EMAGE mouse gene expression spatial database

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EMAGE:19682

Ermap erythroblast membrane-associated protein ( MGI:1349816)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005148. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005148_01	euxassay_005148_02	euxassay_005148_03	euxassay_005148_04

euxassay_005148_05	euxassay_005148_06	euxassay_005148_07	euxassay_005148_08	euxassay_005148_09

euxassay_005148_10	euxassay_005148_11	euxassay_005148_12	euxassay_005148_13	euxassay_005148_14

euxassay_005148_15	euxassay_005148_16	euxassay_005148_17	euxassay_005148_18	euxassay_005148_19

euxassay_005148_20	euxassay_005148_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19682_wholemount_strong.wlz

19682_wholemount_moderate.wlz

19682_wholemount_weak.wlz

19682_wholemount_possible.wlz

19682_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19682_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8827
Entity Detected:	Ermap, erythroblast membrane-associated protein ( MGI:1349816)
Sequence:	sense strand is shown >T8827 AGAATATGAACTTTTTGCAGACCCATCTCTTGAGATCTTCCTGGCACTCTTGCCCGATGTCAGACCACCC TGCCTGTGGGGAGCCCAGTGTTCCCAGGTAGGAGGCCCCAGCTCTGCCGCTAAAGGACACAATCTCCACC TCAAAGGTCTGTGTTGGAATGCTGAAGCGGCTAAAGAAACATGTGGTGGCGTGGAAAATGTGTGTAATGC CACACTCACGGAAGATGTCAGTTCATATGGAGAGACCAAGTCCCTGTGGTTCCTGGCTTGTCGGCTGTCT CTTCACCATAGCTGTCTTCCAGCCGCCTGTTCAGGTGTTAGGGGATGCTGGCAAGGTCTACATAGCCCCT CTTAGGGACACAGCCAACTTGCCCTGCCCTCTCTTCCTCTGGCCTAACATGGTACTCAGCGAGATGAGGT GGTATCGGCCCGGACACCTACCTCGCACCCAGGCTGTCCACGTGTTCCGGGACGGGCAGGACAGAGATGA AGACCTGATGCCAGAATATAAGGGCAGGACGGCACTGGTGAGGGATGCCCACAAGGAAAGCTACATCCTG CAAATCAGTAATGTGAGGCTCGAGGACCGAGGGCTATACCAGTGCCAGGTCTGGGTCGGAAACTCAAGTC GAGAAGACAACGTGACCCTGCAGGTGGCAGGTTCTGATCCCTACATCCACGTGAAGGGCTATGATGCTGG GTGGATCGAGCTGCTGTGTCAATCAGTGGGATGGTTCCCAAAGCCGTGGACTGAGTGGAGAGACACTACG GGTAGAGCGCTACTCTCCCTTTCAGAGGTTCACTCCCTGGATGAAAATGGGCTGTTCCGAACAGCAGTGT CCAGCAGAATCAGGGACAATGCCCTGGGAAATGTGTCTTGCACTATCCATAATGAGGCCCTTGGCCAAGA GAAGACGACGGCCATGATTATTGGAGCCCCAGAACGAGGAAGTCTGTCCTCTCCAGCGGTGGCTCTTTCT GTGGTCTTGCCCGTCTTGGGGCTCCTCATCCTCCTTGGCATCTGGCTCATTTGTAAACAAAAAAAATCAA AAGAGAAGCTGCTGTATGAGCAAGCGATGGAGGTAGAAAATCTTCTGGAAGACCACGCCAAAGAAAAAGG AAGACTCCACAAAGCCCTCAAGAAACTCCGGAGTGAACTGAAGTTGAAAAGAGCGGCGGCCAATGCAGGC TGGAGAAGAGCCCGGCTACATTTTGTGGCAGTGACCCTGGACCCTGACACGGCACATCCCAAACTCATCC TGTCTGAGGATCGGAGATGTGTGAGGCTCGGCGACAGGAAGCGGCCTGTTCCTGACAACCCAGAGAGGTT TGATTTTGTTGTCAGCGTCCTGGGCTCCGAGTATTTCACAACCGGCTGTCACTACTGGGAAGTGTACGTG GGCGAGAAGACCAAATGGATTCTTGGAGTGTGTAGTGAGTCTGTGAGCAGGAAAGGGAAGGTCACGGCCT CGCCTGCCAATGGCCACTGGCTTGTGCGACAGAGCCGTGGGAATGAGTATGAAGCTCTCACGTCCCCACA GACCTCCTTCCGCCTCAAAGAGTCCCCGAAGTGTGTGGGGATTTTCCTGGACTATGAAGCCGGCATCATC TCCTTCTACAACGTGACTGATAAGTCCCACATCTTCACTTTCACCCACAGTTTCTCGAGCCCACTTCGCC CTTTCTTTGAACCTTGTCTTCACGATGAGGGGAAAAACACGGCCCCTCTAATCATTTGTACTGAGCTTCA GAAATCAGAGGAATCGATTGTCCCCAAACAAGAAGGAAAAGACCGTGCTAATGGAGACGTGTCCCTGAAG ATGAACCCATCCTTACTGTCCCCTCAGGGTTCTGAGCTATTTCTGCTTAATGATACCTGGCCCTCTAACC TTGGCCCTGCCCTTAAGGGCCTCAAAGTTCCTTCTTTGTAGGGCTGTGCCCTGTGCTCCACTCTCGTGAG CATCCGGCCTGGCACAGGCTCCTGGCCCAGCATCTGTTTCTCCAGTACCTCATCTCCTTGACCCCAGTCC AGAGCCTTCTGTGGTTTCTCTTCTGCTTTTCTCCCAGGGCTCGGTTCTGAACTCTGCCTTGTTCTTGAGA CATCCCCATTGCCCTGGAAGATGACTTTTTGAAATCAACAAAATGCACTCGGGCCAGGTTTAGGAGGAGA CCTTGGATGTAATGCAGGGGGACTCCGTTCAGGCCAGGGGAAAGGGCTCTCTCCCTGAGGGAACCTTGGC ACCAGCTTCTCTTTGTCAGCCTGATCAGGGGTTATCAGGTGTGAACTTACAGCCCCCAGACCCAAATGTC TTCCACCAGAGACTGTTTTCTTTTCCCCCAGTCAGGCCTCTAACTCGCCACGCCAGCTTTTTACAAGTAT GTATCTTGAGACTCACACATCCTTTCTTCTCTGTTCAGGGTTAGAGATGGAGGGGTAAATACTCCATGGG TTTCTGTTTTCTTCAGGCTGTTTACAGCAGAGTTTCGAGACACCCAGAGCTGTTTTCGACAGCTAATTTG AGATGGAGACATCCAGAATGCACATGGTTTCAAAAATTAAGTTACCTACATCATGACAGGAACCAGAATC CCAAGAAACCTGAACACAGGCTGGTAGGAACAGCTGTAACCAAAGCAATCCAGCTGTGCAGCCCTGAGCA GATGAGTTACTCTGCAGTCTGTGGCTATGGCCCTTCTGAAAACTTCCTGACATTCAGAGAGGCCCAGTGA GAAAAAGGTGGCAGTTTGAACTACTCACGTATACCCACAAGACTATTAGATTTGACAACAGCCTCCAGGT CAGAGATTATTGAATTCAAGAAAGTTGGTCTTGTTTTAAATAAGACACCTTACTGCCAGGTATGGTGGTA CACATCTGTGATCCCAGGTGGATTATTGGAAGATATATCTGTGTAAATGATAGACCAGACTGGTCTCTGT AGTAAGTTCTAGGACAGCCAGGACTTTGTGGAGAAACCTTGTCTCAATGCCATGTCCTCAAAATGAGATA AGATATCATATTTGGAAGATTATAAGGTGAGACAACAAAATAATGAATTCAGGGCTGAAAAGATGGTTCA GCGATTAAGAGCTCATTCTGATCTTGCAGAGGACCTGGGTTTGCTTTTCTAGCACCCATATTGGGCAGTT CACAACCACCTGTAATTCCATATCTAGAGGATGAGACTTCAAGCCTTCAAAGGCACCTGCAACTCGCTGC ACATGCTACACATCAACACACACACACAGACTTTTTTTATTAGATATTTTCTTTATTTACATTTCAAATG CTATCCCCTTTCCTAGTTTCCCTCTGAAAACCCCTTATCCTCTTCCCCCTCCCCCTGCTCCCCAACCCAC CCACTCCCACTTCCCGGCCCAGGAATTCCCCTATACTGGGGCACAGAACCTTCACAGGACCAAAAAACCT CTCCTCCCATTGATGACCAACTAGGCCATCCTCTGCTACATATGCAGCTAGAGACACGAGTCCCTCCTTG TGTTTTCTTTGATTGGTGATTTAGTCCCAGGGAGCTCTGTGGCTACTGGTTAGTTCATATTGATGTTCCT CCTATGGGGCTGCAAACCCCTTCAGCTCCTTGGGTACTTTTTCTCTGGCTCCTTCATTGGGGACCCTGAC ACACAGGCTTTTTAAATTAAATACTTAAATAATGAATCCAAGTTTTGGATGTGGATTGGGGAAAGAAGGC AAGGCACTTTCAAGTCATACCCACATATGTGTTTCGGCCTGTTTGTGGAGTTATTAATTACAGATTTGCA GATGGGAAGGCAGCTGGACTCTACAGGAACTAGATCTGCTACAGGCTCTTTCCAATAGAGCCTATATTAG TGTTCCCTAGAGAAAGAGAACCAACTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGCGCACACACAT GCAGACACAGTAAGAGATAGAGACAGAAAATAAATACGAGACTGAAGTTGGCAGGTCAGAGTACTAGAAA GAGACAAGTTTTAGTTGCAGTGTAAGGTAGGGAAAGAGTTGATATCCGTCTAAAGCCCGACAGGCAGCGA GACTCTTCTAAGGTCTTCCACTGGTCAGATGACGACTCCCACTGGTTGGATGACTCCCACTGGTCAGATG ACTCCCACTGGACTTAAGGAGGGTGACTGCTTTACTCAGGCTACTGATTAAATGCTAATCTCGTCCAAGC TCCTCTCACAGAGACACCAAATGTGATGTCTGACCAAGTGTCTGGGACCTTTGTGACGGGTCAAAGTGAT ATGTAAAATTTGCCATTACAGTACCCAAAAAGAATATTTTGTTTGTCTTTGTACAGTTATGAAATATACC CTTTAATACTTATTGCTTGGTATGTTGTCTTCATTATGAAAGAGGCTGTGGAGAAAACACATTACGGGGT TGGTTTCTGAATAGCTCAGCCACAATTCAAGCAAACTTTAGACATCATTCATAATAAACCCAGTTTTACT CCTGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6410069 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410069 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19686 same embryo
	EMAGE:19683 same embryo
	EMAGE:19684 same embryo
	EMAGE:19685 same embryo
	EurExpress:euxassay_005148 same experiment
	MGI:4824614 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source