EMAGE mouse gene expression spatial database

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EMAGE:19727

Calcoco1 calcium binding and coiled coil domain 1 ( MGI:1914738)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006214. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006214_01	euxassay_006214_02	euxassay_006214_03	euxassay_006214_04

euxassay_006214_05	euxassay_006214_06	euxassay_006214_07	euxassay_006214_08	euxassay_006214_09

euxassay_006214_10	euxassay_006214_11	euxassay_006214_12	euxassay_006214_13	euxassay_006214_14

euxassay_006214_15	euxassay_006214_16	euxassay_006214_17	euxassay_006214_18	euxassay_006214_19

euxassay_006214_20	euxassay_006214_21	euxassay_006214_22	euxassay_006214_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19727_wholemount_strong.wlz

19727_wholemount_moderate.wlz

19727_wholemount_weak.wlz

19727_wholemount_possible.wlz

19727_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19727_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 weak expression: see section 09 10 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T533
Entity Detected:	Calcoco1, calcium binding and coiled coil domain 1 ( MGI:1914738)
Sequence:	sense strand is shown >T533 TCCTCGAGNCTGTTGGCCTACTGGGTGCTGTTTTTGTTGTTGGTGAAAGGTGAGGGGAACAGCTGATCCG TCCGTGGGGACATCAGCCATCTTGAGGACAGCATGGAAGAGTCATCACTAAGCCGGGCACCATCCCGGGG TGGAGTCAACTTCCTGAATGTGGCCCGCACCTACATCCCCAACACCAAGGTGGAATGTCACTACACTTTG CCTCCGGGTACCATGCCCAGTGCCAGTGACTGGATTGGCATTTTTAAGGTGGAAGCTGCCTGCGTTCGAG ATTATCACACGTTCGTGTGGTCCTCAGTGCCAGAAAGTACAACTGATGGCTCCCCCACCCATGCCAGTGT CCAATTCCAAGCCAGCTACCTGCCCAAACCAGGAGCCCAACTCTACCAGTTCCGGTATGTGAACCGCCAG GGCCGGGTGTGTGGGCAGAGCCCCCCCTTCCAGTTCCGAGAGCCAAGGCCCATGGATGAACTGGTGACCC TGGAGGAGGCTGATGGAGACTCTGACATCCTGTTGGTTGTCCCTAAGGCCACCG
Notes:	The probe template was PCR amplified from IMAGE:1498618 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1498618 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19725 same embryo
	EMAGE:19728 same embryo
	EMAGE:19724 same embryo
	EMAGE:19726 same embryo
	EMAGE:19723 same embryo
	EurExpress:euxassay_006214 same experiment
	MGI:4823595 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source