EMAGE mouse gene expression spatial database

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EMAGE:19591

Cyp4a31 cytochrome P450, family 4, subfamily a, polypeptide 31 ( MGI:3028580)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006145. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006145_01	euxassay_006145_02	euxassay_006145_03	euxassay_006145_04

euxassay_006145_05	euxassay_006145_06	euxassay_006145_07	euxassay_006145_08	euxassay_006145_09

euxassay_006145_10	euxassay_006145_11	euxassay_006145_12	euxassay_006145_13	euxassay_006145_14

euxassay_006145_15	euxassay_006145_16	euxassay_006145_17	euxassay_006145_18	euxassay_006145_19

euxassay_006145_20	euxassay_006145_21	euxassay_006145_22	euxassay_006145_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19591_wholemount_strong.wlz

19591_wholemount_moderate.wlz

19591_wholemount_weak.wlz

19591_wholemount_possible.wlz

19591_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19591_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7501
Entity Detected:	Cyp4a31, cytochrome P450, family 4, subfamily a, polypeptide 31 ( MGI:3028580)
Sequence:	sense strand is shown >T7501 ACCTCAACAAAGGTGAACTATGAGTGTCTCTGCTCTAAGCCTGACCCGATTTGCAGGCAGCCTCTCTGGC TTCCTCCAAGTGGCCTCTGTGTTCTGTCTGCTCCTGCTGCTGGTGAAAGCTGTCCAGGTCTACCTGCACA GGAAATGGCTACTCAAGGCTCTCCAGCAGTTCCCATCACCGCCCTTTCACTGGTTCTTTGGGCATGAGCA GTTCAAAGGTGACCAAGAACTACAGGAAATTGTGTCATGCATAGAGAACTTCCCAAGTGCCTTTCCTCGA TGGTTCTGGGGGAGCAAGGCCTACTTAACTGTCTATGACCCTGACTACATGAAAGTGATTCTGGGGCGAT CAGGGTATGGTTTGCTCCTGTTAAACGGACAGTCATGGTTCCAGCACCGGAAGATGCTAACCCCAGCCTT CCACTATGACATTCTGAAAACCTATGTGAAAAACATGGCTGACTCCATTCGATTGATGCTAGACAAATGG GAACGGCTGGCTGGTCAGGACTCCTCTATAGAGATCTTTCAACACATCTCTTTAATGACCCTAGACACTG TCATGAAGTGTGCCTTCAGCCACAAGGGCAGTGTTCAGGTAGATGGAAATTACAAGACCTACCTCCAGGC CATTGGGGACTTGAATAACCTAGTTCACTCTCGTGTGAGGAATATGTTTCATCAGAATGATACTATCTAC AAACTTTCTTCCAATGGCCGCTTGTCCAACCAAGCTTGTCAACTTGCCCATGATCACACAGATGGAGTGA TCAAGATGAGAAAGGATCAGCTGCAGGATGAGGGAGAGCTGGAAAATATCAAGAAGAAGAGACGTTTGGA TTTTCTGGATATCCTCTTATTTGCCAGAATGGAGAATGAGGACAGCATGTCTGACAAGGACCTTCGTGCT GAGGTGGACACATTCATGATCGAGGGCCATGACACCACAGCCAGTGGAGTCTCCTGGATCTTCTATGCTC TGGCCACACACCCTGAGCACCAACAGAGATGCAGAGAGGAAGTTCAGAGTCTACTGGGGGATGGATCCTC TATCACCTGGGATCACCTAGACCAGATTCCCTATACCACAATGTGCATCAAGGAGGCCCTAAGGCTCTAC CCACCTGTCCCAAGCATTGGCAGAGAACTCAGTACATCTGTCACCTTTCCTGATGGATGTTCTTTACCTA AAGGTGTCCAGGTCACACTCTCCATTTATGGTCTCCACCACAACCCGAAGGTGTGGCCAAACCCAGAGGT TTGCACCAGACTCTCCCCGACACAGCCACTCATTCCTGCCCTTCTCAGGAGGAGCCAGGAACTGCATTGG GAAACAATTTGCTATGAGTGAGCTGAAGGTGATTGTGGCCCTGACACTGCTCCGCTTCGAACTACTGCCA GATCCTACCAGGGTCCCCATGCCCTTAGCCCGATTTGTGCTGAAGTCCAAAAATGGGATCTACCTACATC TCAAGAAGGTCCACTGATTCTGATAAGGACAAGGACAGACTCTATAAACATACTGCCTGACATCTTGTCC TGCCACCATCTTCCTCTCTGTACATCTTTCTTCACACCTATTTGTTCCCTGCCTCCTGCACAATCTCTCT CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACACACACA CACACACACACACACACCTTGCTCTAGATTCTCCATTTGTCTTTCTGTCTCTGCTACCCACATCTGACTG TACTCCTGATAATGAACCTCCTCTCTCCTCCAGTCTTCTCTCCTTGCCTCTCACTTTCCACCTATCTTTT CCCTACATGACTTCTTCATTTTACAAATAATCAATTGTCATAACCTTGGTGGTCTGAAATAATTGAAAGT TATTATCTTACAGTTGTGGAGCTCCAATGTCTGAGAAGAGTTTCACTGGAGAAAGCTAAGTTGTTGGTGT GACCTACCTTTTTCAGAAGAATACTCCAGAGAGTCCTCTCATCCTCTGTTCAGTATAGATCATCATTCCT TGGCACTGGAATATTCCCCTGCTTCAAAGCCAGCACCCTCCTCATCCCCTCTCTTCTAATGTCCTTACCT TTTACAGATGCTTATGACATCATTAAGATCCCTAAAAGCTCCAAGATAGTTTTCCCATCTAAATGTATCT TATTCAATCTAACCTACTAAGTTCCTTCTGAATTATGAGGAATCATTCAATATGTACCATGGTCTCCAAA ATCCAAGGCCTGAACATCATTGTGAAGACATTATTCAGCTTAGTATCATTTTCACATGACAGTTACCTAA TGTGTTCATCTAAATCTACCCTGCATAGTCTCTTTCTACCTGATTAATCCTCACACGAGTTTCTCCATCT TCCCTCCTTTCTGCAATAAAGTTCCCAGTGTCATGCACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4237837 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4237837 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19590 same embryo
	EMAGE:19592 same embryo
	EMAGE:19589 same embryo
	EurExpress:euxassay_006145 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source