EMAGE mouse gene expression spatial database

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EMAGE:19597

Plac1 placental specific protein 1 ( MGI:1926287)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004991. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004991_01	euxassay_004991_02	euxassay_004991_03	euxassay_004991_04

euxassay_004991_05	euxassay_004991_06	euxassay_004991_07	euxassay_004991_08	euxassay_004991_09

euxassay_004991_10	euxassay_004991_11	euxassay_004991_12	euxassay_004991_13	euxassay_004991_14

euxassay_004991_15	euxassay_004991_16	euxassay_004991_17	euxassay_004991_18	euxassay_004991_19

euxassay_004991_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19597_wholemount_strong.wlz

19597_wholemount_moderate.wlz

19597_wholemount_weak.wlz

19597_wholemount_possible.wlz

19597_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19597_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

spotted

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 weak expression: see section 05 19

body-wall mesenchyme

moderate

spotted

moderate expression: see section 05 06 07 08 09 12 13 17 18 19 weak expression: see section 04

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8027
Entity Detected:	Plac1, placental specific protein 1 ( MGI:1926287)
Sequence:	sense strand is shown >T8027 AAGGGACCTGCCTCATCTTCTCGCTACCCCTTCCCTCGTGCTCCACACGGAGAGAACAGAACTAACAGAA TTCTCAGCCCAAGATTTCTTTTTTTAACTGCCTTCAGCAGTGAGCACAAAGCCACGTTTCAAAGGAGACT GACAAATTATCCCCAGCTGCCAGAAGGAGAATCCTTCCTGGACGCCTTCTTGTCTCCTGTGAGTCTTCAC CTAACCTGCTGCAAGAATGAACCTTCGCAAGTTCCTGGGAGGCACTGTCTTAGTCGCCTTCATGCTCTTC AGCTACTCGGAGCAAAACCAAGTGAATGTGCTGTGCTCGACAGATTGGTTCATGGTCACCGTTCACCCCT TCTTGCTGAATAATGATGTGTATGTTCATTTTTACGAGGTGCACCTGGGCCTTGGTTGCCCTCCCAATCA TGTTCACCCACGTTTCTACCAGTTTCACTACCGCGTTACCGAATGTGGCATCCGCATCAAGGCTGTCTCT CCAGATGTCGTCATCTATAGCTCTGAGATTCACTATGCTTCCAAGGGCTCATCAACGAAATATGTGATTC CCGTGTCCTGTGCTGCCCCTCGCCGGTCCCCTTGGCTCACTAAGCCCTACTCCGCGAAAGCACCTAGCAA CAACATGGGTGCGACTCCGAAGAATGATACTTCCTACCACGTGTTCACCTTGCCAGAGCCTAGCGGACAA CCCAACTGCAGCTGCCCACCTTATGTCTACAATCAAAAGAGCATGTAAACCCTCCATCATGGGACAGAGT CTTTGGAGGTCCTTCTTGTGTAGTCATCTGACTGTGCAAATGTTTCTGGGGACTAGTGTGTTCACTCAGA TGATCTGAGGAAACCCATATAGTGCCCAGATGTAAGGGCTTCTGAAAATTCTGTTTCTAAAAATGAATAT ATAGCATTTTCTACTGTCAGCCAAATAGATGCCATGATAGCTCTTTTAGGGGAATTTTGGAAAGGGTCAA TTCATATATAGCTGATTGTAACTTTTTTTTTTACTATTTTTGTATCTTTTAAAACAATAATAAAATTCTG ATTGGCAATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30017089 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30017089 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19596 same embryo
	EMAGE:19595 same embryo
	EMAGE:19593 same embryo
	EMAGE:19594 same embryo
	EurExpress:euxassay_004991 same experiment
	MGI:4827247 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source