EMAGE mouse gene expression spatial database

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EMAGE:19679

Plekha6 pleckstrin homology domain containing, family A member 6 ( MGI:2388662)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006131. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006131_01	euxassay_006131_02	euxassay_006131_03	euxassay_006131_04

euxassay_006131_05	euxassay_006131_06	euxassay_006131_07	euxassay_006131_08	euxassay_006131_09

euxassay_006131_10	euxassay_006131_11	euxassay_006131_12	euxassay_006131_13	euxassay_006131_14

euxassay_006131_15	euxassay_006131_16	euxassay_006131_17	euxassay_006131_18	euxassay_006131_19

euxassay_006131_20	euxassay_006131_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19679_wholemount_strong.wlz

19679_wholemount_moderate.wlz

19679_wholemount_weak.wlz

19679_wholemount_possible.wlz

19679_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19679_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8554
Entity Detected:	Plekha6, pleckstrin homology domain containing, family A member 6 ( MGI:2388662)
Sequence:	sense strand is shown >T8554 GTTAGAGGCAGCAGAGACTAGGGAGCGCACTGAGCTGCCACAATGAAAGACGCAACACATTTCGACATCC AGTGACTGGCCAGATCCCAGAGGAGAACAAAAAGTTTGACTTGAAAACATCGGCCTTGGACATGTCCAAC AAAACAGGTGGGAAACGTTCTGCTACCATCAACAGCGACATAGCCAACCACAACATGGTGTCTGAGGTTC CTCCAGAGCGGCCCAACATCCGGGCGACTCGAACTTCCCGAAAAGCCATTGCCTTTGGTAAACGCGCACA CTCCATGAAGCGGAACCCCAATGCACCTGTTACCAAGGCGGGCTGGCTCTATAAGCAGGCCAGCTCCGGG GTGAAGCAGTGGAATAAGCGTTGGTTTGTCCTGGTGGATCGCTGCCTCTTTTACTACAAAGATGAGAAGC AGGAGAGCATCCTGGGCAGCATCCCCCTGCTGAGCTTCCGGGTGGCGGCTGTGCAGCCTTCAGACAACAT CAGTCGGAAGCACACGTTTAAGGCAGAGCACGCTGGAGTCCGCACCTACTTCTTCAGTGCCGAGAGCCCC GAGGAGCAGGAGGCCTGGATCCAGGCCATGGGCGAGGCTGCTCGGGTCCAGATCCCTCCAGCCCAGAAGT CAGTGCCTCAGCCTGTGCGTCACAGCCTCGAGAAGCCAGACTCAGAGAACATCCCACCCAGCAAACACCA TCAGCAGCCACCTCACAACAATCTCACCAAGCTGGAGCCTGAGGCCAAGACTCGAGGGGAGGGCGACGGC AGGGGCTGTGAGAAGGCCGAGCGCAGGCCTGAGAGGCCTGAAGTCAAGAAAGAGACTTTGGTAAAAGCCA ATGGCCTCCCGTCTGGACCTGAGACAGCCTCAGAGCCTGGCAGCCCTTACCCCGATGGCCCGAGGGTTCC AGGAGGTGGGGAACACCCTGCTCAGCCCAATGGCTGGCAGTATAGCTCCCCAAGCCGACCAGGGAGCACA GCTTTTCCACCTCATGATGGGGACTCTGGGGGACAGCGGCGGAGCTTTCCACCACGTACTGACCCTGACA AAATTGCCCAGCGCAAAAGCTCTATGAACCAGCTTCAGCAGTGGGTGAACCTGCGCCGAGGGGTGCCCCC ACCCGAAGACCTTCGGAGCCCTTCAAGGTTCTACCCTATGCCCCGCCGGGTCCCTGACTATTACAATCCC TACTCCTCCCAGTATCCTGATGATTACCAGTACTACCCACCAGGGGTGCGGCCCGACAGCATCTGCTCCA TGCCAGCCTACGATAGGATTAGTCCACCCTGGGCCCTGGAGGACAAGCGCCACTCCTTCCGGAATGGGGG TGGACCCACTTACCAGCTTCATGAGTGGAAGGAGTCTACCAGCTACGGGCGGCAGGATGGCACCGTTTGG ATCCCCAGCCCCTCCCGACAGCCAGTCTTTTATGACGAGCTGGATGCCGCCTCTGGCTCCTTGCGCCGCC TGTCCTTGCAGCCCCGGTCCCACTCTGTGCCCCGCTCGCCCAGCCAGGGCTCCTACAGCCGTGCCCGCAT CTACTCCCCAGTGCGCTCACCTAGCGCCCGTTTTGATCGGCTGCCACCACGCAGTGAGGACATCTATGCA GATCCCGCTGCCTACGTGATGAGGCGATCTATCAGCTCCCCAAAGTATGATTACCTGGGAGACAGGCGGC CAGTCCCTGCAGGACTGTTCCCCTACAACTACCCATCATCCCCCACGGTCCACGATAAGATGGATGAACT GTTAGATCTTCAGTTGCAAAGAAACCTAGAGTATTTGGACCAGCAGATGAGCGAGAGCGAGACTCTCATC AGCATGGTGAACCGAATGGTGGAGAACTCCTCTCCCAGGGCCCACCTTTTCATGCAAGTCCCTGCATACC CAGAGGTGTTCCGGGATGGCCTCCACACCTTCAAGCTAAACGAGCAAGACACGGATAAGCTGCTGGGCAA GCTGTGTGAGCAGAACAAGGTAGTAAGGGAACAGGAGCGGCTGGTGCAGCAGCTGCGGGCAGAGAAGGAG AGCCTAGAAAGTGCCTTGATGGGAACCCACCAGGAGCTGGAGATGTTTGGGAGCCAGCCCGCCTACCCAG AGAAGCTGCTGCACAAGAAGGAGTCTCTGCAGAACCAACTTATCAACATCCGGGTGGAGCTGTCACAGGC GACCACGGCACTGACCAACAGTACAGTGGTGTATGAGAACCTCGAATCGGAGGTCTCTGCCCTGCATGAC GAACTCTGGGAGCAGCTCAACTTGGATATCCAGAATGAGGTGCTCAATCGGCAAATCCAAAAGGAGATCT GGAGGATCCAGGATGTGATGGAAGGGCTGAGGAAGAACAACCCCTCTCGGGGCACTGACACAGCCAAGCA CAGAGGAGGACTTGGACCGTCAGCCACCTACAGCTCCAACAGCCCAGCCAGCCCTCTCAGCTCTGCCAGC CTTACCAGTCCCCTGAGCCCCTTTTCAATGGTGTCTGGCTCTCAGGGTTCTCCCACTAAGCCAGGGTCCA GTGAGGAACCTGGCCCACCTCGGCCACCCCTCCCCAAAGCCTACGTCCCCCTGGAATCTCCTCCCACCGT CCCTCCACTCCCCAATGAGAGCCGCTTCTGGCCATACCCCAACTCCCCTTCCTGGCATCGAAGTGGCGAG ACAGCCAAGGGCCAGCCCAAAACAGGCTATGAGACAAGCAAGAAAGACCCGAGCCAGACATCACCCCTGG GTACCCCTAGAGATATCAACCTGGTACCCACCAGACAAGAGGTGGAGGCAGAGAAGCAGGCAGCTCTCAA CAAAGTTGGCATTGTACCCCCTCGGACCAAATCTCCTGCTGAGGAGGAGTTGACACCATCAGCAGTGGTG AGAAGGACCACGAATGGACTCACCAATGGCCTCTCCTCACGGCAAGAGCGCCCCAAGAGTGCTGTGTTCT CTGGAGAGGGCAAGGTGAAAATGAGTGTCGAGGAGCAGATGGACCGGATGAGGCGGCATCAGAGCGGTTC CATGAAGGAAAAGCGGAGGAGCCTACAGCTCCCAGCCAGCCCAGCCCCTGAGCCCAGCACCCGGCCTGCC TACAAAGTGGTGCGCCGTCACCGCAGCATCCATGAAGTAGACATCTCCAACCTGGAGGCAGCCCTGAGAG CCGAGGAACCTGGTGGTCAGGCATATGAGACACCACGGGAGGAGATCGCGAGGCTCCGCAAGATGGAGCT GGAGCCTCAGCACTACGATGTGGACATCAGTAAGGAGCTCTCTACTCCAGACAAAGTCCTCATCCCTGAA CGGTACATTGACCTGGAACCTGACACTCCCTTGAGCCCGGAGGAGTTGAAGGAGAAGCAGAAGAAGGTGG AGAGGATCAAGACGCTCATTGCCAAATCCAGTATGCAGAACGTGGTGCCCATCGGCGAGGGGGACTCTGT GGACGTGCCCCAGGACTCAGAGAGCCAGCTGCAGGAGCAGGAGAAGCGGATTGAAATCTCCTGTGCCCTG GCGACCGAGGCCTCCCGCAGGGGCCGCATGCTGTCTGTGCAGTGTGCCACCCCCAGCCCTCCCACCTCCC CTGCTTCCCCGACTCCACCAGTCAACCCCCTCTCGTCTGACCGCCCACGGGGCGCCGACAGCAGCCATAC CATGCGGGTCTGAGCTCTGACTGCAAGCCCTGGCTGAGGCCAATGCTGTGAAGCTCCACAGAGCCACCTT CTGATAGCATCCATTGCACACCTGGGGCTCTGGCTTCTCACCCTGGCCTCCTGCCCTTGCACCCACCATG GGAACACGTCAGAGAGCCAGGCTGGGGACCGGGGCTGCTTCATAAAGGAACATGGATGCCTTCAAGTTCA CATCTGCTGCCCTTGCCCTGAAGCCTGCACTGTCATGTTATGGTTCTAAGGCAAGACCCGGGCATGGCAA GGCCAGGATGGCGTCCTCTCTGATGCCCCTGTCACGGGGAGCTCAAGTGCAGTTCTGGATGGATTGTGTG GCCCTCCTGCACCATCCCCCGTGGAGTCCATGTGCTGGTGGGAAGCTCATGCTATGGGTGAGGGCTAGAA GTGAAGACAAGACAGACTCCATCCCTTGGAACCCGTACAACACAGCGAGAGGCCAGGTCTTGCCATCACC TTCCTCCCATTCAGTCCCAGCTGCCTCAGCGATGCCCAAGGCTTTGGCACGGCTCTGCTGATGGGTTTCC CAGAGTTCACTGGAGGCCAGCTACCCTGCTTGAGCCAAAGAAGACGATGAGTTCTAGGGAGAGGCTCCTG GGCTCCCAGAGGGGTCAAGTGTGTGACAGAGAGACGACAGCAGGTCTGCACAGTGTCTGAGGGCAAGTTG GAAGCAAGGAGCAAGATGGAAGAGAAAAGAGGCTTAGAGAGTGAAGGAAGAGAAGGCAGACGCTTTTCAC AAGCAACAGGGATGTAAAGAAGGAGGGAAATGGGAAGGGAGAATAGAAATGGCTTCCCTAGTGTGGAGCC TTAGGTCAGTGCCAAGCAGAGGGGCTGTCACCTCTGTACCTTCACGTCTTCCTCGGGAGCAGGAGGCGCC AGGAGGACTCATGCCAGGCACATGCCAGCTCCAACTGAGGTGCTTGGTAGCAAGGTATGAGGTAAGGGGT TGTTAGAGTGCTATAGCCTGTGAGATGGTCCTATCTGTGTCAAGGCCTGCTGTCTCTCTCCCAGGGTCAT AGGCAGAGAGAAGACGGTCTCATATGAAGTCTGTCAGCCTTGGGGCCTTACCTAGCCAGTTTAAACCCGG AAAGTACTGTGGGCTGACTGAGGTTTGCCCTCGGAGGAGGAATGAGGAATTAACTGTGAGGCCAAGTTCT AGGTCCTTCCTTCTCATCTCAGGCATCTAGAGCAGGGCCAGATGCTTTCCTCCACCCCACCTGCCCAGGG AGGACAGGACAGGGAGAGACCCTAGCAGAGCAGAATCTTCCTCTAGCCCACCTACCGTGCGTGAATGTAG CCAGACAGCAGCAAAGGAAGGCTAGCTTCAGACACCAAGCCACCAGACCTGCTCTCCACACATTTTTGCC CAGAGACTTCAGCCTGAACATCAGTGGCCCAGGAAACAACTGCATCAGCTCCCATCAATCCATCACCACT CCGTCATGGTCGGGACAGTTACTGGTTCATATGCAAGTAAAGATGACAATTCTTTCAACAAATACTAGTG AAGCACTCTCTGTGTATCAGGCACTGTTCTAGGTGCTTAGGATATTGTCTTGCTTCTGAGGGACTCTGGA TCGGGAGGATTCCACCTGTTTTTGCCTCTCTCTGGCTTCTGGAAAGTGCTCCTTCATGACATCCCCTATC TGCATAGTTCAAGCACCCCAAATCTGCCCCTTCCTCCTTAAAGACTGGTAGATGGGATCAGCTCCCAGGT TACCCTCTCTCCCCATACCCTCTTCCAAAAGAAACAAAATGTTAGGGCTTTCCCCGTCGTTGTCTCTCCT TTTTTTTAAAAAGTGGTATTTTTTAGAAATACATGTGGAATACCAAGAAATGTCTTTGCCTCCCCACCAC TCTCACCTACATTTCATAAAGCTGGCTCTTTATGTTGCTTTACATGGCCTTAATATATGTTTTATGGAAG TGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5709381 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5709381 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19680 same embryo
	EMAGE:19678 same embryo
	EMAGE:19677 same embryo
	EMAGE:19681 same embryo
	EurExpress:euxassay_006131 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source