EMAGE mouse gene expression spatial database

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EMAGE:19717

Szt2 seizure threshold 2 ( MGI:3033336)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006209. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006209_01	euxassay_006209_02	euxassay_006209_03	euxassay_006209_04

euxassay_006209_05	euxassay_006209_06	euxassay_006209_07	euxassay_006209_08	euxassay_006209_09

euxassay_006209_10	euxassay_006209_11	euxassay_006209_12	euxassay_006209_13	euxassay_006209_14

euxassay_006209_15	euxassay_006209_16	euxassay_006209_17	euxassay_006209_18	euxassay_006209_19

euxassay_006209_20	euxassay_006209_21	euxassay_006209_22	euxassay_006209_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19717_wholemount_strong.wlz

19717_wholemount_moderate.wlz

19717_wholemount_weak.wlz

19717_wholemount_possible.wlz

19717_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19717_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8900
Entity Detected:	Szt2, seizure threshold 2 ( MGI:3033336)
Sequence:	sense strand is shown >T8900 GCCGAGCTCTGATCCGCCTTGTCCAGTGGCAGAATGCGCGAGCCCACCTCATCTCCTGCCTGCTCAGCCA GAAGCTCGGTCTCTTCCACCACTGTGGCCAGTTAGACTTTCCCATGCGTGATGGAAAGGAGCCAAACCCA TTCCTGCTGCCGACCATGGAAGTGGAGACCCTCATCCGGAATGCAAGCCCTCCACTGAGTCGCGAACAGG GCCGGCTGAGTGGCTCCTCTCGTGGGGGAGGTCCCCTTTCCTTGGACACATTTCCCTTTGATGAAGCTCT GAGGGACATCACAGCTGCTCGCCCGAGCTCTACAGGTGGTCCTGCGCCCAGACCTCCAGATCCTGTGACC TACCACGGGCAGCAGTTCTTAGAAATTAAGATGACAGAACGAAAAGAGCTGGAGCGCCAGATGAAGATGG AGAACCTGTTTGTAACCTGGCAGCAGCGTTCTGCACCAGCGAGCATGCCCATCAGTGCTGGGGAGCTGGA GACCCTGAAGCAGTCATCCCGCTTGGTGCATTACTGCGCGACTGCTCTGCTCTTTGACCCAGCTGCCTGG CTGCACGGACCCCCAGAGACTTGTGCACCCTCTGAAGGGCAGCGACGACCGTGCCCTGAGTCAGGGTCTG GAAGCCGAGAGGTTCCCACAAGCTGTGAATCCTTGGATGTGCCTCCTCCAGGAGCCCGAGAGGAGCCATG GCTGAAGGAGCTGAGCTTGGCCTTCCTGCAGCAATACGTGCAGTATCTGCAGAGCATTGGCTTTGTGCTG GTGCCACTCCGGCCCCCCTCACCTGCCCGCAGTACCAGCCGGCTACGGGCTATGGCTATCCTTGGAACAG AGGGTCGAGGCTCCTTCTCCTGCCCCAAAGCCAAGGCTGAGGGGAGCCCAAAGAGCACCAGTACTCCGGT CACCACCTACCACCTGCAGCGGGCACTGCCTGGGGGCATCATCCTCATGGAGCTGACCTTCCAGGGCTGT TACTTCTGTGTCAAACAGTTTGCTCTGGAATGTTCCCGAATCCCAATGGGACAAGCCGTCAACTCTCAGC TGTCCCTGCTGTTCACAGAGGAATGTGACAAGGTCCGTGATCTGATGCATGTGCACTCCTTCAGCTATGA CTTCCACCTGCGCCTCGTGCACCAGCACGTGCTCGGTGCCCACCTGGCACTGCGGCACGGCTACCACCTC ACCACCTTCCTGCAGCACTTCCTGGCCCACCACCCTGATGGACCCCACTTTGGCCGTAATCACATTTACC AAGGGACGCTGGAGCTCCCCACCCCACTCATTGCTGCCCACCAGCTGTACAACTATGTGGCTGACCATGC CAGTTCTTACCACATGAAACCACTGCGAATGGCCCGGCCCGGTGGTCCGGAACATAATGAATATGCCCTG GTGTCAGCATGGCATAGCTCTGGCTCCTATCTGGACTCGGAAGGGCTTCGGCATCAGGATGACTTTGATG TGTCTCTGCTTGTCTGTCACTCTGCTGCACCCTTCGAGGAGCAAGGAGAGGCTGAGCGACATGTGCTGAG GCTTCAGTTCTTTGTGGTGCTAACCAGCCAACGAGAACTTTTCCCAAGACTCACTGCTGACATGCGCAGA TTCCGGAAACCCTCCAGGCTTCCCCTTGAGCCAGAGACTCCTGGGAGCTTGGTTGGTAGCCCCAGGGAAG CCTCAGGGATGATGCTGGCCCCAGGGCCAGCTCCTCTGTTCCCACCATTGGCTGCAGAAGTGGGTATGGC CCGGGCACGGCTAGCTCAGCTGGTTAGGTTGGCTGGTGGGCACTGCCGGCGGGATACCCTCTGGAAGCGC CTATTCTTGCTGGAGCCACCAGGGCCTGACCGGCTAAGGCTTGGAGGGCGCCTGGCCCTGGCAGAGCTGG AGGAGCTCTTAGAGGCAGTCCACGCCAAGTCCATTGCGGACATTGACCCCCAGCTGGACTGTTTCCTCTC CATGACGGTCTCCTGGTACCAGAGCCTCATCAAGGTTCTCCTGAGCCGATTCCCCCAGAGCTGCCGCCAT TTTCAGAGCCCAGACTTGGGAACCCAGTACCTGGTTGTGCTGAATCAGAAGTTCACCGACTGCTTTGTCT TGGTGTTTCTGGACTCACACCTGGGAAAGACGTCTCTGACGGTGGTTTTCCGAGAGCCCTTCCCGGTGCA GCCCCAGGACAGTGAGAGCCCTCCTGCCCAGCTGGTCTCTACCTACCACCACTTGGAGTCTGTCATCAAC ACAGCCTGTTTCACCCTCTGGACCCGCCTTCTCTGAGAAGTGGACCAGTGACTCTGTTGATTGAATCATG GATCTATAAGCTTGCCCGCTTGAGGCAGGACAGACTGGCACTTAGGAACCCCAGGGTAAACTCAGACACA GAATGACACTAAAGGACTGAGTATGTCTGGGAGAAGTGCTGCTACAGCCTGGCAACAGGAACATAGCTGC TGAACAGCCCGGACTCGCCTCAGGCCCCTGGGCCTGTGCAGAAGAGGATGCCTCCTTCCTGCACACCTGG TGGAGTGCCAGGGAATGTCACATGATCTGACAAGCTCTCACCTCTAAGAGCTCTGCCACTTGGCCATCAC TGTTGGACATTACCATTGAGATAGACTTGGTTCCGATCTTGACTCGGCCTGTCAAGCACCTGTGACCAGT CTTACCTCCTCCCGTCCCCACTGGACCTTTGGTTCCTCATCTGTGGATAAGGAGGGCTCAGAAGTCTCCT GTGTCCACAGCAACCCTGGAGGATTTGAGCTTGAGCTGTGTGTCCCTTCTCAGTGGGAGGGAATTAACCT GGAGACATTGAAGCATTAGACACTATGTGTCCCCCACCCCTGTAATATACACATATGAGAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6415770 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6415770 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19718 same embryo
	EMAGE:19721 same embryo
	EMAGE:19720 same embryo
	EMAGE:19719 same embryo
	EMAGE:19722 same embryo
	EurExpress:euxassay_006209 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source