EMAGE mouse gene expression spatial database

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EMAGE:19710

2610021K21Rik RIKEN cDNA 2610021K21 gene ( MGI:1926017)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006155. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006155_01	euxassay_006155_02	euxassay_006155_03	euxassay_006155_04

euxassay_006155_05	euxassay_006155_06	euxassay_006155_07	euxassay_006155_08	euxassay_006155_09

euxassay_006155_10	euxassay_006155_11	euxassay_006155_12	euxassay_006155_13	euxassay_006155_14

euxassay_006155_15	euxassay_006155_16	euxassay_006155_17	euxassay_006155_18	euxassay_006155_19

euxassay_006155_20	euxassay_006155_21	euxassay_006155_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19710_wholemount_strong.wlz

19710_wholemount_moderate.wlz

19710_wholemount_weak.wlz

19710_wholemount_possible.wlz

19710_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19710_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8877
Entity Detected:	2610021K21Rik, RIKEN cDNA 2610021K21 gene ( MGI:1926017)
Sequence:	sense strand is shown >T8877 CTCGCTGCAGCGGAGGAGACGCCTGGCTTGGTCTCCGTGTCGGTGGCGATGTTCTTGTCAGAGGCAGGCG CAAGGCCCCGAGCAGGCGAAGCCAGCGCCTCAGAACGCAGGAAATGGGTGAAAGTATTTAAAGCATGCGA TGAAGACAACAAAGGCTACCTAAGCCGAGAGGACTTTAAGGTTGCGATTGTAATGCTGTTTGGGTACAAA CCCTCCAAGATAGAAGCGGATGCTGTGATGTCTTCAGTAAATCCCAACACTTCTGGTGTTTCACTTGAGG GGTTTTTGAGTGCCGTAAAGAGAAAGAAGGAAGCGCGACTCTATCGGAATGAGATAAGGCAGATCTTCAC AGCTTTCGACGTGCACTATCGTGGATTTTTAACTCTAGAAGATTTCAAGAGGGCGTTTAGTCGAGTGGCT CCCAAGCTACCAGCGAGGACAGTGCTGGAGGTGTTCAGGGAGGCAGATCAAGACTCAGATGGTCACGTCA GCTTCCGAGACTTTGAATACGCCATGAACCATGGGCAGTCAAAATGAGCCGGGTGCTTGTGAACTACTTG GACAGCTCGATGGATTGTAATCTAGTTGAAGGTCTCACAGACCTCAATCTAGGAAGGGTATGAAACTGTG TACTTGTGAGTAAACTCTTAGTTAGAGCTTTTGAATGCTGATATTGGACCTAGAGATGGGAATCTCCTGT GTGTGTCTCCAGCAGCCCTGAGTCAGGCTGCTACTGCTGGCCCTGCCACCCAGTGGGTTTGGAGTTTTTC CCCCTCTCTGCTCTTGTCAGCTTTCTGTCCTCTATCCTGTGGGTTGGAGGCTCTGCTAACTCCGCATGAG TCCCTCACTCTTGTCAGCTCCGGGAAAAGTTACCTTTGCTCTGCTAGCAGTGTCATTGCCATTCACTTGC TCCACCTCGGCTGAACATCAAGCTTACTGTGTCCAAGCAATGAGCTAGTGCTAGGAAAACAAAGACTCTG AGATGATGCGCCTGGGGTACAGAGAGGTGCACAGGGCACCTGTGTACAAAGCCTAGGAGGAAGCAGCTCT GGCTAGGCCATCAGCCATTCTTTATACAGAGAAGTCCAGGATGTAACTGGGGTCTTAATGGGCAGAAGGG TGTGGTCTTTGGTTCCCTTTAGGATAGCCTGGACCTGAGCCTGGGCTTCTTTCTTCAGAGAAGCTAGCTA GAGAAGCTGGCCGGCAGCCCTCTGCCACACACTTCTGGTTCTAGGTAGTTCTGCCCTGGACCTGAAGCTC CAGCCATCCTAGAGTTAGGAGTCTCCACTCCCTGCCATGCCCCTGGGCGCCAGTGATCACATCAAGTCAC AACATACAGCTCAGCATCTACTAAAGATAACTCTGAAATACCAACATTGTCCTTGTAAGTGTTTCACAAC ATTAACATTTGTGGTATAATTAATGCAATTTATGTTTTTAAAGTGTTAAACATTAAACGATCAAGTAGAA ATATAAATTTGTATAGCCAACATTCAACAAGCTTCATGCTCATATAAATTATTAGTAGATAGCCTTAAGA AAAATAAAGTTTTTAGCTATGCTAAACCAGAGGAACCTAAGAGATGGGATAACTAAATGTAATTTGACTG AAATGTAATTTGGTCTCCTAACGGGCCCCTAGGGACCATACGGTAGAGATGAACCAAGCCTGGACTTCCT CTGCTACTTGTGATAGTGTGGGAGACTGGGTGATGGATAGATAGGATTTCTACTGTTTTCATGATTTTTC TATAAATCTAAAATTATCCTCAAACGGAGTTTGTTAAAAATTAAAGGCTTAGTCAAACATTCATAAACTA AATGAAGAAGCAATGAAAATAATTTCTCAACATGAATGAAATATTATTTCAGAGGCAGAAATGAATTACT GGTATCATTGAGAAGTGTGTATGACTTTGTGCACAGTAGTCCAGTTTCTGACTTTGTTCAGCAAGTGTTT TCATTATAAGACAAACCAAATCATTGATCCTGATCTCAAAATATTAAAGAGATATTTTTGATTTCTTAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6414718 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6414718 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19709 same embryo
	EMAGE:19706 same embryo
	EMAGE:19707 same embryo
	EMAGE:19708 same embryo
	EurExpress:euxassay_006155 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source