EMAGE mouse gene expression spatial database

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EMAGE:19785

Tchp trichoplein, keratin filament binding ( MGI:1925082)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006285. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006285_01	euxassay_006285_02	euxassay_006285_03	euxassay_006285_04

euxassay_006285_05	euxassay_006285_06	euxassay_006285_07	euxassay_006285_08	euxassay_006285_09

euxassay_006285_10	euxassay_006285_11	euxassay_006285_12	euxassay_006285_13	euxassay_006285_14

euxassay_006285_15	euxassay_006285_16	euxassay_006285_17	euxassay_006285_18	euxassay_006285_19

euxassay_006285_20	euxassay_006285_21	euxassay_006285_22	euxassay_006285_23	euxassay_006285_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19785_wholemount_strong.wlz

19785_wholemount_moderate.wlz

19785_wholemount_weak.wlz

19785_wholemount_possible.wlz

19785_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19785_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 17 18 19 20 weak expression: see section 10 11 12 13 14 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8482
Entity Detected:	Tchp, trichoplein, keratin filament binding ( MGI:1925082)
Sequence:	sense strand is shown >T8482 GGACCCTCGGAGCGAGCGGGCGCGTGATCGAGCTAAGGCCGGCGACCCTTCTCCGCGAACTTCGGACACG GAGTGCATGAGTCAGAGATGGCTCTCCCCACGTTGCCCTCTTATTGGTCCAGCCGGAAACACATGGATCT TCGCCAGCGCCAGCATCAGCAGGAGGACCAGTTTCGCCAGCAGTGGGAACAGAACAGTCGCTATTTCAGG ACCTGGGACATCCACAACTCGAAACAGATCGAATGGAGCTCCAAGATCTCCTACCAGCGGAGCATGCACG CCTACCATTGTGAGAAGATGAAGGAGGAGAAGAGGAAGATTCTGGAGCTCCGGCGGGAGAGGCTCAGAGA GCTTCTCCTGGAGGAACAGGACTTGCTGGCTGCGGAGCTGGATGAGCTCAGGCTGAGCATGGGCCTGCGG GAGCAAAGGCTCCGCGAGCAGCACCAGGACCTGAAGTCAGCCCGAGAAGAACAGAGGAAGCTGATTGCTG AACGACTTTTATATGAACACTGGAAGAAGAACAACCCCAAACTTCGAGAGCTTGAGTTGGACCTTCACAA GAAGCACGTGATAAATTCTTGGGCAACACAGAAAGAAGAAAAAAAACAGCAAGAAGCCACAGAGAAGCAG GAGAACAAGCGGTTGGAGAACCAGTACGCAGCAGCCCGCCGGGAAGCCGAGGCGAGGATGAGAGTCGAGG AGGAGAGGAGGCAGCTAGAGGGGCGCCTGCAGGCCGAGGCGCTGCGCCAGCAGATGGAGGAGCTGAAGCA GAAGGAGATGGAGGCAACCAAACTGAAGAAGGAACAGGAGAACCTGCTGAGGCAGCGCTGGGAGCTGGAA AGGCTGGAGGAGGAAAGGAGGCAGATGGCCGCCCTCCGGCGCAAGACAGAGCTGGGGCGCTTCTTGAAAC ATCAGTACAACGCGCAGCTCAACAGGCGCACCCAGGAGATCCAGGAGGAGCTGGAGGTAGATGGGCGGAT CCTGCAGGCCCTCCTGGAGAAGGAAGGCGAGTTGCAGCAGGTGGAACTGGCCAGGCGGGAGCAAGCCCGG GCCGATGCGGCCTGGATGAAGCAGGTCATCGAGGAGCAACTGCAGCTGGAGAAAGCACGGGAGGCAGAGC TGCAGCAGCTGCTGAGGGAGGAGGCCAAGGAGATGTGGGAGAAGAGAGAGGCCGAGTGGGCCCGAGAGCA GGTCGCGCGGGACAGACTGATGAGTGAGGTTCTGACCGGGAGGCAGCAGCAGATCCTGGAGAAGATTGAA CAGAACCGGCGGGCACAGGAGGAGACCCTCAAACACAGGGAGAAACTCATCAGGAGTCTCGAGGAGGGGA AGCAGTTAGCTCAGCGAGCCAAGGAGGAGAGTGAAGAGCTGAAGTTGGCCAGGAAGCAGGAGCTGGAAGC CCAGGTGGGGCCCAAGGGCAAGGGCGGCCTCGCAGGACACTACAGAACAGAGCTCGGCCGAGGTCCTTGA GTCCAGTCCATTGCTTGCTTACATCCAAATCTCTCTCTGCTGCAGGCTCCATCCTGGTTTCCTAGTCTGT GGAAACCAGGCTAATGTCACTGTCCCCTTTTCTTCTTTCTATCCTGTTCTCCAAACACATGGCTGCATTC AGAGCTCTCACATGGTTCACAGCACCATCTGTATGGCCGTTCCCAGTCCTCAGGTTCTTGTCCTGGTTTC TGGCACTTGCCATCTGTGTCCTTGCTTGAAGGAGGGCTGTGCCCTCTGGGCCTTGTTTCTGGTGACTCTG TTCTCGGTGTCCAGGTTGCAGAGCGCCAGGGCCAGGAATGGGAAGCAGCCCGGCAGGAGGAGGAGGAAGA GGAGGAGGCCAGGCAGGCGGAAGAGCACTCCAATGCTTTGCTGCAGCAGGAGGCGAAGACCATGGCAGAG AAGGGCTATCAGCCCAAGCTTCACGGACACCTCAGAATTGCTTGGGACTGACGTTGAGCACAGAGAAGCA AACCGACTCACTACCGCTCCAGACAGTTGTACGGTGTTGGTGCTGGCCAGTGCTCAGGGTGCTGCTAGCT AGTTGAGCCATACCTGTCAGGCACATCATGGGCCATAGATGTCAGGATGCCATGGGTGTGCAGCGTCCCC TGAGAGCCCTTCTCACCCCGACAGGCCTTGGCTTCAGTGCCACCAGAGATCAGGAATGCTTTATAAGTAC ATGTTAGAGTTAGTCTAAGAAGAAGTCATGTTCCCTGACAGACAGGAGTACCATGACAGGAATACGTAGG TTCCTCTCATCTCACCTGTAGCTGTCAGCTCAGGGAGGACCAGCCACAGAGAGGAGGGAGCTGTTTAGGT TTTCAAATTGCTGTTCAGAAGTTATTCTATTGAGGGCTCATAGGTCTTGGTGCAGTTATTGCCAGTTATG AGCTGTGTAGCCCCAAGGCCCATGTGTGTACTGTGGAAGAGCTGGCAGTCTGGAGAGCCGGTGACAAAGG CGCCCTTGTTTGGAAGTGTTGTGAGGGCTACCCCTAGGGGTTGGGGGTTCTCAGCGTGGAGTACTTAACC TTCCACTTGTGTGGTCCTCTGCCCAGCTCCACACCACCAAGAAGAAAAGAATCCTACAGAGCACTTGAGC CCAACATGTGCCACCCCGAAAGAGTCCTCAAGGTCACTTGGTTGGCTAGCCGACAAAGGCAGAGGCTTGA CTAGGGACCTCCTGTGTCTGTGGAAGCCCAGTGTGCAGCCCCATGGGAATTAAGTGCCCAGAGAGACATG TGACCCTGGAGAAATGGCATGAGACTCGGAATCACAGATGAGCTTGTCCCCACTGTTAGATCCCAATACA AGTGGCCATGGGTCCTCATGGCGACAGTCGGGTTGTTATGGCTTGTATCTTTCCGTAGCCTTCCTCGAGT TGGCTTTCACCCCTATATTCCAGATTCTGCCCACTCTAGCTGAATATACGGTGTTGTTCCTGGTTATGGG AACCGAGAGTTTGCCTGGGCTCTGAGGGCTGTATCACTTCCCTTCCATCACGGAACCGGTTCTCAGCCTG TTTCATTTCCTGCTGAGGTCCTTGGATGTGGCCCCCAGCTCTGTTTCCACTACACATTTGTGAGCACTCG CCCTCGTCAAATGCTCACTAACTGAAGTAAAGCTCTATCTCAGTATCAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:5704036 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5704036 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19786 same embryo
	EMAGE:19784 same embryo
	EMAGE:19788 same embryo
	EMAGE:19789 same embryo
	EMAGE:19787 same embryo
	EurExpress:euxassay_006285 same experiment
	MGI:4828642 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source