Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19704

Hirip3 HIRA interacting protein 3 ( MGI:2142364)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19704 EMAGE:19704 EMAGE:19704 EMAGE:19704 EMAGE:19704
"Pseudo-wholemount" of euxassay_006158. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006158_01 euxassay_006158_02 euxassay_006158_03 euxassay_006158_04
EMAGE:19704 EMAGE:19704 EMAGE:19704 EMAGE:19704 EMAGE:19704
euxassay_006158_05 euxassay_006158_06 euxassay_006158_07 euxassay_006158_08 euxassay_006158_09
EMAGE:19704 EMAGE:19704 EMAGE:19704 EMAGE:19704 EMAGE:19704
euxassay_006158_10 euxassay_006158_11 euxassay_006158_12 euxassay_006158_13 euxassay_006158_14
EMAGE:19704 EMAGE:19704 EMAGE:19704 EMAGE:19704 EMAGE:19704
euxassay_006158_15 euxassay_006158_16 euxassay_006158_17 euxassay_006158_18 euxassay_006158_19
EMAGE:19704 EMAGE:19704 EMAGE:19704 EMAGE:19704
euxassay_006158_20 euxassay_006158_21 euxassay_006158_22 euxassay_006158_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19704Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19704_wholemount_strong.wlz
19704_wholemount_moderate.wlz
19704_wholemount_weak.wlz
19704_wholemount_possible.wlz
19704_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19704_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8875
Entity Detected:Hirip3, HIRA interacting protein 3 ( MGI:2142364)
Sequence:sense strand is shown

>T8875
CTTCCAGTGGCTTCGCGGTGAGCTGAGGTCACAGAGGAGCTGGACACTGGGGGGGCACAATTATTGATTG
TAAACTGTTTATAAAGATTTGGAACCAAAAGAATATAACCAGAAATTCAGTATGTTAGCGAGAATACCGT
CTCCCTTCTGCCTTTCGAAACAAGCGACCTAATTTGTTAATCCCGACCACAGGGATTGATTGAACTGATT
ACGTTATAAAGACTGCCGGCGCCGAACCGGCTTCTAGCGAAAATTTTAACACGTAGTTTTAGCAAAGAGT
CGAAACCTCGCTCAAGACCCACCAGCTGCTCAGGAAAAAGCAAGGTCGGCCAAAGTTTTCCGTCTCAGTG
CTCCACATCGGAGGCTCAACGGTCCCATTCCTCACGAAGCCGCCGCACTGCGCGTGCGCAAACTACTGTC
GCTCGGGAAACCAGCCTTGAAGAAAAAAAGGCGGGAGCAACCAATCCCTGGTGACGCAAAATGGCGCCGG
GAGATGAGATGCGAAAATTCACTCGCAGCTTCTTTCGAGCTTGCCCGGACCTCAGCTCGGTTACGCACGC
CATCGTGCGACAGAAATTTTTGATTCACGTGGGTCGGGATCATCTTGAACCTGAAGAGAAGCAGGCGTTG
AAGCGGCTGGTGGAGGAGGAGCTGCCGAAAATGCAGGCGGATGCTGGTACCAGGGAAGGAAAGCCAGACT
TTATCAAAGTGAAGAGGTCTCCTGCTCCCTGCAGTGACCCAAAGAAGAAAAGGTTCCGCTTTAATTCAGA
GTCAGAATCCAGCTCTTCACCATCCAGTCCAGATGGCTCAGGACCCTCAACAAAGAACAGGACAACCAAG
AAGACATGTCTAAGAAGAGCCTTAAAGAAAGCAGTTGAGAGCACAGATGAAGACCACCAAACAGACCTGG
ATGCAAAGATGGGATTGGAGGAGAGCAGTGAGGGGGAAGCAGAGGGCTCTGTTAGATCAGGTAAGGTCAC
CGAGGAAGAGGAGGACATGAAACAAGAACAGAAGGGCCAGGTTAGAAAGCAAGCTGGGGCCAAAGACAAG
CAAGTACCACTCAAGGCAGACAGGAAGCAGGTTAGGGAAGAGAGTGGGAGCAGTGAGGAGGAGGCTGTGC
TGCAGAGAGCAAAGGTGGAGGGAAGCACAGGTGCTAACTGCCAGGAGGAGAGTGAAGAGAGCGGAGAGGA
GAGCCCAGCTAAGAAGAAAGAGCTTAGTGAGCCCAGATCCAGAAGTAACAGAGCAGAGAGAACAGCCAGG
GAGAGAAAGAGCTATAAGCAGAAAAGCAGGCCTGGGAGGCCAACTGGAGGCTTGCGAGACAGCGAGGCAG
AGAAGGAAGGGGGCACAGTGGGCAGTGGGGACAGCAGTGAGGAGGGGGAGGCAGAGAAGGAAGGAGGCAC
AGTGGGCAGTGGGGACAGCAGTGAGAAGGGGGAGGCAGAGAAGGAAGAGGGCACAGTGGGTAGTGGGGAC
AGCAGTGAGGAGGGAGAAGAGCACTCGGTGAAGAGAAAGAGCAAGGTCAGGACCCAGACCGAGAGTGGGA
GGAGGCAGAACACAAGCAGCAGGGATGACAGTAACAGCACACAGGAGCAAGCAGCTGCTCAAGGCACTAC
AAAGTCAGGCAGCCTAGGCAGCAGTAATGGTGACAGTGACACAGAGAGGGAAGTGAGTGACTCCCAGGCA
GGACAAAACACCAAAGAGGAGAGGAAGAGCCGCTCTTCCAACAAGAGCTCCAAGAACGGCCAGGCCCGAA
GTTGTTCATCTTCATCAGATTCGAGTCCAGAACCCACAGGTCAGAAGGGCAAGGCCCGAAGTTCCTCATC
TTCATCAGATTCAGGTCCAGAACCCACAGGTCGGAAGGCTGCCTCTCGCTGTGGAGAGGACCACCCAGCT
GTGGCTAGACTAAAGCGTTACATTCGAGCCTGTGGTGCCCACCGAAACTACAAGAAGCTGCTGGGCTCCT
GCAGCTCCCACAAGGCGCGCCTGAGTGTCCTCAGGGCTGAGCTGGAAGCACTGGGCATGAAGGGGAACCC
TTCCTTGGAGAAGTGTCGCGCTCTGAAGCTGCAGCGGGAAGAGGCAGCTGAAGTGGCTGCCCTGGATGTT
GCCAACATCATCAGCAGTACAGGCCGGCCACGAAGACGAAATGCCTGGAACCCTTCAGGGGAAGGGACCT
CCCCAGGAGAGACTTATCGCCGAACCCTGGACTCAGAAGAGGAGCAGCCGCGCCAAGCACCTCCAGACTG
GTCCCATATGCGTGATATCATCAGCAGTGACGGTGACAGCAGCTGAACTCCCGTGTCCATGGTGGACCTT
ACCCTGTTATACAAGAGCCTGGTCAGTGGAAGCAACTTCCAAAGACAGACTACAGTGGCAGGATCCAAGT
TATTGGCACACTACTCAATTTCATGTTCTCTAAGATGGCCTTGGGGTTCATGGTGGACCTTACCCTGTTA
TACAAGAGCCTGGTCAGTGGAAGCAACTTCCAAAGACAGACTACAGTGGCAGGATCCAAGTTATTGGCAC
ACTACTCAATTTCATGTTCTCTAAGATGGCCTTGGGGTTCATGTGCAAGCATCCTCTCCAGGCATTTGAG
TGGACTGTTGTTTGTTTTTAAAGACTCAATAAAAAATGTTTGTAATTACTTCAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6414655 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6414655 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19705 same embryo
 EMAGE:19702 same embryo
 EMAGE:19701 same embryo
 EMAGE:19703 same embryo
 EurExpress:euxassay_006158 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS