EMAGE mouse gene expression spatial database

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EMAGE:19813

Gpc2 glypican 2 (cerebroglycan) ( MGI:1919201)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006296. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006296_01	euxassay_006296_02	euxassay_006296_03	euxassay_006296_04

euxassay_006296_05	euxassay_006296_06	euxassay_006296_07	euxassay_006296_08	euxassay_006296_09

euxassay_006296_10	euxassay_006296_11	euxassay_006296_12	euxassay_006296_13	euxassay_006296_14

euxassay_006296_15	euxassay_006296_16	euxassay_006296_17	euxassay_006296_18	euxassay_006296_19

euxassay_006296_20	euxassay_006296_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19813_wholemount_strong.wlz

19813_wholemount_moderate.wlz

19813_wholemount_weak.wlz

19813_wholemount_possible.wlz

19813_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19813_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 06 16 17 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 15

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 15 16 17 18 19

vagus x ganglion

strong

regional

strong expression: see section 06 14

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 14 15 16

trigeminal v nerve

strong

regional

strong expression: see section 09 15

spinal cord

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 12

cervical ganglion

moderate

regional

moderate expression: see section 07 14

thoracic ganglion

moderate

regional

moderate expression: see section 08 09 10 11

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 12 moderate expression: see section 04 08 09 10 13 14

not examined

regional

not examined expression: see section 02 03 04 20 21

neural retina

strong

regional

strong expression: see section 02 03 04 20 21

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8526
Entity Detected:	Gpc2, glypican 2 (cerebroglycan) ( MGI:1919201)
Sequence:	sense strand is shown >T8526 AGTTTTAGGGGACACGCTCCTATTCTTTTCTTCGAGACGTCCCGCGGCCGAGAACGGGGTTCTAGAGTTC CGCGGACTCTTCTTTCCTCCTTAGGACCCACTTTGCCCCGTCCCAGGGAGGAGAGCACCTATGTCTGCGC TGCGACCTCTCCTGCTTTTGCTGCTCCATCTGTGTCCGGGTCTAGGGCCGGGACATGGGAGTGAGGCAAA GGTCGTCCGGAGTTGTGCAGAGACTCGGCAGGTGCTGGGGGCCCGGGGATATAGCTTAAACCTAATCCCT CCCTCCCTCATCTCAGGTGAGCACCTTCAAGTCTGTCCTCAGGAGTACACCTGCTGTTCCAGTGAGACAG AGCAGAAGTTGATCAGGGATGCTGAAGTCACATTCCGTGGCCTGGTAGAGGACAGTGGCTCTTTCCTGAT TCACACACTGGCTGCCCGGCATAGAAAGTTTAATGAGTTTTTTCGGGAGATGCTGTCTATATCCCAGCAT TCTTTGGCTCAGCTCTTCTCGCATTCCTATGGTCGCCTGTATTCCCAGCACGCCGTCATCTTCAATAGCC TGTTTTCCGGCCTGCGGGACTACTATGAGAAGTCTGGCGAGGGGTTAGATGACACCTTGGCGGATTTCTG GGCACAGCTCCTGGAGAGAGCCTTCCCTTTGCTGCACCCACAATACAGTTTCCCTCCTGACTTCCTGCTC TGTCTTACTCGGCTTACTTCAACTGCTGATGGCTCTCTGCAGCCCTTCGGAGACTCACCCCGCCGCCTCC GCCTACAGATATCCAGGGCACTGGTGGCAGCCCGGGCCTTGGTCCAGGGTCTGGAGACTGGAAGAAATGT GGTCAGCGAAGCACTTAAGGTACCAGTGTTGGAAGGCTGCAGACAGGCCCTGATGCGTCTGATTGGCTGC CCTCTTTGTCGGGGAGTTCCTTCACTTATGCCCTGCCGGGGCTTCTGCCTCAATGTAGCCCATGGCTGCC TCAGCAGTAGGGGACTGGAGCCTGAATGGGGCGGATATCTGGATGGTCTCCTGCTGCTGGCTGAGAAACT CCAAGGACCCTTTTCCTTTGAACTGGCTGCTGAGTCCATTGGGGTGAAGATCTCAGAAGGTTTGATGCAC TTACAGGAAAACAGTGTAAAGGTGTCAGCGAAGGTGTTTCAGGAATGTGGGACCCCCCATCCCGTGCAGT CCCGCAGCCGGCGAGCACCAGCACCCCGAGAAGAGGCTAGCCGGTCGTGGAGGGCTTCAGCGGAGGAAGA ACGGCCCACAACAGCGGCAGGCACTAATCTGCACCGCTTGGTTTGGGAGCTCCGGGAGAGACTTAGCCGA GTACGGGGCTTCTGGGCTGGACTGCCTGTAACCGTATGTGGGGACTCCCGCATGGCGGCAGATCTCTCAC AGGAGACGGCACCCTGCTGGACTGGAGTTGGGAGAGGCCGGTATATGTCGCCTGTGGTCGTGGGCTCCTT GAATGAGCAGCTCCACAACCCGGAGTTGGATACTTCGAGTCCCGATGTCCCGACGCGGAGGCGGAGGCTA CATCTCCGAGCAGCTACTGCTCGAATGAAGGCGGCTGCGCTGGGTCAGGATCTTGACATGCATGATGCGG ATGAAGATGCCAGTGGCTCTGGAGGGGGACAGCAGTATGCAGACGACTGGAAAGCTGGGGCAGTGCCTGT GGTTCCCCCAGCCAGGCCTCCAAGGCCACCTCGTCCTCCTCGAAGGGACGGTCTTGGAGTGAGAGGAGGA AGTGGCAGTGCCAGATACAACCAGGGCCGAAGCAGGAATCTGGGGTCATCTGTTGGCCTCCATACCCCAC TTGTCCTCCTCCTCCTTCCCTCAGCCCTGACCCTGCTTGTACTTCGATAACGAGGGAGGGGCGCCCTAGC ATCAAAAGGCTTAGTGGCTCTTTTTCTTCCACCTCCCTCAGCCAGGCATGAGGAGGAGTGGAAGGGGGCT ACAGAGAGGGCAGAAAGGGACTTTGTGGGTGAATGGCTGGGGCCCCAAATCCAGGAGTTTCCAATTAATG GGGTGGGAGGTAGATGGGTTTCATAATATTTATTTTTTCATTTGGGAACTGGGGATATTTATTTAGGAAG GATGGTTCAGCTCTTTTAAATCTTTGGGCTCACTGATGGGGTGGGGGGTGGGACACGGGGTTGAAGGAAC TTGAAAGTGGGGAGGAATGGTACTATTGGCATGGGGGTACCTGGTATTGAAAATGGACACATAAACTAGC TGAGAGTGATGTCACTTGCCTGTAAACCCATTATTCTTTGGGATGCTGAGGCAGGAGGATTGAGAGTTAG GGACTAATAAAGCTAGGTGCTGACAGTAGAACAGGAAGGAGGGTAGAACCTGAGTTTTGTTGCCTCTTTT AAAATCATCTGCTTCTTAAGTATGGGGCGGGGGGAGGGACCCATCTAGCGACCTTTGCCATCCACATCCA TCACAAACTTCCACTTCATATTTCAGTGTTCATATCAGTGTCTATTGGTAAATAAAGAATTTATTGGAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5707617 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5707617 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19812 same embryo
	EMAGE:19814 same embryo
	EurExpress:euxassay_006296 same experiment
	MGI:4825155 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source