGGAAACCAAAGCA GGACTAATTGGTGGCCGCCACTGTGAATGTGTAGGGCTTGCCTGGTGACACTGTACTTCCTTTAGCAACA GCTTGTGTGTCTGGCATGGTATTTACAGGCAGATATAAGACGAGCTGGCAGGAGTGGCACAGTGCTCTCA GGCATTTCATAGAGAGAATATCTTTGTGTTGTATGGATGCCTCACCTGTGGCAAAAAGCCTATGGCCTAT GGCTTAGGCAGGAAATAGGCGGAACATCTGGGAGAGAGAAAGCATTCTGGGATAGAGCCAGGTGGGAGAT CTACCAGGGAGGATGTGAGGAGATGGACACATGGTACCTGAGCACAGGTAACCAGCCAGCTGGCAGAATG CAGGTTAAAATAAATGGGTTATCTTCAGTTATAATCTAGTCAGAATAGAGCCTAGCTGTATGTCCGAAGG ATTTGTAAATATGTTTTGAGTCTGAGGCTTGGGGCACCATGCACCTGGCCTCCTGGGCCTGGCGGCTACC TCTCAGGTATTCAGCACCACTCTCTCAGGTGCCCAGGATCAGGGACTCTTCGGTGTGAGAACTGAGCTGC TGTTATCACCATATTTTACTTTCCTATTATATTCAGAAAAAAGAATAAATGGTGTATTTTAAAGCAGTAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3591291 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3591291 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19566 same embryo
	EMAGE:19561 same embryo
	EMAGE:19562 same embryo
	EMAGE:19564 same embryo
	EMAGE:19565 same embryo
	EurExpress:euxassay_006092 same experiment
	MGI:4826981 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source