EMAGE mouse gene expression spatial database

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EMAGE:19794

2410001C21Rik RIKEN cDNA 2410001C21 gene ( MGI:1913654)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006308. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006308_01	euxassay_006308_02	euxassay_006308_03	euxassay_006308_04

euxassay_006308_05	euxassay_006308_06	euxassay_006308_07	euxassay_006308_08	euxassay_006308_09

euxassay_006308_10	euxassay_006308_11	euxassay_006308_12	euxassay_006308_13	euxassay_006308_14

euxassay_006308_15	euxassay_006308_16	euxassay_006308_17	euxassay_006308_18	euxassay_006308_19

euxassay_006308_20	euxassay_006308_21	euxassay_006308_22	euxassay_006308_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19794_wholemount_strong.wlz

19794_wholemount_moderate.wlz

19794_wholemount_weak.wlz

19794_wholemount_possible.wlz

19794_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19794_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8449
Entity Detected:	2410001C21Rik, RIKEN cDNA 2410001C21 gene ( MGI:1913654)
Sequence:	sense strand is shown >T8449 GTTTCGCCAGAGACCCCGGAAGCGACGGCTTCTCGTCTTTGCTAAGGCTTCTGGTTTTTGCTACGGTTTC TGATTTCGGCTTTGGGATGGGTTGCGACGGAGGCACCATCCCCAAGAGGCACGAGCTGGTGAAGGGGCCG AAGAAGGTCGAGAAGGTTGACAAAGACGCTGAACTGGTAGCCCAATGGAACTACTGTACCCTAAGTCAGG AAATCCTGAGGCGCCCAATAGTTGCCTGTGAGCTTGGCAGACTGTATAACAAAGACGCGGTCATCGAGTT TCTGTTGGACAAATCTGCTGAGAAGGCCCTGGGGAAGGCGGCATCGCACATCAGAAGCATCAAGAATGTG ACAGAGCTCAGGCTTTCTGACAACCCCGCCTGGGAAGGAGATAAAGGGAACACCAAGGGCGACAAGCATG ATGACCTCCAGAGAGCGCGGTTCATCTGCCCTGTGGTGGGCCTGGAGATGAATGGCCGGCACAGGTTTTG CTTCCTCCGATGCTGCGGTTGTGTGTTTTCTGAACGCGCCTTGAAGGAGATAAAAGCTGAAGTGTGTCAC ACGTGTGGGGCTGCCTTCCAGGAGGAGGACATCATTGTGCTCAATGGCACCAAGGAGGATGTGGAGATGC TGAAGAAGAGGATGGAGGAGAGGAGGCTGAGGGCCAAGCTGGAAAAGAAAACAAAGAAACCGAAGACAGC GACCGAGTGTGCGTCAAAGCCGGGTACCACACAAGATTCCGCAGGGCCATCAAAAGTGAAGTCTGGCAAG CCTGAGGAGGCGGACCCTGATCCTAGAGAGAAGAAAAGCACCCCGGCTCCCAGGGGCGCAGCAACTAATG GGAGTGCGTCTGGGAAAGTTGGCAAGCCCCCATGTGGGGCCCTGAAGAGGTCCATTGCAGACAGCGAGGA GTCTGAAACCTACAAGTCTATCTTCACCAGCCACAGCTCTGCCAAGCGCTCCAAGGAGGAGTCTGCGCAC TGGGTCACCCACACATCCTACTGCTTCTGAAGCGGGACCGGGCCTTCACCCTGGGTTTCCCGTGGCCTTC TGTGCCACACTTGCTTGGTGTGGCACTGTGCTATAAAGCTGTCCTCTGCAGTCCTCAGCCAGCGTGGTCA CTTGCTGTGAGATCTTGAGGGCCCTGGGAGTGAGAAGTGGTGTGTGACCTCCCTAGTAGCAGGTCCACAG GCCGTACCTCTCACCTAGCCTGGTGACTGCTGCTTCTATTAGGTAACAAAAGACTCCAGCTCTCGGTGTA CACTCAGCTCATAATGGTCAAGGAGGGAGTGGCCCCGAGCTTAGGGACAGACTTCCAACAGTCTTAGACC TTGCTCCTCACAGGGTTCTTCTTGCCTGATCAAGGACTGCAGAGAGGACGCATGCCTGACTGCCTGGTGC ACACTAACATCCTTTCTCTGTCTTCCTTCTCTCTCCCTCCGCTATCTTGGTAAGAGAATGGCAGCCATCC AGGTGCCGGTGGCAGGCAGGAATGACTCCTTTTCCATCCCCACAGTTAAAATTGGGACCTCTGTGACACT TCATGTGGTGTGATAGATCTGCCTCTGGGCTGGGCAGATGGCTCACTCAGTGGATAAAGCACTCGCCATG GACACTTGTGGACCAGAGTTTAGATCCCCAGAACCTTTATCAAAGCCCGGTGAGTGCAGTAGCTGCCTGT GATCCCAGCCCCTGTAAGGCAGGGTTCCCTCAGCAAGGCACTGAGTTCAGGGTTCGAGGAGAACCTGCCT CAGACACTAGCTGGAGTGCAGTTGAGAGATTCCCAGTGTCAGCCTCTGGCCTCCACACTTAAAGTCCCTG TTCCCAAACCAAACATGGTGGCATGTCTAATCCAAGCAGTCAGAAGACTGAGGCAGGAGGATCGCCGAGA TGTCAGAGCTGGCAAGGTTAAATAGCAAACCCTCACCTCAGGAAACAACCCCAGAAAAGCCCCTGTTCCT GCTGCCCTCTGGTTTGCTGCTTGCAGGCGATGAGGCTTGGCCTCAAATACACTTGCATTTATTCTGGGAC AGTGCTGGGAGCATGGTAGGAGTGTGCAGCAGGGTGGGAGTGAGCATGGTCCAAGGGACCCTGGGCGCAT GACCTTTGTACTTAGAAAATTGAAATAAAGGTTGTAGAAATAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5701585 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5701585 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19792 same embryo
	EMAGE:19793 same embryo
	EurExpress:euxassay_006308 same experiment
	MGI:4822671 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source