EMAGE mouse gene expression spatial database

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EMAGE:19615

Khk ketohexokinase ( MGI:1096353)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005122. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005122_01	euxassay_005122_02	euxassay_005122_03	euxassay_005122_04

euxassay_005122_05	euxassay_005122_06	euxassay_005122_07	euxassay_005122_08	euxassay_005122_09

euxassay_005122_10	euxassay_005122_11	euxassay_005122_12	euxassay_005122_13	euxassay_005122_14

euxassay_005122_15	euxassay_005122_16	euxassay_005122_17	euxassay_005122_18	euxassay_005122_19

euxassay_005122_20	euxassay_005122_21	euxassay_005122_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19615_wholemount_strong.wlz

19615_wholemount_moderate.wlz

19615_wholemount_weak.wlz

19615_wholemount_possible.wlz

19615_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19615_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7462
Entity Detected:	Khk, ketohexokinase ( MGI:1096353)
Sequence:	sense strand is shown >T7462 GGAAGCTTGGGGAGCAGCCTCATGGAAGAGAAGCAGATCCTGTGCGTGGGGCTGGTGGTGCTGGACATCA TCAATGTGGTGGACAAATACCCAGAGGAAGACACGGATCGCAGGTGCCTGTCCCAGAGATGGCAGCGTGG AGGCAACGCATCCAACTCCTGCACTGTCCTTTCCTTGCTTGGAGCCCGCTGTGCCTTCATGGGCTCTTTG GCCCCTGGCCACGTTGCCGACTTCCTGGTGGCTGACTTCAGGCAGAGGGGCGTGGATGTGTCTCAAGTGA CTTGGCAGAGCCAGGGAGATACCCCTTGCTCTTGCTGCATCGTCAACAACTCCAATGGCTCCCGTACCAT TATACTCTACGACACGAACCTGCCAGATGTGTCTGCTAAGGACTTTGAGAAGGTCGATCTGACCCGGTTC AAGTGGATCCACATTGAGGGCCGGAATGCATCGGAACAGGTGAAGATGCTGCAGCGGATAGAGGAGCACA ATGCCAAGCAGCCTCTGCCACAGAAGGTCCGGGTGTCGGTGGAGATAGAGAAGCCCCGTGAGGAGCTCTT CCAGTTGTTTAGCTATGGTGAGGTGGTGTTTGTCAGCAAAGATGTGGCCAAGCACCTGGGGTTCCAGTCA GCAGTGGAGGCCCTGAGGGGCTTGTACAGTCGAGTGAAGAAAGGGGCTACGCTTGTCTGTGCCTGGGCTG AGGAGGGTGCCGATGCCCTGGGCCCCGATGGTCAGCTGCTCCACTCAGATGCCTTCCCACCGCCCCGAGT AGTAGACACTCTTGGGGCTGGAGACACCTTCAATGCCTCTGTCATCTTCAGCCTCTCGAAGGGAAACAGC ATGCAAGAGGCCCTGAGATTCGGGTGCCAGGTGGCTGGCAAGAAGTGTGGCTTGCAGGGGTTTGATGGCA TTGTGTGAGAGGCAAGCGGCACCTGCTCGATACCTCAGAGGCTGGCACCATGCCTGCCACTGCCTTCTCT ACTTCCTCCAGCTTAGCATCCAGCTGCCATTCCCCGGCAGGTGTGGGATGTGGGACAGCCTCTGTCTGTG TCTGCGTCTCTGTATACCTATCTCCTCTCTGCAGATACCTGGAGCAAATAAATCTTCCCCTGAGCCAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4235560 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4235560 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19614 same embryo
	EMAGE:19616 same embryo
	EMAGE:19618 same embryo
	EMAGE:19617 same embryo
	EurExpress:euxassay_005122 same experiment
	MGI:4825749 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source