EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19765

Mpp2 membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) ( MGI:1858257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006254. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006254_01	euxassay_006254_02	euxassay_006254_03	euxassay_006254_04

euxassay_006254_05	euxassay_006254_06	euxassay_006254_07	euxassay_006254_08	euxassay_006254_09

euxassay_006254_10	euxassay_006254_11	euxassay_006254_12	euxassay_006254_13	euxassay_006254_14

euxassay_006254_15	euxassay_006254_16	euxassay_006254_17	euxassay_006254_18	euxassay_006254_19

euxassay_006254_20	euxassay_006254_21	euxassay_006254_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19765_wholemount_strong.wlz

19765_wholemount_moderate.wlz

19765_wholemount_weak.wlz

19765_wholemount_possible.wlz

19765_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19765_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8558
Entity Detected:	Mpp2, membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) ( MGI:1858257)
Sequence:	sense strand is shown >T8558 GCCGCTTTAAGAGCGCGGCTAGCGATTGACAAGCAATAACCGCGGAGCGCCGGGCTGCGCGGAATCGCCC CAAGCTAGGGGCTCCCCAAAACGCAGGACCATCAGAATCCACACTGCCTCTATTCACCATGCCGGTTGCT GCCACGAACTCCGAGTCTGCTATGCAGCAAGTCTTGGACAACCTGGGATCCCTGCCCAATGCCACTGGGG CCGCGGAACTGGACCTGATCTTTCTTCGAGGCATAATGGAAAGTCCGATAGTCAGATCCCTGGCCAAGGC CCACGAGCGGCTGGAGGAGACGAAGTTGGAGGCCGTCCGGGATAACAACCTGGAGCTGGTGCAGGAAATC CTTCGGGACCTGGCAGAGCTGGCAGAGCAGAGCAGCACCGCTGCAGAGCTGGCACGCATCCTCCAGGAGC CCCACTTCCAGTCCCTCCTGGAGACACATGACTCAGTGGCTTCAAAGACCTATGAGACACCACCCCCAAG CCCAGGCCTGGATCCCACGTTTAGCAACCAGCCAGTACCTCCTGATGCGGTGCGCATGGTGGGCATTCGG AAGACTGCAGGAGAACACCTGGGTGTGACATTCCGAGTGGAGGGTGGTGAGCTGGTGATTGCCCGCATTC TGCATGGGGGCATGGTGGCTCAGCAAGGTCTGCTGCATGTCGGTGACATCATCAAGGAAGTGAATGGACA GCCAGTGGGCAGCGATCCCCGGGCACTGCAGGAGCTCCTGCGCAGTGCCAGTGGCAGTGTCATCCTCAAG ATCTTGCCCAGCTACCAGGAACCCCACCTGCCCCGACAGGTATTTGTTAAATGCCACTTCGACTATGACC CTGCCCGGGACAGTCTCAGCCCCTGCAAGGAGGCAGGCCTGCGCTTCAACGCGGGGGACTTGCTCCAGAT TGTAAACCAAGATGATGCCAACTGGTGGCAGGCATGCCACGTAGAAGGCGGCAGTGCCGGCCTCATCCCC AGCCAGCTGTTGGAGGAGAAACGGAAAGCATTTGTCAAGCGGGACCTGGAACTGACCCCGACCTCAGGGA CCCTATGCGGCAGCCTTTCAGGAAAGAAAAAGAAGCGAATGATGTATTTGACCACCAAGAATGCAGAGTT TGACCGCCATGAGCTGCTCATTTATGAGGAGGTGGCCCGCATGCCCCCATTCCGCAGGAAAACCCTGGTA CTGATTGGCGCGCAGGGTGTGGGCCGGCGCAGCCTGAAAAACAAGCTCATTTTGTGGGATCCAGACCGCT ACGGCACCACGGTGCCCTACACATCACGGAGGCCCAAGGACTCAGAACGGGAGGGGCAGGGCTACAGCTT TGTGTCCCGTGGGGAGATGGAGGCTGACATCCGAGCCGGGCGCTACCTAGAACATGGCGAGTATGAGGGC AACCTGTATGGCACACGGATCGACTCCATCCGGGGCGTTGTTGCCTCTGGCAAGGTGTGCGTGCTGGATG TCAACCCGCAGGCGGTGAAGGTCCTGAGGACAGCTGAGTTTGTCCCTTACGTGGTGTTCATAGAGGCTCC TGATTATGAGACTCTACGGGCCATGAACCGGGCTGCGCTGGAGAGTGGCGTGTCCACTAAGCAGTTGACG GAGGCAGATCTGAGGCGGACAGTGGAGGAGAGCAGCCGCATCCAGAGGGGCTATGGGCATTACTTCGACC TCAGCCTGGTCAACAGCAACCTGGAGAGGACCTTCCGTGAGCTCCAGACTGCCATGGAGAAACTTCGCAC GGAGCCCCAGTGGGTGCCTGTGAGCTGGGTGTACTGAGGCTTGCTCTGGACCTTGTTACTGCCTGGTTGG GAATCCAACTTCCACTCCACCTGCGATGCTCCCCCATCCTGCTAATGGTCTTCGTCCCCATCTCTGGTTT TCCCTGGACTGCAGTTCCCAGCAAGGGCTGGCTTCAGTATAGGGGTGTGCACTGCCAGGGAGGTAGGTGT TCATGGGGTACCTCTGTACCCAGGTGCTGCCCACTCCCCGTGTCTACCGGCCACCAGACACCCTTTTCTA AGGATCATGCTGTGTCTAGGAGTGTCTCAGATCACTCCTGTAATTCCCAGTCCAGGGAAGAGCAGAGCTG CTTGGGACCTTGTGGTCAGGAGGTTCGTGACACTGCCAGCCTTTTCCTCCTCTGGACTGGCCACAACCAA CCTGTTCCCTGGCCCCTCCCACCCCTTACCATAGTCCTAGGAACAGCCTTGGGCTGTTCTCATATGGCCA GGTACTCAGACCACTGGTAGCTAGGCAGACCCAGGTAATAGTGCCAGCCTGGGCCATGTTAGAGGCTGGG GTCACCCCGTCATGAGCCAGTTGCTGCAGCTGGCGATGCAGGTCTCAGCTCCGTGAGGAGCAAACATAGT CTGTTCCCTCCCTCACTCCAAGCTTCTCACTGCCAAAGTCTCTCCACAGACTTTCTCAATGTGTCTCTCT TGTAAGCCCTGCTCCTCAGACTTGCGGCTGGTGGGTAGCAGTAGGACTCAGCCCAGGTTGACGATAGGGC CTAGAGAGCTGAACCCTCTATTGTTGGGACCCAAAGTACCCCCAGGCTCCACAGCCCGCAGCAGGTTTTA TTCACTTCAGGCTTTTCTCTGGATGGATCTGGGTGAACCGCTGGCATTAGGGCTTATGAGGGGCGGGGAG ACTGTGAGTGGGGGCTTGGAAACAGATTAGCTTTAGTGGCACTTGCCACACAGCTCCAGGGGGTACCATT GGCACTGCAGGCTGGGCAGTCTGTGGCACTGCCTGCTGTGGTGCTGGTCTGTGTTTCTGGCCTTGGTGTG TGTGTGTGTGTGTGTGTGTCGCACATGCACACATTTGGTTGAGTTTGAGGGGGGTCAGAGGATTGCCTTC TCTTGGAGGTGGCTCTTTGAGGTTCCCTTATGGGTTCCCCTCAACTCCACCATTTCTTACAACCCCCCAT TCCAGACTCCAGAGCCTGAATCTTCCTCTACTCTCCCATCTTCCCTTGTCCTCAGTACCAGCCTTTAGCC CTTGGGGAGGGTGATTTTTGTAAGATAGGATAATCCTTCTAAGAACCCCATCCATGACATCTGGAGACAT TTCATTCCTCATTCTTGAGGGGCCCCATGTCCTCACAGGAGACCCCCCTTGGCACCTCCATCCTGTGCCT CCCAGTTCTCCCAAGCGTCAATCTTTGCTGCACTGTCAGCCCAAGCTTGTCCCTGAACCACTGTGTGCCC ATATCCTAGAGAAGGGGAGGGAGGGAGAATAGACAGAATATTTATTACAAATGCTAGAAATATATTTATT ATATTAGGAATCTCATTTGCATTGGCATCGGATGTGCAGACGAGACTAAAGGTGGGACCCCCCGCCCGCT GCTCATTTGCATAGCTCTGCTAGCACCTACCTGTGGTTTATCCTTTAGTCAATCAATGTCCTTTCCTTCA ATATCTTGCTGTGACCCCTGACCCTTACTTAGCCAAAGGGGACTGCCCTCCTTGCCAGTTCCTTCCTCCC CTGGAGGATGAGCGCAGGCTCAGAAAAGCATCTGTGACAGCCCCCGACCCCCCTTGATTAACCGACAGAG TTCCCCAACCAAAAGACCACGGCCTCTGATAGAGATCTGGTGGGCGCCATGCTGGAGAGTTGGTACTAGT TCCTCCCTGGATTTATACTGGAGGTATTTATCCAGTGTGGTATCAACCCCCACATCCTCCTCTCTAGTAA TGTACAAGGGCCCCTCCTCTCTGAACAGCCTCCTACCTCTCTCTCCCACTGAGCCTGGCTCTACAACCCG TCCATGCACTGGCCAGGGGGGAACAGAGGCTGTGAGCCTCCTCAGCTCACCTTGCACGTGTTAGAACATG TTCGTGCCCACCAAGCTAGCTCACTCTGTGACTCTGTCGTCTCTCTCTCCCTCCCACCGTGGCCTCTTGT ATTTGGGGACCTCAGGAGGAGGAGGAGGAGGAGGACCCTGCCGCCCCTGCCATGGTCCACACTCAATCCC AGCCCGTTGCTGTCCCTCTGTCTCCCCCACTCCCCACCCCCAGCCATCTAGTTTTTAAGTTTTCTTCTTT TCCTTTGATTAAACGTGACAGCAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5709543 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5709543 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19763 same embryo
	EMAGE:19762 same embryo
	EMAGE:19761 same embryo
	EMAGE:19760 same embryo
	EMAGE:19764 same embryo
	EurExpress:euxassay_006254 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source