EMAGE mouse gene expression spatial database

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EMAGE:19587

Asb13 ankyrin repeat and SOCS box-containing 13 ( MGI:2145525)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005117. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005117_01	euxassay_005117_02	euxassay_005117_03	euxassay_005117_04

euxassay_005117_05	euxassay_005117_06	euxassay_005117_07	euxassay_005117_08	euxassay_005117_09

euxassay_005117_10	euxassay_005117_11	euxassay_005117_12	euxassay_005117_13	euxassay_005117_14

euxassay_005117_15	euxassay_005117_16	euxassay_005117_17	euxassay_005117_18	euxassay_005117_19

euxassay_005117_20	euxassay_005117_21	euxassay_005117_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19587_wholemount_strong.wlz

19587_wholemount_moderate.wlz

19587_wholemount_weak.wlz

19587_wholemount_possible.wlz

19587_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19587_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7530
Entity Detected:	Asb13, ankyrin repeat and SOCS box-containing 13 ( MGI:2145525)
Sequence:	sense strand is shown >T7530 CCCAGAGCTCTGAGTCACAAGGCCGGTAGACAGGGGAGCCCAGCGGGCGAGCCGCATGGAGCCGCGCGCC GGGGACGGCTGCTTCCTGGGAGATGTGGGTTTCTGGGTGGAGCGCACGCCCGTTCATGAGGCGGCTCAGC GGGGTGAGAGCCTCCAGCTTCAGCAGCTGATTGACAGTGGAGCATGTGTTAACCAGGTCACTGTGGACTC GATCACACCCCTTCATGCAGCAAGTCTGCAGGGCCAGGCCCAGTGTGTGCAGCTGCTGCTAGCAGCTGGT GCCCAGGTGGACGCACGCAACATTGACGGCAGCACCCCTCTTTGTGATGCCTGTGCCTCAGGCAGCATTG AGTGTGTGAAGCTCTTGCTGTCCTATGGGGCGAAAGTCAACCCTCCTTTGTACACAGCATCCCCGCTACA CGAGGCCTGCATGAGTGGGAGTTCTGAATGCGTGAGGCTTCTTATTGATGTTGGGGCCAACCTGGAAGCC CACGATTGCCATTTTGGGACGCCTTTACATGTTGCCTGTGCCCGGGAGCATTTGGACTGTGTCAAAGTGT TGCTCAATGCAGGGGCCAATGTGAACGCAGCCAAACTTCATGAGACTGCGCTTCATCATGCAGCCAAGGT AAAGAACGTTGACCTCATCGAGATGCTCATAGAGTTTGGAGGCAACATCTATGCCCGGGACAACCGGGGC AAGAAGCCCTCGGACTACACGTGGAGCAGCAGCGCCCCGGCCAAGTGCTTTGAGTACTATGAAAAGACAC CTCTTAGCCTCTCCCAGCTCTGCAGGGTGAGCCTGAGGAAGGCCACCGGTGTCCGAGGACTAGAGAAAGT CGCCAAGTTGAACATCCCTCCCCGGCTCATCGACTACCTTTCCTACAACTGAGCTGCAGGCAGGGGCCGG AAAGGCTGATGGCTGGCCCAGTTATCACCTTGCTTTGCCTAGCCCTGGGCATGGTATGAGTTCTGCCTGT TTCTCTGAAACACTAACATTGCTGTAGATTGGTTGATGCTCCTTCATGTTCCTTCTGGTTGTCCTTTTTG ATGTCTTCTTCTGAACCCAGAGAAGATCTCTGAAGCCCCAGAAGGCCAGTGTCTGGCATCACAGTTGTGG ATGTAACCCTTGCTAGTCTCCTGGAACAAATGTAGTCATTCTAGGACAGGCTCAGTTCTCTGTGTTTCTC TTTTCACCGGCCACCCTCAAATAGAGACAGTTCAAGGTGCACAGGGGAATCTGGGGGCTCAGAATGCTGA TGTCGATGGTTTTTATCTGGGTGCCATGCCACAGAAGCATCAGGTTCTAAGCATTTGACCCTGTGGACTG GGACACTACTCTGCTGGCATCGTGCCATTTGGATGGTTTTTCTGGGGCAGCAGCTGGCACTTTCCAAGGT CTACCTGTACCCAGGTATATCACTCAGGGGCTTTACAGATCAGAGTTTGTACCCCCACATCAAGCACTGT TTTTGGGGGGGGGGATGACTGTCTCCAATATTAGGACTCAAATCTGTTAAAAAAAGTTTATGTTGGGAGC CTGGAGAGATAGCTTGGGGCTAGAGTTTAGTTTCCAGAACCCACATAAGGCAGCTTACAATGGCCCATTA CTTCAGCTCCAGGGGATCCAACACCCTCTTCTGGCCTCCTCAAGCACTCAGACATGTATGTGTATACACA CACACACACACACACACACACACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATAAATA AAATAAAATCTTTTAAAAAGTTGGAGACCCTTTAGATTATATATAGATGTGTATATATACATATATACAC ATACATTCATGTATATGCTGTAACAAGATTTGCAATGCAAACAATATAAACAATAGCTAATATGAAAGTG CAAAACTTTATCCAGATGGGAATGAGATTCTTTAGCCAAAGCAGAAAAAGGGGTCACAATTTCTTCCTAT AGTAATAACAATGAGTCCTGTACATTGTGGGTGAAATACTAAGTCTTGCATTCAGACTGAATTTTCCGGA TTGACATTCTATATTTTAACAATACTGATAATACCCCACATACAATAGAAAGAATTAGAGAATTTTCCAG TAGTCATTCTCTACTGAGAAAAGCCACCTTCCTTCTGTCAGTCTCTGGTACAAGTTCTGAGGAAATCCAT GTTTTTGCATGGAATAGTAATAGCAGTTATTTTCAGAAAGGCACTGGAGTCTGCCTCTTTCGTCTTTGTA ACAGAATCTTGCCTCTAGCAATAAAACCCAATAACAGCCCATCTGCTATATCTTTTGGTAGAGAATAAGG TTTTCTCACCATTATTGTCCAATAAAAGTGGTTGGATGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239996 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239996 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19586 same embryo
	EMAGE:19585 same embryo
	EMAGE:19588 same embryo
	EMAGE:19584 same embryo
	EurExpress:euxassay_005117 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source